Evaluation of Estrogen Receptor α and ß Genes Expression in Normal and Neoplastic Mammary Gland in Dogs by Real-time PCR

Abstract

Background: Estrogen and progesterone play a key role in mammary tumorigenesis of female dogs. Malignant tumors are considered hormone-dependent neoplasms, which may reappear after surgical removal or might spread to other organs such as liver, lymph nodes, and lungs. Objective: The purpose of this study was to evaluate the expression of estrogen receptor α and ß genes in normal and neoplastic mammary glands of female dogs. Method: Ten neoplastic tumors (benign and malignant) and 10 normal samples were selected. After RNA extraction and cDNA synthesis, ERα and ERβ genes were amplified by Real-time PCR based on SYBR green related to β-actin reference gene. Result: The obtained C t and ∆C t values were analyzed by GLM procedure of SAS software v9.2. The results showed that the expression of ERα and ERβ genes in neoplastic cases was over-and down-expressed respectively as compared with normal samples (p < 0.01). Conclusion: The findings indicated that these genes could be used as reference genes for screening and also for early diagnosis of patients, and this method could be helpful for patient selection and monitoring of the anti-estrogen therapy.

Expression and prognostic value of TRPM7 in canine mammary tumours

“…Canine mammary gland tumour (CMTs) account for 70% of all tumours in intact female dogs 1,2 and can be benign or malignant in nature. CMTs are hormone‐dependent and might recur after surgical removal or metastasize to other organs, in particular, the lymph node and lungs 3 . The prognostic factors of cancer include histological type, tumour grade, mode of tumour growth, lymph node status and tumour size 4 .…”

“…CMTs are hormone‐dependent and might recur after surgical removal or metastasize to other organs, in particular, the lymph node and lungs. 3 The prognostic factors of cancer include histological type, tumour grade, mode of tumour growth, lymph node status and tumour size. 4 These clinicopathological factors are important for evaluating and determining a prognosis as well as predicting the molecular cancer behaviour.…”