Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes

Abstract

Gout is a metabolic disorder due to the deposition of uric acid crystals within articular or periarticular tissues. Uricase (urate oxidase) catalyzes the oxidation of less water soluble uric acid (7 mg/dl) to a compound allantoin which is more water soluble(11g/L at 40°C) resulting into the ease of excretion of uric acid. The objective of the work was to develop a new method for screening of microbes for uricase production and estimation of uricase thereof. This was achieved by utilizing the fact that uric acid dissolves on being acted upon by uricase. The proposed method is a novel, inexpensive, simple and sensitive technique for screening and estimation of uricase. Biomass and uricase production at different stages of microbial growth curve for the uricase producing microbe was studied. Effects of different medium components affecting uricase production by microbes were studied using Placket Burman statistical design. Addition of uric acid in the nutrient medium was found to be effective in increasing the uricase production by microbes growing in the medium.

Mathematical modeling for bioprocess optimization of a protein drug, uricase, production by Aspergillus welwitschiae strain 1–4

“…The extracellular uricase producing microorganisms convert the suspended insoluble, white crystals of uric acid in the medium used for agar plate assay method to water soluble allantoin, thus producing a clear zone around the colonies. The produced clear zone diameter is directly linked to the extracellular uricase production, meaning a larger clear zone diameter indicated a greater activity of extracellular uricase 25 . The formation of clear zone around the fungal colony of Aspergillus sp.…”