Evaluation of Extracellular Subviral Particles of Dengue Virus Type 2 and Japanese Encephalitis Virus Produced by<i>Spodoptera frugiperda</i>Cells for Use as Vaccine and Diagnostic Antigens

Kuwahara, Masaki; Konishi, Eiji

doi:10.1128/cvi.00087-10

Cited by 36 publications

(26 citation statements)

References 52 publications

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“…In this study, we compared the antibody responses induced by infectious DENV virions, recombinant DENV subviral particles (prME), and soluble E dimers (E85). Coexpression of prM and E of dengue virus and other flaviviruses using heterologous expression systems results in the assembly of recombinant subviral particles (74)(75)(76)(77)(78)(79). These subviral particles are smaller than dengue virus virions, have an icosahedral symmetry of T ϭ 1 instead of T ϭ 3, and may display E proteins with different organizations.…”

Section: Discussionmentioning

confidence: 99%

An Alphavirus Vector-Based Tetravalent Dengue Vaccine Induces a Rapid and Protective Immune Response in Macaques That Differs Qualitatively from Immunity Induced by Live Virus Infection

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Sariol

Mattocks

et al. 2013

J Virol

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Despite many years of research, a dengue vaccine is not available, and the more advanced live attenuated vaccine candidate in clinical trials requires multiple immunizations with long interdose periods and provides low protective efficacy. Here, we report important contributions to the development of a second-generation dengue vaccine. First, we demonstrate that a nonpropagating vaccine vector based on Venezuelan equine encephalitis virus replicon particles (VRP) expressing two configurations of dengue virus E antigen (subviral particles [prME] and soluble E dimers [E85]) successfully immunized and protected macaques against dengue virus, while antivector antibodies did not interfere with a booster immunization. Second, compared to prME-VRP, E85-VRP induced neutralizing antibodies faster, to higher titers, and with improved protective efficacy. Third, this study is the first to map antigenic domains and specificities targeted by vaccination versus natural infection, revealing that, unlike prME-VRP and live virus, E85-VRP induced only serotype-specific antibodies, which predominantly targeted EDIII, suggesting a protective mechanism different from that induced by live virus and possibly live attenuated vaccines. Fourth, a tetravalent E85-VRP dengue vaccine induced a simultaneous and protective response to all 4 serotypes after 2 doses given 6 weeks apart. Balanced responses and protection in macaques provided further support for exploring the immunogenicity and safety of this vaccine candidate in humans.

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Section: Discussionmentioning

confidence: 99%

An Alphavirus Vector-Based Tetravalent Dengue Vaccine Induces a Rapid and Protective Immune Response in Macaques That Differs Qualitatively from Immunity Induced by Live Virus Infection

White

Sariol

Mattocks

et al. 2013

J Virol

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“…The strategy used for producing JE VLPs in our recent study would be applicable to other flaviviruses including dengue 6,33 and West Nile viruses. Further developments of an optimal bioreactor system for the long-term, high-density culture of recombinant insect cells would allow the large-scale production of VLPs and improve their productivity.…”

mentioning

confidence: 99%

Production of Japanese encephalitis virus-like particles in insect cells

Yamaji

Konishi

2013

Bioengineered

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“…For other virus systems, such as HIV or influenza, VLPs expressing the envelope glycoproteins are more effective at eliciting high titer neutralizing antibodies than soluble versions [31,[38][39][40][41][42][43][44][45]. Genomeless viral particle vaccines, unlike single proteins, can bind appropriate surface receptors and enter cells in a manner similar to true virus infection [45].…”

Section: Discussionmentioning

confidence: 99%

COBRA-Based Dengue Tetravalent Vaccine Elicits Neutralizing Antibodies Against All Four Dengue Serotypes

2014

J Vaccine immunotechnol

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Dengue virus (DENV) is the most common arthropod-borne infection in the world. The co-circulation of four serotypes, complex pathogenesis, and potential for antibody-enhanced disease has made vaccine development efforts difficult.To ensure protection and minimize vaccine-related disease augmentation, a DENV vaccine must provide equivalent immunity to all four serotypes. Four different vaccine formulations were evaluated for efficacy and utility. DNA plasmid based and purified subviral particles (SVP) vaccines were designed using prototype sequences as well as consensus algorithms known as computationally-optimized broadly reactive antigen (COBRA). These vaccines were formulated against each individual serotype, as well as tetravalent mixtures and used to inoculate mice. All monovalent vaccines elicited neutralizing antibodies against each of their specific homologous virus. In contrast, only purified versions of tetravalent subviral particle (SVP) elicited high levels of neutralizing antibodies against all four serotypes. All Dengue COBRA VLP vaccines elicit a broadly reactive immune response against all four subtypes of dengue virus. A non-infectious SVP vaccine that induces immune protection against the four DENV serotypes could provide a safer alternative candidate to live attenuated viruses.

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Evaluation of Extracellular Subviral Particles of Dengue Virus Type 2 and Japanese Encephalitis Virus Produced bySpodoptera frugiperdaCells for Use as Vaccine and Diagnostic Antigens

Cited by 36 publications

References 52 publications

An Alphavirus Vector-Based Tetravalent Dengue Vaccine Induces a Rapid and Protective Immune Response in Macaques That Differs Qualitatively from Immunity Induced by Live Virus Infection

An Alphavirus Vector-Based Tetravalent Dengue Vaccine Induces a Rapid and Protective Immune Response in Macaques That Differs Qualitatively from Immunity Induced by Live Virus Infection

Production of Japanese encephalitis virus-like particles in insect cells

COBRA-Based Dengue Tetravalent Vaccine Elicits Neutralizing Antibodies Against All Four Dengue Serotypes

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