Evaluation of extracellular polymeric substances matrix volume, surface roughness and bacterial adhesion property of oral biofilm

Li, Heng; Liu, He; Zhang, Lei; Hieawy, Ahmed; Shen, Ya

doi:10.1016/j.jds.2022.12.022

Cited by 31 publications

(27 citation statements)

References 28 publications

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“…Organized biofilms harbor the microorganisms responsible for oral diseases such as caries. S. mutans , for instance, predominantly reside in biofilms established on tooth surfaces, commonly known as dental plaque, biofilm, or cariogenic biofilms 18,19 . These bacteria are adept at thriving in acidic surroundings and envelop themselves in a matrix rich in EPS 4 .…”

Section: Discussionmentioning

confidence: 99%

“…Mature oral biofilms demonstrate significant challenges in dentistry, as they can resist antimicrobial compounds. 18,19 As known, bacteria in a thick and multilayered mature biofilm can withstand high antibiotic concentrations. 2 Therefore, biofilm eradication may be substantially more difficult.…”

Section: Discussionmentioning

confidence: 99%

“…S. mutans, for instance, predominantly reside in biofilms established on tooth surfaces, commonly known as dental plaque, biofilm, or cariogenic biofilms. 18,19 These bacteria are adept at thriving in acidic surroundings and envelop themselves in a matrix rich in EPS. 4 In this context, S. mutans produces an abundant EPS matrix that restricts the spread of antimicrobial agents into the biofilms, 3,21,22 complicating the antibacterial action of several available therapeutic agents, including aPDT.…”

Section: Discussionmentioning

confidence: 99%

“…Implementing nanotechnology in preventive dentistry provides an excellent opportunity to improve the efficiency and potency of many ongoing strategies 18,19 . Several investigations have proposed multiple photosensitizer nanoplatforms to enhance the effectiveness of aPDT 17 .…”

Section: Introductionmentioning

confidence: 99%

“…Implementing nanotechnology in preventive dentistry provides an excellent opportunity to improve the efficiency and potency of many ongoing strategies. 18,19 Several investigations have proposed multiple photosensitizer nanoplatforms to enhance the effectiveness of aPDT. 17 In our previous study, a photosensitizer nanoplatform combining TBO and magnetic nanoparticles (MagTBO) carried into a microemulsion was developed and optimized.…”

mentioning

confidence: 99%

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Magnetic‐based photosensitizer to improve the efficiency of antimicrobial photodynamic therapy against mature dental caries‐related biofilms

Balhaddad,

Mokeem,

Alsahafi

et al. 2023

MedComm – Biomaterials and Applications

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Antimicrobial photodynamic therapy (aPDT) shows promise in eliminating oral pathogens without inducing microbial resistance. Yet, it faces limited biofilm penetration by the photosensitizer, which hinders its efficacy against mature, thick biofilms. This study assesses the effectiveness of the MagTBO (magnetic nanoparticles and toluidine‐blue ortho) nanoplatform against mature Streptococcus mutans biofilms associated with dental caries. The study employs constant‐depth film fermenter (CDFF) models, known as “artificial mouth,” to replicate caries processes and allow biofilm growth over teeth. This method enables the evaluation of biofilm‐induced mineral loss under cariogenic challenge over 5 and 10 days. The study shows that MagTBO improves aPDT's effectiveness against highly mature and complex biofilms over 5 and 10 days. However, the biofilm reduction unaffected the mineral content in the underlying dentin. This presents a promising approach for clinical aPDT protocols. However, it is crucial to acknowledge that these findings are based on in vitro studies and may necessitate further clinical confirmation. In summary, our data indicate that magnetic‐based photosensitizers enhance the modulation of pathogenic oral biofilms by aPDT, offering potential advancements in clinical aPDT protocols.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Magnetic‐based photosensitizer to improve the efficiency of antimicrobial photodynamic therapy against mature dental caries‐related biofilms

Balhaddad,

Mokeem,

Alsahafi

et al. 2023

MedComm – Biomaterials and Applications

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A Systematic Study of Staphylococcus aureus Biofilm Formation on Thiol‐Ene Polymers: Toward the Development of Microfluidic Bacterial Biofilm Models

Amorim,

Rosqvist,

Cruz

et al. 2025

Adv Materials Inter

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Global antimicrobial resistance poses a major threat to human health and is largely driven by bacterial biofilms, which demonstrate significantly greater antibiotic resistance than planktonic bacteria. While most biofilm research targets the development of antibiofilm surfaces, materials that intentionally promote biofilm formation are crucial for creating screening tools to discover new antibiofilm agents. The transition from static to flow‐through assay systems is also necessary to increase the methodological readiness of antibiofilm research. This study evaluates the feasibility of an emerging polymer platform, off‐stoichiometry thiol‐ene (OSTE), in supporting Staphylococcus aureus biofilms. OSTE polymers provide versatile options for rapid prototyping of microfluidic devices, with unique opportunities for on‐chip oxygen management. Here, the impacts of OSTE's key materials properties on S. aureus adhesion, biofilm viability, biomass, and metabolic activity are systematically examined in comparison to polystyrene, the current standard in microwell plate‐based biofilm assays. Additionally, the composition of the extracellular polymer substances matrix and antimicrobial susceptibility are investigated to determine the most suitable OSTE composition for microfluidic S. aureus biofilm cultures. The results confirm compatibility with S. aureus biofilms, supported by atomic force microscopy analysis of biofilm morphologies under static and microfluidic conditions.

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The Study of Fouling Behavior for the Model Binary/Ternary Mixture Solution

Liu,

Wang,

Wang

2024

Environmental Science and Engineering

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Evaluation of extracellular polymeric substances matrix volume, surface roughness and bacterial adhesion property of oral biofilm

Cited by 31 publications

References 28 publications

Magnetic‐based photosensitizer to improve the efficiency of antimicrobial photodynamic therapy against mature dental caries‐related biofilms

Magnetic‐based photosensitizer to improve the efficiency of antimicrobial photodynamic therapy against mature dental caries‐related biofilms

A Systematic Study of Staphylococcus aureus Biofilm Formation on Thiol‐Ene Polymers: Toward the Development of Microfluidic Bacterial Biofilm Models

The Study of Fouling Behavior for the Model Binary/Ternary Mixture Solution

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