Evaluation of faecal preservation and staining methods in the diagnosis of acute amoebiasis and giardiasis.

Shetty, Nandini P.; Prabhu, T

doi:10.1136/jcp.41.6.694

Cited by 17 publications

(12 citation statements)

References 4 publications

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“…The cysts are ovoid or ellipsoid and measure 11-15 mm in diameter. Trophozoites are approximately the same size, with 2 anteriorly placed nuclei and 8 flagella best visualized by staining with trichrome or with the iron hematoxylin method [39,40].…”

Section: New Diagnostic Methods For Potentially Treatable Causes Of Pmentioning

confidence: 99%

Global Impact of Diarrheal Diseases That Are Sampled by Travelers: The Rest of the Hippopotamus

Guerrant

Oriá

Bushen

et al. 2005

Clinical Infectious Diseases

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Travelers who experience diarrhea (i.e., "turista") are exposed to the same pathogens and illnesses that pose some of the greatest threats to life and development among malnourished children in developing areas around the world, where inadequate water and poor sanitation remain. This article focuses on new findings about the impact, diagnosis, and control of these illnesses and the genetic predispositions of persons who acquire them. Despite the reductions in mortality due to dehydrating diarrhea, the morbidity associated with diarrheal illnesses continues unabated. Furthermore, we increasingly recognize the lasting detrimental effects of enteric infections that occur during early childhood on later physical and cognitive development and, in patients with acquired immunodeficiency syndrome, on the absorption of antiretroviral drugs. Genetic predispositions to inflammation and potential protection associated with such alleles as ApoE4, which are not suspected of being involved in diarrhea, remind us of how much we have to learn about the effect and interactions of enteric tropical infectious diseases with regard to our host genome. New diagnostic methods hold promise for improved recognition and, hopefully, control of enteric infections worldwide.

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Section: New Diagnostic Methods For Potentially Treatable Causes Of Pmentioning

confidence: 99%

Global Impact of Diarrheal Diseases That Are Sampled by Travelers: The Rest of the Hippopotamus

Guerrant

Oriá

Bushen

et al. 2005

Clinical Infectious Diseases

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show abstract

“…dispar (63, 64, 138a, 164, 171, 229). Shetty and Prabhu found that D'Antoni's iodine was much better than saline or buffered methylene blue for detection of E. histolytica cysts while saline and buffered methylene blue were equally good for detection of E. histolytica trophozoites (206). There are several factors that adversely affect the results of microscopy.…”

Section: Microscopymentioning

confidence: 99%

Laboratory Diagnosis of Amebiasis

2003

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The detection of Entamoeba histolytica, the causative agent of amebiasis, is an important goal of the clinical microbiology laboratory. To assess the scope of E. histolytica infection, it is necessary to utilize accurate diagnostic tools. As more is discovered about the molecular and cell biology of E. histolytica, there is great potential for further understanding the pathogenesis of amebiasis. Molecular biology-based diagnosis may become the technique of choice in the future because establishment of these protozoa in culture is still not a routine clinical laboratory process. In all cases, combination of serologic tests with detection of the parasite (by antigen detection or PCR) offers the best approach to diagnosis, while PCR techniques remain impractical in many developing country settings. The detection of amebic markers in serum in patients with amebic colitis and liver abscess appears promising but is still only a research tool. On the other hand, stool antigen detection tests offer a practical, sensitive, and specific way for the clinical laboratory to detect intestinal E. histolytica. All the current tests suffer from the fact that the antigens detected are denatured by fixation of the stool specimen, limiting testing to fresh or frozen samples

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“…which do not float well because of their high specific gravity. Nevertheless, according to some authors they should also be used especially for identification of protozoan parasites [ 16 ],[ 28 ] or serve as ‘all-round-method’ for all parasite stages [ 24 ],[ 29 ]. Another recently established method (FLOTAC) has been shown to be a sensitive technique for diagnosis of parasitic infections in reptiles [ 30 ] but requires a specially developed apparatus.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of gastrointestinal parasites in reptiles: comparison of two coprological methods

et al. 2014

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BackgroundExotic reptiles have become increasingly common domestic pets worldwide and are well known to be carriers of different parasites including some with zoonotic potential. The need of accurate diagnosis of gastrointestinal endoparasite infections in domestic reptiles is therefore essential, not only for the well-being of captive reptiles but also for the owners. Here, two different approaches for the detection of parasite stages in reptile faeces were compared: a combination of native and iodine stained direct smears together with a flotation technique (CNF) versus the standard SAF-method.ResultsA total of 59 different reptile faeces (20 lizards, 22 snakes, 17 tortoises) were coprologically analyzed by the two methods for the presence of endoparasites. Analyzed reptile faecal samples contained a broad spectrum of parasites (total occurence 93.2%, n = 55) including different species of nematodes (55.9%, n = 33), trematodes (15.3%, n = 9), pentastomids (3.4%, n = 2) and protozoans (47.5%, n = 28). Associations between the performances of both methods to detect selected single parasite stages or groups of such were evaluated by Fisher's exact test and marginal homogeneity was tested by the McNemar test. In 88.1% of all examined samples (n = 52, 95% confidence interval [CI] = 77.1 - 95.1%) the two diagnostic methods rendered differing results, and the McNemar test for paired observations showed highly significant differences of the detection frequency (P < 0.0001).ConclusionThe combination of direct smears/flotation proved superior in the detection of flagellates trophozoites, coccidian oocysts and nematode eggs, especially those of oxyurids. SAF-technique was superior in detecting larval stages and trematode eggs, but this advantage failed to be statistically significant (P = 0.13). Therefore, CNF is the recommended method for routine faecal examination of captive reptiles while the SAF-technique is advisable as additional measure particularly for wild caught animals and individuals which are to be introduced into captive collections.

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Evaluation of faecal preservation and staining methods in the diagnosis of acute amoebiasis and giardiasis.

Cited by 17 publications

References 4 publications

Global Impact of Diarrheal Diseases That Are Sampled by Travelers: The Rest of the Hippopotamus

Global Impact of Diarrheal Diseases That Are Sampled by Travelers: The Rest of the Hippopotamus

Laboratory Diagnosis of Amebiasis

Diagnosis of gastrointestinal parasites in reptiles: comparison of two coprological methods

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