According to the seismic anisotropy theory and the test observation, this paper puts forward the conception of the ground micro tremor anisotropy, and researches the method of the micro tremor test analysis about the anisotropy of the ground dynamic characteristics. Given the anisotropic characteristics of the ground motion, it analyzes the influence of the ground motion anisotropy on the seismic dynamic response of the structure, and the existent problems about the selection and adjustment of the existing seismic time history in the structure analysis process. Finally, this paper puts forward the new selection and adjustment solution of ground motion input based on the ground micro tremor test to determine the ground dynamic characteristics and the ground anisotropy.