Evaluation of ex vivo effectiveness of commercial desensitizing dentifrices

Mockdeci, Hanny Reis; Polonini, Hudson; Martins, Isadora Conde Ferreira; Granato, Ana-Paula; Raposo, Nádia Rezende Barbosa; Chaves, Maria das Graças Afonso Miranda

doi:10.4317/jced.53040

Cited by 16 publications

(30 citation statements)

References 22 publications

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“…In fact, as can be seen in the SEM photomicrographs, dentin tubules was partially occluded in both NaF-treated groups. This result is similar to in vitro and in vivo studies that evaluated the efficacy of fluoride products in the management of DH and found that these products decrease dentin permeability but produced only partial occlusion of dentin tubules (17,21).…”

Section: Discussionsupporting

confidence: 85%

Calcium Pre-Rinse: Effect on permeability of dentin tubules by fluoride rinse

Braga

Carvalho

et al. 2019

J Clin Exp Dent

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Background The aim of this study was to evaluate whether calcium (CaL) solution would enhance the capacity of sodium fluoride (NaF) solution in reducing the permeability of hypersensitive dentin. Material and Methods Thirty-two Wistar rats ingested for 45 days acidic isotonic drink (Gatorade, pH 2.7) ad libitum to induce dental erosion. Then, molar teeth received a cold stimulus to confirm the presence and score the intensity of dentin hypersensitivity based on body contraction and noise. Animals were allocated to four groups (n=8), according to the solution(s) applied in the oral cavity: NaF (12 mmol/L, 1 min); CaL (150 mmol/L, 1 min); CaL followed by NaF (CaF+NaF, 1 min each); distilled water (DW, 1 min, as negative control). The animals were euthanized and the mandibles dissected into hemimandibles, which were sealed with sticky wax, except for the occlusal surface of the molar teeth. The samples were immersed in 10% copper sulphate solution and in 1% dithioxamide alcoholic solution (25 min each). The samples were sectioned longitudinally and imaged under optical microscope. Then, dentin permeability was measured as the area of copper ion penetration, using ImageJ software. Photomicrographs were obtained by scanning electron microscopy. Results 68.7% of animals had body contraction associated or not with noise. One-way ANOVA followed by Tukey´s test indicated that groups treated with NaF solution, whether or not preceded by CaL solution, presented lower permeability than the remaining groups [CaL+NaF: 3405.7 μm2 (±1796.4); NaF: 4111.7 μm2 (±2450.6); CaL: 42254.6 μm2 (±30399.2); DW: 37064.6 μm2 (±21994.4)]. Photomicrographs showed that CaL+NaF group presented an increased proportion of occluded dentin tubules in comparison to the NaF-only group. Conclusions Although qualitatively there seems to be a benefit in using CaL pre-rinse, this solution did not quantitatively enhance the capacity of NaF in reducing permeability of hypersensitive dentin. Key words: Dentin hypersensitivity, Fluoride, Calcium lactate, Animal model.

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Section: Discussionsupporting

confidence: 85%

Calcium Pre-Rinse: Effect on permeability of dentin tubules by fluoride rinse

Braga

Carvalho

et al. 2019

J Clin Exp Dent

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“…5 The particle size distribution, effective diameter, and polydispersity index were also measured at 22°C (Phase Analysis Light Scattering-PALS, 90 Plus, Brookhaven Instruments Corporation, Holtsville, NY, United States) after dilution (250 mg/L) of slurries in distilled water. 19 Ten replications for the particle analyses were evaluated (n = 10).…”

Section: Chemical and Physical Analyses Of The Toothpaste Slurriesmentioning

confidence: 99%

Effects of 1450-ppm Fluoride-containing Toothpastes Associated with Boosters on the Enamel Remineralization and Surface Roughness after Cariogenic Challenge

et al. 2020

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Objectives This in vitro study investigated the remineralization potential of 1450 ppm, fluoride-containing toothpastes containing different active remineralization agents after cariogenic challenge with pH cycling. The enamel surface roughness after brushing and the chemical and physical characteristics of the toothpastes tested were also analyzed. Materials and Methods Fifty-six bovine enamel blocks were obtained (4 × 4 × 6 mm) and divided into three thirds: intact (untreated), demineralized (artificial caries lesion), and treated (caries lesion, pH cycling, and brushing with dentifrices). Seven commercially available fluoride toothpastes (1450 ppm F): three with anti-erosion claims ( Candida Professional [CPP], Colgate Total 12 Daily Repair [CDR], Regenerate Enamel Science [RES]); three with desensitizing claims (Bianco Pro Clinical [BPP], Elmex Sensitive [ESS], and Regenerador Diário DentalClean [RDC]); and one standard regular-fluoride toothpaste Colgate Total 12 (CTT) were selected. During pH cycling (demineralization 6 h/remineralization 18 h) for 7 days, the treated third was brushed with the different dentifrices for 10 minutes in a brushing machine before immersion in a remineralizing solution. The Knoop hardness (25 g, 10 second of the surface, and longitudinal section were then evaluated at eight depths (10 to 330 μm). Mean and percentage of surface hardness recovery (% SHR) were calculated. Surface enamel roughness (Ra) was also evaluated. The pH, %weight of particles, zeta potential, and polydispersity index of toothpaste slurries were also evaluated. Statistical Analysis Data were statistically analyzed (ANOVA/Tukey, 5%). Results The %SHR of CPP was significantly lower than the others (p < 0.05). The enamel subsurface was more effectively remineralized when treated with BPP, ESS, and RDC. The surface roughness was higher when the demineralized third was treated with CTT, RDC, and RES and after the cariogenic challenge (p < 0.05). For some of the products tested, there was no relationship between surface remineralization and subsurface remineralization. Although toothpastes CPP and RDC present the lowest %SHR Abstract Keywords ► enamel ► hardness ► roughness ► toothpaste ► tooth remineralization

