Evaluation of ergosterol content in the air of various environments

Gutarowska, Beata; Skóra, Justyna; Pielech‐Przybylska, Katarzyna

doi:10.1007/s10453-014-9344-4

Cited by 13 publications

(8 citation statements)

References 31 publications

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“…Low ergosterol levels (0.41–0.69 ng/m 3 ) in the air of studied rooms confirmed observations made by Gutarowska et al ( 2014 ) for rooms with low fungal contamination. An ergosterol concentration above 1 ng per m 3 can be considered as an indicator of excessive mould contamination of indoor air (10 3 cfu/m 3 ) (Gutarowska et al, 2014 ). Our study found a significant correlation between the number of fungi in the air of museums and archives and ergosterol concentration.…”

Section: Discussionsupporting

confidence: 91%

Assessment of microbiological contamination in the work environments of museums, archives and libraries

et al. 2015

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Museums, archives and libraries have large working environments. The goal of this study was to determine microbial contamination in these work places and estimate the influence of microclimatic parameters and total dust content on microbial contamination. In addition, research included evaluation of ergosterol concentration and fungal bioaerosol particle size distribution. Numbers of micro-organisms in the air and on the surfaces in museums were higher (2.1 × 102–7.0 × 103 cfu/m3 and 1.4 × 102–1.7 × 104 cfu/100 cm2, respectively) than in archives and libraries (3.2 × 102–7.2 × 102 cfu/m3 and 8.4 × 102–8.8 × 102 cfu/100 cm2, respectively). The numbers of micro-organisms detected in the tested museums, archives and libraries did not exceed occupational exposure limits proposed by Polish Committee for the Highest Permissible Concentrations and Intensities of Noxious Agents at the Workplace. The concentrations of respirable and suspended dust in museum storerooms were 2–4 times higher than the WHO-recommended limits. We found a correlation between microclimatic conditions and numbers of micro-organisms in the air in the tested working environments. In addition, a correlation was also found between ergosterol concentration and the number of fungi in the air. Fungi were the dominant micro-organisms in the working environments tested. Particles within the dominant fractions of culturable fungal aerosols sampled from museum storerooms had aerodynamic diameters between 1.1 and 2.1 µm.

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Section: Discussionsupporting

confidence: 91%

Assessment of microbiological contamination in the work environments of museums, archives and libraries

et al. 2015

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“…This method is also recommended for the monitoring of yeast and moulds in soil during composting or bioremediation [ 24 , 25 ]. Ergosterol can be detected using high performance liquid chromatography [ 26 ], gas chromatography with a flame ionization detector [ 27 ] or mass spectrometry [ 28 ], or by spectrophotometric UV [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Cationic and Neutral Gemini Surfactants on Conidia and Hyphal Forms of Aspergillus brasiliensis

Koziróg

Brycki

Pielech‐Przybylska

2018

IJMS

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This study investigates the biological activity of two cationic gemini surfactants, hexamethylene-1,6-bis-(N,N-dimethyl-N-dodecylammonium bromide) C6 and pentamethylene-1,5-bis-(N,N-dimethyl-N-dodecylammonium bromide) C5, and their two neutral analogs, hexamethylene-1,6-bis-(N-methyl-N-dodecylamine) (A6) and pentamethylene-1,5-bis-(N-methyl-N-dodecylamine) (A5). Experiments were performed with Aspergillus brasiliensis, which is used in the standard tests for biocides. The minimal inhibitory concentration (MIC) values for conidia and mycelium were determined using the dilution method. The viability of the conidia was evaluated using the plate count method. The dry mass of the mycelium was determined using the thermogravimetric method. Ergosterol was extracted from the mycelium and evaluated by gas chromatography. The effect of gemini surfactants on fungal morphology was observed using scanning electron microscopy. Cationic gemini surfactants were found to be active at lower concentrations compared to their non-ionic analogues, rapidly reducing the total number of conidia that were able to grow. They also decreased both the ergosterol content in the mycelium and its dry weight. These results suggest that cationic gemini surfactants C6 and C5 could have a wide range of practical applications as active compounds. However, it should be remembered that usage at too low concentrations, below the MIC, will only lead to short-term disturbances in the development of conidia and mycelium.

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“…ERG is the primary sterol present in the cell wall (membrane) of lamentous fungi [41,42] used to determine the quantity of moulds [43]. As it is can be seen in Table 2, the augmentative tendency of ERG content found in the fresh forage samples of orchardgrass from 2012 to 2013 was related at the higher rainy conditions in the second year, contributing to the occurrence and development of fungi.…”

Section: Discussionmentioning

confidence: 98%

Ergosterol and polyphenols: Potential biochemical indicators of silage safety

Alba-Mejía

Dohnal

Klíma

et al. 2020

Preprint

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Background: Silage, one of the most important feed sources for cattle, is vulnerable to contamination by spoilage moulds and mycotoxin production because ensilage forage crops are excellent substrates for fungal growth. Whereas there are many factors involved in mycotoxins contamination such as biological factors, harvesting, storage, and processing conditions, the climate is the most important factor. Silage additives can improve the safety of silage and inhibit moulds and other detrimental silage microorganisms; however, their efficacy varies with the type and level of toxigenic fungi contamination. Several studies provided evidence showing that the presence of various mycotoxins was correlated with the presence of ergosterol in cereals and feedstuffs. Therefore, ergosterol ( ERG ) has been suggested as a potential indicator of fungal contamination with polyphenols concentration analysis allowing accurate prediction on silage safety. The main goal of this study was to use the ERG and polyphenols content as potential biochemical indicators to predict the silage safety in ensiled grasses. The study was carried out using ten orchardgrass varieties (untreated and treated with biological and chemical additives). The determination of the samples was performed on high-performance liquid chromatography using UV detection (ERG) and spectrophotometer UV/VIS (polyphenols). Results: Interestingly, in the silage ‘Bepro’ was the unique variety that no presented content of ERG (0.0 mg kg -1 dry matter) in comparison with other varieties in the first cut in 2012. The biological additives reduced the content of ERG in both cuts in 2012 compared with untreated silage. In contrast, the chemical additives in silage grass had a negative effect ( P < 0.05) by increasing the ERG and polyphenols content in comparison with untreated silage. Conclusions: These results of this study provide indisputable evidence that silage contaminated with fungi increase the concentration of polyphenols, which prove a progressive deterioration of silage quality. In consequence, ERG and polyphenols seem to be relevant as a rapid method for screening silage safety.

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Evaluation of ergosterol content in the air of various environments

Cited by 13 publications

References 31 publications

Assessment of microbiological contamination in the work environments of museums, archives and libraries

Assessment of microbiological contamination in the work environments of museums, archives and libraries

Impact of Cationic and Neutral Gemini Surfactants on Conidia and Hyphal Forms of Aspergillus brasiliensis

Ergosterol and polyphenols: Potential biochemical indicators of silage safety

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