KEYWORD: Dou River Basin; water chemistry; riverbank; human activity ABSTRACT: It is important to study the temporal and spatial distribution of water chemistry for the regional water resources management. In this study, in order to obtain the water chemistry distribution of Dou River Basin, collection and test works have been done for surface water and groundwater, and the results show that, the surface water showed less influence of evaporation effect, but the effect of the sewage makes part of surface water classed from the HCO3 type to SO4 type water, the groundwater far from the riverbank, Ca2+ and HCO3-, NO3-are the main contributors for the TDS increasing in groundwater, which means that, the groundwater far from riverbank affected by the human activities, and the groundwater near riverbank affected the dissolution of limestone and gypsum after the infiltration, and the evaporation and human activities also make important controlling effects on groundwater chemistry.