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“…O TP mostra o tamanho das partículas presentes em suspensão (MOCKDECI et al, 2017). Diversos fatores podem influenciar esse parâmetro, como: a concentração do surfactante; tipo de lipídio; propriedades do ativo a ser encapsulado e características de produção (tempo, temperatura e pressão) (MARCATO et al, 2009).…”

Section: Características Do Ensaiounclassified

“…Diversos fatores podem influenciar esse parâmetro, como: a concentração do surfactante; tipo de lipídio; propriedades do ativo a ser encapsulado e características de produção (tempo, temperatura e pressão) (MARCATO et al, 2009). De acordo com Toledo (2015), a técnica classicamente utilizada para fazer essa medida é a de No que se refere ao PZ, ele reflete a carga superficial das partículas e pode ser influenciado pela composição das mesmas, pelo meio de dispersão, pelo pH e pela força iônica presentes na solução (ANGELIERI, 2013;MOCKDECI et al, 2017). Geralmente, partículas com valores de PZ de pelo menos ± 25 mV apresentam boa estabilidade coloidal quando em suspensão (MOCKDECI et al, 2017).…”

Section: Características Do Ensaiounclassified

Nanopartículas lipídicas sólidas contendo óleo essencial de Melaleuca alternifolia Cheel como plataforma inovadora no tratamento da candidíase orofaríngea

Gonçalves¹

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Espécies do gênero Candida spp. são frequentemente encontradas na microbiota bucal vivendo de forma comensal; mas podem mudar para o estado patogênico e gerar candidíase orofaríngea, que pode evoluir para candidemia. Apesar da importância clínica e epidemiológica dessas infecções, as opções terapêuticas são limitadas, o que tem estimulado a busca por novos medicamentos, dentre eles os de origem vegetal e o emprego da nanotecnologia como estratégia tecnológica. Nesse contexto, o presente estudo teve como objetivo desenvolver uma plataforma inovadora para o tratamento da candidíase orofaríngea, constituída por um hidrogel contendo o óleo de Melaleuca alternifolia Cheel (OM) encapsulado em nanopartículas lipídicas sólidas (NLS). A composição química do óleo foi avaliada por cromatografia gasosa acoplada à espectrometria de massas. As NLS foram caracterizadas em relação ao tamanho hidrodinâmico médio das partículas (TP), índice de polidispersividade (PdI), potencial Zeta (PZ), eficiência de encapsulação (EE), morfologia, estabilidade, potencial antioxidante e citotoxicidade in vitro em linhagem celular de fibroblastos murinos (L929). As NLS contendo OM (OM-NLS) foram incorporadas em hidrogel e, em seguida, verificou-se a atividade antifúngica frente a leveduras padrão de C. albicans, C. glabrata, C. krusei e C. guilliermondii. Por fim, a permeação ex vivo do OM a partir do hidrogel, utilizando mucosa jugal suína foi analisada. Foram identificados treze constituintes no OM, cuja composição percentual atendeu aos padrões descritos pela ISO 4730: 2017. Quanto à caracterização das OM-NLS, os valores para TP, PdI e PZ foram 135,9 ± 6,65; 0,19 ± 0,04 e -31,07 ± 1,05, respectivamente. As OM-NLS mostraram uma EE de 92,74% ± 0,012% e revelaram uma forma esférica. Além disso, a atividade antioxidante variou de 14,1% a 17,2% e foi encontrado efeito citotóxico contra a linhagem celular L929 (IC50 = 77,4 µg/mL). Por pelo menos 120 dias, não foram observadas mudanças significativas nos valores da média de TP e PdI para OM-NLS, e o hidrogel contendo OM-NLS permaneceu estável por minimamente 30 dias, ambos sob refrigeração. OM-NLS e hidrogel contendo OM-NLS exibiram efeito fungicida (concentração fungicida mínima variou entre 5,60 e 11,20 mg/mL; e 5,53 e 11,06 mg/mL, respectivamente); e permeação transmucosa (70,52% ± 3,85%). Concluiu-se que o hidrogel contendo OM-NLS apresentou efeito fungicida superando o OM, estabilidade durante o período de avaliação e foi permeável pela mucosa jugal suína, podendo ser, portanto um sistema de entrega promissor na candidíase orofaríngea.

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Evaluation of ex vivo effectiveness of commercial desensitizing dentifrices

Cited by 16 publications

References 22 publications

Calcium Pre-Rinse: Effect on permeability of dentin tubules by fluoride rinse

Calcium Pre-Rinse: Effect on permeability of dentin tubules by fluoride rinse

Effects of 1450-ppm Fluoride-containing Toothpastes Associated with Boosters on the Enamel Remineralization and Surface Roughness after Cariogenic Challenge

Nanopartículas lipídicas sólidas contendo óleo essencial de Melaleuca alternifolia Cheel como plataforma inovadora no tratamento da candidíase orofaríngea

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