Evaluation of Erythrocyte Disorders With Mean Corpuscular Volume (MCV) and Red Cell Distribution Width (RDW)

Monzon, Carlos M.; Beaver, B D; Dillon, T D

doi:10.1177/000992288702601203

Cited by 32 publications

(24 citation statements)

References 12 publications

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“…Abnormalities in RDW (measure of erythrocyte size variation) occur during iron-deficient erythropoiesis, especially when hemoglobin content (MCHC) falls. 29,30 Cord zinc protoporphyrin/heme ratios in diabetes KB Lesser et al ZnPP/H ratios were similar in those with excellent and poor control. Because of limited number of women with excellent control, the study may have had insufficient power to show lower ZnPP/H ratios with better glycemic control from the glucose logs.…”

Section: Discussionmentioning

confidence: 95%

Elevated zinc protoporphyrin/heme ratios in umbilical cord blood after diabetic pregnancy

2006

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Objective: Offspring of diabetes patients may suffer from tissue iron deficiency. Erythrocyte zinc protoporphyrin/heme (ZnPP/H) ratios measure impaired iron status. The aim of the study was to examine whether cord ZnPP/H ratios were associated with pregnancy glycemic control.Methods: ZnPP/H was measured in cord blood from 31 pregnancies with insulin-treated diabetes (diabetes group) and compared to population normal values. Maternal glycemic control was assessed by daily glucose log, glycosylated hemoglobin and birth weight.Results: Median cord ZnPP/H was higher in the diabetes group than the population normal values (106 (65.2 to 146.8) mM/M vs 68.2 (37.6 to 98.8) mM/M, P<0.0001). Ratios were directly correlated to surrogates of control (glycosylated hemoglobin, P ¼ 0.05, and birth weight, P<0.04). Cord ZnPP/H ratios from pregnancies with pre-existing and gestational diabetes were similar.Conclusion: Because cord ZnPP/H was higher in large offspring of diabetic pregnancy, it might identify greater iron utilization for fetal erythropoiesis.

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Section: Discussionmentioning

confidence: 95%

Elevated zinc protoporphyrin/heme ratios in umbilical cord blood after diabetic pregnancy

2006

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“…At approximately PND 4, both RDW and ZnPP/H levels peaked at relatively high values in our rat population. In humans, the RDW indicates variations in red cell size and increases when iron-deficient, microcytic cells join circulating normocytic cells [19]. In humans, the RDW increases as the first clinical sign of functional iron deficiency, followed by changes in ZnPP/H which indicate incomplete incorporation of iron into heme [3,19].…”

Section: Discussionmentioning

confidence: 99%

Iron-Deficient Erythropoiesis in Neonatal Rats

Dubuque

Dvořák²,

Woodward³

et al. 2002

Neonatology

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Anemia in premature infants is extremely common. Precise quantitation of iron status and determination of iron incorporation into erythrocytes are important in monitoring therapy for anemia in premature infants, especially when treating with recombinant human erythropoietin (rhEPO). However, the traditional indices of the iron status have limited usefulness in this population. The goal of the current work is to develop an experimental animal model system that addresses the clinical issue relating to quantitation of iron delivery to erythrocytes. We first determined normal hematological values for nontreated, dam-suckled Sprague-Dawley rats by measuring markers of erythropoiesis and iron status during the first 12 postnatal days (PND). The experimental group of rats were administered parenteral rhEpo (430 IU·kg^–1 · day^–1) for 8 days (from PND 4 until PND 12) in the absence (rhEpo^–Fe) or presence (rhEpo^+Fe) of oral iron supplementation (6 mg·kg^–1·day^–1). Rat pups receiving oral iron only (control^+Fe) and pups that were sham fed with the orogastric tube (control^–Fe) were included as controls. Hematological parameters were measured in blood and bone marrow. In a pattern similar to that seen in premature infants during the first 2 months of life, the levels of these hematopoietic markers were dynamic and changed during the first 12 PND. After challenging experimental animals with subcutaneous rhEpo, evidence of iron-deficient erythropoiesis was seen in the rhEpo^–Fe group. Red blood cell levels and absolute reticulocyte counts were higher in both groups receiving rhEpo as compared with the controls. However, the rhEpo^–Fe group experienced a lower hemoglobin level, a lower mean red cell volume, a greater red cell distribution width, and a higher zinc protoporphyrin/heme (ratio than the rhEpo^+Fe group. The neonatal rat is an excellent model of iron-deficient erythropoiesis and will be useful in designing future mechanistic studies examining the interplay between iron and erythropoiesis in the anemic, iron-challenged premature neonate.

