Evaluation of Fe employing X-Ray Fluorescence Methodology (XRF) in mice skin during acute phase of experimental infection with Trypanosoma cruzi

Estevam, Marcelo; Malvezi, Aparecida Donizette; Borges, C. L.; Pinge‐Filho, Phileno; Appoloni, Carlos Roberto

doi:10.1063/1.2710640

Cited by 3 publications

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“…Tests were also designed to validate the system with iron concentrations of 30 ppm, 70 ppm, and 90 ppm. The uncertainty of the simulation values was T5 ppm of iron due to the chemical balance instability and production errors (Estevam and Appoloni 2007).…”

Section: Resultsmentioning

confidence: 99%

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Use of Portable X-ray Fluorescence (PXRF) In Vivo as an Alternative Technique for the Assessment of Iron Levels in Patients With Thalassemia and Hemochromatosis

Estevam¹,

Appoloni²

2013

Health Physics

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This work investigated the viability of the portable x-ray fluorescence (PXRF) technique as a means of measuring iron levels in patients suffering from thalassemia major (beta-thalassemia) and hereditary hemochromatosis (HH or Haemochromatosis) or other iron-overload conditions. Measurements were conducted at the University Hospital Blood Center and in the Laboratory of Applied Nuclear Physics at the State University of Londrina, Brazil. One thalassemia major patient and four healthy people were grouped. A PXRF system consisting of a Pu x-ray source and a SiPIN diode detector was used for measurements in vivo on the hand of each patient. The measuring system was calibrated with phantoms of aqueous solution doped with 15 to 150 ppm of iron. The duration of each measurement was 50 s. The detection limit (LLD) reached for iron was 13 ppm. The radiation dose on the skin was 10 mSv. The thalassemia patient presented 74 ± 6 ppm of iron, whereas the healthy people presented an average of 53 ± 5 ppm of iron. The results are in accord with the literature, which shows iron levels in the skin to be between 15 - 60 ppm for healthy people and between 70 - 150 ppm for thalassemia major patients. This work concluded that it is viable to apply the XRF methodology to follow thalassemia major and HH patients.

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Section: Resultsmentioning

confidence: 99%

“…Some studies show that iron overload in the organism is proportional to the iron concentration in the skin (Estevam and Appoloni 2009;Estevam et al 2007;Gorodetsky et al 1990). Estevam and Appoloni (2009) are currently testing the possibility of ferritin levels' correlation with measures of in vivo x-ray fluorescence (XRF).…”

Section: Introductionmentioning

confidence: 98%

Use of Portable X-ray Fluorescence (PXRF) In Vivo as an Alternative Technique for the Assessment of Iron Levels in Patients With Thalassemia and Hemochromatosis

Estevam¹,

Appoloni²

2013

Health Physics

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“…Alguns estudos informam que a sobrecarga férrea do organismo é proporcional à concentração de ferro na pele. 1,2,3 Apresentamos neste trabalho medidas in vivo na pele da mão de quatro pessoas sadias e um portador de talassemia maior através da técnica de fluorescência de raios X (XRF), técnica precisa, rápida, não invasiva e capaz de repetições regulares.…”

Section: Introductionunclassified

Uso da fluorescência de raios X portátil (XRF) in vivo como técnica alternativa para acompanhamento dos níveis de ferro em pacientes com sobrecarga de ferro

Estevam¹,

Appoloni²

2009

Rev. Bras. Hematol. Hemoter.

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Trypanosoma cruzi: in vivoevaluation of iron in skin employing X-ray fluorescence (XRF) in mouse strains that differ in their susceptibility to infection

Estevam

Appoloni

Malvezi

et al. 2012

FEMS Immunol Med Microbiol

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Trypanosoma cruzi, the causative agent of Chagas' disease (CD), is a substantial public health concern in Latin America. Laboratory mice inoculated with T. cruzi have served as important animal models of acute CD. Host hypoferremic responses occur during T. cruzi infection; therefore, it has been hypothesized that T. cruzi requires iron for optimal growth in host cells and, unlike extracellular pathogens, may benefit from host hypoferremic responses. Recent technological improvements of X-ray fluorescence are useful for diagnostics or monitoring in biomedical applications. The goal of our study was to determine whether the iron availabilities in Swiss and C57BL/6 mice differ during the acute phase of T. cruzi infection and whether the availability correlates with oxidative stress in the susceptible and resistant phenotypes identified in these mice. Our results showed that the decrease in iron levels in the skin of resistant infected mice correlated with the increase in oxidative stress associated with anemia and the reduction in parasite burden.

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Evaluation of Fe employing X-Ray Fluorescence Methodology (XRF) in mice skin during acute phase of experimental infection with Trypanosoma cruzi

Cited by 3 publications

References 0 publications

Use of Portable X-ray Fluorescence (PXRF) In Vivo as an Alternative Technique for the Assessment of Iron Levels in Patients With Thalassemia and Hemochromatosis

Use of Portable X-ray Fluorescence (PXRF) In Vivo as an Alternative Technique for the Assessment of Iron Levels in Patients With Thalassemia and Hemochromatosis

Uso da fluorescência de raios X portátil (XRF) in vivo como técnica alternativa para acompanhamento dos níveis de ferro em pacientes com sobrecarga de ferro

Trypanosoma cruzi: in vivoevaluation of iron in skin employing X-ray fluorescence (XRF) in mouse strains that differ in their susceptibility to infection

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