Evaluation of Eugenia uniflora L. leaf extracts obtained by pressurized liquid extraction: Identification of chemical composition, antioxidant, antibacterial, and allelopathic activity

Bagatini, Lucíola; Zandoná, Giovana Paula; Hoffmann, Jéssica Fernanda; Cardoso, Juliane de Souza; Teixeira, Fernanda Cardoso; Moroni, Liziane Schittler; Junges, Alexander; Kempka, Aniela Pinto; Stefanello, Francieli Moro; Rombaldi, César Valmor

doi:10.1016/j.scp.2023.101214

Cited by 8 publications

(7 citation statements)

References 65 publications

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“…Moreover, the ion fragments produced with m / z 179 and 151 were compared with literature data and attributed to the confirmation of the flavonol core (kaempferol, quercetin, or myricetin) ( Table 3 ). All these compounds were identified previously in E. uniflora [ 15 , 44 , 45 , 46 , 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…Gallotannins were detected and were distinguished by their characteristic fragment ion spectra, yielding sequential losses of galloyl (m/z 152) and gallate (m/z 170) residues. The digalloyl-hexoside derivatives (4,7,(9)(10)(11) were assinalated based on the [M−H] − ion at m/z 483 or your derivatives and MS/MS produced as typical product ions at m/z 313, 271, 211, 193, 169, and 125 [15,[44][45][46]. Additionally, the compounds citric acid (2), gallic acid (3), 5-O-Caffeoylquinic acid (5), 5-O-Coumaroylquinic acid (6) were identified based on [M−H] − ions at m/z 191, 169, 353, 337, respectively.…”

Section: Identification Of Other Phenolic Compoundsmentioning

confidence: 99%

“…Additionally, the compounds citric acid (2), gallic acid (3), 5-O-Caffeoylquinic acid (5), 5-O-Coumaroylquinic acid (6) were identified based on [M−H] − ions at m/z 191, 169, 353, 337, respectively. MS/MS data of these ions were compared with the literature data [15,[44][45][46][47].…”

Section: Identification Of Other Phenolic Compoundsmentioning

confidence: 99%

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Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation

Tenório,

Dantas,

Abreu

et al. 2024

Molecules

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The content of chemical constituents in Eugenia uniflora leaf extracts correlates positively with biological activities. The experimental objective was to carry out the phytochemical screening and purification of the major polyphenols from the leaves of E. uniflora. In addition, the anti-Candida activity of the hydroalcoholic extract, fraction, subfractions and polyphenols purified were evaluated. After partitioning of the extract with ethyl acetate, the fractions were chromatographed on Sephadex® LH-20 gel followed by RP-flash chromatography and monitored by TLC and RP-HPLC. The samples were characterized by mass spectrometry (LC-ESI-QTOF-MS2) and subjected to the microdilution method in 96-well plates against strains of C. albicans, C. auris, and C. glabrata. Myricitrin (93.89%; w/w; m/z 463.0876), gallic acid (99.9%; w/w; m/z 169.0142), and ellagic acid (94.2%; w/w; m/z 300.9988) were recovered. The polyphenolic fraction (62.67% (w/w) myricitrin) and the ellagic fraction (67.86% (w/w) ellagic acid) showed the best antifungal performance (MIC between 62.50 and 500 μg/mL), suggesting an association between the majority constituents and the antifungal response of E. uniflora derivatives. However, there is a clear dependence on the presence of the complex chemical mixture. In conclusion, chromatographic strategies were effectively employed to recover the major polyphenols from the leaves of the species.

