Evaluation of Extractants for Determination of Micronutrients in Soils of South Gujarat, India

Abstract

An experiment was conducted at Department of Soil Science and Agricultural Chemistry, Navsari Agricultural University during the year 2018-19. The methods of micronutrients determination were (i) extracted through diethylene triamine penta acetic acid (DTPA) (ii) extracted through ammonium bicarbonate- diethylene triamine penta acetic acid (AB-DTPA) (iii) extracted through Neubauer. The methods of micronutrients were evaluated through method validation parameters like linearity, sensitivity of instruments, precision and predictability. The available micronutrients determined from the sample soils were correlated with soil physico-chemical properties to know the relationship of these properties with available micronutrients. The linearity study of the series of micronutrients standard were measured through different methods of micronutrient determination. These measured values showed linear relationship with reference values. The coefficient of determination (R2) for all the methods was > 0.90. The precision was determined by relative standard deviation (RSD) (%) of different micronutrient methods which were in the range of 1.834 to 19.11 %. All the studied methods of copper, iron, zinc and manganese determination, AB-DTPA extractant method has highly significant and positive correlation with nutrient uptake by wheat on Microwave Plasma Atomic Emission Spectroscopy (MP-AES) instrument. Organic C and sand content of soil have positive correlation with micronutrients availability. While pH, EC, CEC, CaCO3, silt and clay content of soil have negative correlation with available micronutrients.