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“…This finding may indicate microcytic erythrocytes before the decrease of MCV. Although it has not been found yet in studies supporting this correlation in dogs, it is known that human patients with low MCV but high RDW often have iron deficiency or red blood cell fragmentation (Monzon et al, 2006).…”

Section: Resultsmentioning

confidence: 90%

“…Automated cell analyzers show the distribution of erythrocyte volume in 95% of the blood sample and determine its coefficient of variation (RDW-CV), which is calculated from an equation. This index is related to the MCV and it is expressed in percentage (Balarin et al, 2001;Monzon et al, 2006). The RDW-SD is the standard deviation of the erythrocyte distribution curve, expressed in fentoliter (fL), being a direct measure, which is independent of MCV and so accurately demonstrates the variation in size of an erythrocyte population (Walters, 2002).…”

Section: Introductionmentioning

confidence: 99%

Behavior of red cell distribution width (RDW) in a population of anemic and non-anemic dogs from an ambulatory care unit.

Souza¹,

Pereira²,

Campos³

et al. 2017

Revista Brasileira De Ciência Veterinária

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ResumoO objetivo deste estudo foi avaliar o comportamento do índice de anisocitose na interpretação do hemograma de cães anêmicos e não anêmicos. Atualmente, contadores hematológicos automatizados veterinários fornecem alguns índices que não são calculados quando o hemograma é confeccionado manualmente. A saber: RDW-CV (Amplitude de distribuição de hemácias como coeficiente de variação) e RDW-SD (Amplitude de distribuição de hemácias como desvio-padrão). Tais índices levaram a uma nova abordagem do paciente com anemia permitindo que a anisocitose fosse mensurada de uma forma quantitativa. Foram avaliados 454 hemogramas processados no período de setembro de 2009 a março de 2011. Foram calculados média e desvio padrão e uma análise de variância foi realizada. Os cães anêmicos (125/454) apresentaram altos valores de RDW enquanto que, em animais não anêmicos, esse índice permaneceu dentro dos valores de normalidade previamente estabelecidos. O índice também estava alto em animais com anisocitose observada à microscopia. Houve uma diferença significante entre os índices dos animais que apresentaram Volume Globular Médio (VGM) elevado e normal. Concluiu-se que os índices RDW-CV e RDW-SD foram sensíveis a pequenas variações no tamanho das hemácias e na heterogeneidade, sendo mais precisos que a observação microscópica para se detectar anisocitose e mais sensível que VGM para variação eritrocitária.Palavras-chave: anemia, anisocitose, contador hematológico automatizado, hematologia. AbstractThe aim of this study was to evaluate the behavior of the red cell distribution width (RDW) in the interpretation of the Complete Blood Count (CBC) from anemic and non-anemic dogs. Currently, automated veterinary blood cell analyzers provide hematologic indices not calculated in manually performed CBC, such as Red Blood Cell Distribution Width measured by Variation Coefficients (RDW-CV) and Red Blood Cell Distribution Width measured by Standard Deviation (RDW-SD), which has led to new of September 2009 to March 2011. Mean and standard deviation were calculated and variance analysis was performed. Anemic dogs (125/454) presented higher values for RDW indices. In non-anemic animals, these indices were in accordance with the normal values previously established. Both indices were higher in dogs with microscopic observation of anisocytosis. There was a significant difference among índices of the animals which showed normal and increased Mean Corpuscular Volume (MCV). We concluded that RDW-CV and RDW-SD indices were sensitive to small variations on erythrocyte size and heterogeneity, being more accurate than microscopic observations to detect anisocytosis and more sensitive than MCV to erythrocyte variation.

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Evaluation of Erythrocyte Disorders With Mean Corpuscular Volume (MCV) and Red Cell Distribution Width (RDW)

Cited by 32 publications

References 12 publications

Elevated zinc protoporphyrin/heme ratios in umbilical cord blood after diabetic pregnancy

Elevated zinc protoporphyrin/heme ratios in umbilical cord blood after diabetic pregnancy

Iron-Deficient Erythropoiesis in Neonatal Rats

Behavior of red cell distribution width (RDW) in a population of anemic and non-anemic dogs from an ambulatory care unit.

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