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Section: Resultsmentioning

confidence: 99%

Section: Identification Of Other Phenolic Compoundsmentioning

confidence: 99%

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Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation

Tenório,

Dantas,

Abreu

et al. 2024

Molecules

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“…At the same time, bacteriocin enters bacterial cells or passes through biofilms using short-term changes in membrane permeability, then accumulates in the bacterial plasma membrane using its hydrophobicity. This causes various toxic effects, hindering bacterial metabolism and interfering with various metabolic pathways [ 135 ]. Therefore, use of bacteriocins in combination with plant extracts may be a promising method of maintaining the quality of food products and prolonging their shelf life.…”

Section: Combinations Of Bacteriocins and Chemical Substancesmentioning

confidence: 99%

Potential Impact of Combined Inhibition by Bacteriocins and Chemical Substances of Foodborne Pathogenic and Spoilage Bacteria: A Review

Yu,

Guo,

Liu

et al. 2023

Foods

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In recent years, food safety caused by foodborne pathogens and spoilage bacteria has become a major public health problem worldwide. Bacteriocins are a kind of antibacterial peptide synthesized by microbial ribosomes, and are widely used as food preservatives. However, when used individually bacteriocins may have limitations such as high cost of isolation and purification, narrow inhibitory spectrum, easy degradation by enzymes, and vulnerability to complex food environments. Numerous studies have demonstrated that co-treatment with bacteriocins and a variety of chemical substances can have synergistic antibacterial effects on spoilage microorganisms and foodborne pathogens, effectively prolonging the shelf life of food and ensuring food safety. Therefore, this paper systematically summarizes the synergistic bacteriostatic strategies of bacteriocins in combination with chemical substances such as essential oils, plant extracts, and organic acids. The impacts of bacteriocins when used individually and in combination with other chemical substances on different food substrates are clarified, and bacteriocin–chemical substance compositions that enhance antibacterial effectiveness and reduce the potential negative effects of chemical preservatives are highlighted and discussed. Combined treatments involving bacteriocins and different kinds of chemical substances are expected to be a promising new antibacterial method and to become widely used in both the food industry and biological medicine.

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“…Natural phytochemical compounds are important alternatives for the development of the pharmaceutical industry through phytomedicine [18]. Polyphenols are compounds with unique chemical and biological properties, notably as bioactive antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Extraction of Bioactive Compounds from Prestonia mollis Leaves and Their Impregnation into Polylactic Acid Using High-Pressure Technologies: Potential for Biomedical Application

Burgos-Briones,

Verano-Naranjo,

Cejudo-Bastante

et al. 2023

Antioxidants

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Enhanced solvent extraction (ESE) and pressurized liquid extraction (PLE) have been used for the first time to obtain antioxidant compounds from Prestonia mollis leaves. The effects of pressure (100–250 bar), temperature (55–75 °C) and the composition of the extraction solvent (ethanol, water and hydroalcoholic mixtures) were evaluated according to multilevel factorial designs. PLE provided the largest extraction yields compared to ESE, as well as a greater impact of the operating conditions studied. The highest total phenolic content was obtained when using a hydroalcoholic mixture (CO2/ethanol/water 50/25/25) through ESE at 100 bar and 75 °C. The antioxidant capacity of this extract is related to higher concentration levels of the identified flavonoids: Quercetin 3-O-xylosyl-rutinoside, Kaempferol 3-(2G-apiosylrobinobioside) and Kaempferol 4′-glucoside 7-rhamnoside. This extract was tested for the supercritical impregnation of polylactic acid (PLA), which is a polymer widely used in the biomedical industry. The influence of pressure (100–400 bar), temperature (35–55 °C), amount of extract (3–6 mL) and impregnation time (1–2 h) have been evaluated. The best results were obtained by impregnating 3 mL of extract at 100 bar and 55 °C for 2 h, achieving 10% inhibition with DPPH methods. The extract presented a potentially suitable impregnation of PLA for biomedical applications.

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Evaluation of Eugenia uniflora L. leaf extracts obtained by pressurized liquid extraction: Identification of chemical composition, antioxidant, antibacterial, and allelopathic activity

Cited by 8 publications

References 65 publications

Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation

Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation

Potential Impact of Combined Inhibition by Bacteriocins and Chemical Substances of Foodborne Pathogenic and Spoilage Bacteria: A Review

Extraction of Bioactive Compounds from Prestonia mollis Leaves and Their Impregnation into Polylactic Acid Using High-Pressure Technologies: Potential for Biomedical Application

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