Evaluation of fish protein hydrolysates in juvenile African catfish (Clarias gariepinus) diets

Swanepoel, Johannes Christoffel; Goosen, Neill J.

doi:10.1016/j.aquaculture.2018.06.084

Cited by 50 publications

(26 citation statements)

References 61 publications

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“…Glycine, glutamic acid, aspartic acid, and alanine were the predominant amino acids in turbot FPHs with remarkable content in the essential ones: TEAA/TAA values were higher than 28% (Table 3). Considering the concentrations of total and soluble proteins and the profile of amino acids along with the values of digestibility, valuable applications of turbot hydrolysates can be envisaged such as an ingredient in human food supplements and animal feed [10,35]. Further trials should be conducted to assess these potentialities.…”

Section: Production and Chemical Composition Of Turbot Fphsmentioning

confidence: 99%

“…[7][8][9]. All these benefits make fish hydrolysates a valuable and easily digestible protein supplement, with high nutritional properties and interesting applications as an ingredient for human and animal functional foods [5,10,11]. From the 1940s, the number of fish species, types of fishery wastes, and proteolytic enzymes (both endo-and exogenous) studied for FPHs production has been extensive [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Production, Characterization, and Bioactivity of Fish Protein Hydrolysates from Aquaculture Turbot (Scophthalmus maximus) Wastes

et al. 2020

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The valorization of wastes generated in the processing of farmed fish is currently an issue of extreme relevance for the industry, aiming to accomplish the objectives of circular bioeconomy. In the present report, turbot (Scophthalmus maximus) by-products were subjected to Alcalase hydrolysis under the optimal conditions initially defined by response surface methodology. All the fish protein hydrolysates (FPHs) showed a high yield of digestion (>83%), very remarkable degrees of hydrolysis (30–37%), high content of soluble protein (>62 g/L), an excellent profile of amino acids, and almost total in vitro digestibility (higher than 92%). Antioxidant and antihypertensive activities were analyzed in all cases, viscera hydrolysates being the most active. The range of average molecular weights (Mw) of turbot hydrolysates varied from 1200 to 1669 Da, and peptide size distribution showed that the hydrolysate of viscera had the highest content of peptides above 1000 Da and below 200 Da.

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Section: Production and Chemical Composition Of Turbot Fphsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production, Characterization, and Bioactivity of Fish Protein Hydrolysates from Aquaculture Turbot (Scophthalmus maximus) Wastes

et al. 2020

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“…The functional capacity of FPHs in terms of antihypertensive, antioxidant, antiproliferative, antimicrobial, etc., in vitro bioactivities, is one of the most valuable properties of these bioproduction [11][12][13][14]. Additionally, since hydrolysates are composed of soluble proteins, peptides, and free amino acids, they are an excellent ingredient of aquaculture feeds and pet-food diets in substitution of the conventional fishmeals, improving generally the effectiveness of feeds and diets to support fish and animal healthy growths [15][16][17][18].However, complete studies of production of enzymatic hydrolysates from salmonid wastes including optimization of proteolysis conditions, analysis of kinetic hydrolysis, chemical characterization of all products obtained, bioactivities, and peptides size distribution are practically non-existent. Therefore, the aims of this work are (1) optimization of the experimental conditions to produce FPHs, using Alcalase, of salmonid by-products (heads of salmon and rainbow trout) by response surface methodology (RSM), (2) mathematical analysis of hydrolysis kinetics by Weibull equation, (3) chemical characterization of products obtained from salmonid hydrolysis, (4) identification of average molecular weights and peptide size distribution of the hydrolysates, and (5) determination of two bioactives (antioxidant and antihypertensive) from FPHs.Mar.…”

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confidence: 99%

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confidence: 99%

Valorization of Aquaculture By-Products of Salmonids to Produce Enzymatic Hydrolysates: Process Optimization, Chemical Characterization and Evaluation of Bioactives

et al. 2019

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In the present manuscript, various by-products (heads, trimmings, and frames) generated from salmonids (rainbow trout and salmon) processing were evaluated as substrates for the production of fish protein hydrolysates (FPHs), potentially adequate as protein ingredients of aquaculture feeds. Initially, enzymatic conditions of hydrolysis were optimized using second order rotatable designs and multivariable statistical analysis. The optimal conditions for the Alcalase hydrolysis of heads were 0.1% (v/w) of enzyme concentration, pH 8.27, 56.2 • C, ratio (Solid:Liquid = 1:1), 3 h of hydrolysis, and agitation of 200 rpm for rainbow trout and 0.2% (v/w) of enzyme, pH 8.98, 64.2 • C, 200 rpm, 3 h of hydrolysis, and S:L = 1:1 for salmon. These conditions obtained at 100 mL-reactor scale were then validated at 5L-reactor scale. The hydrolytic capacity of Alcalase and the protein quality of FPHs were excellent in terms of digestion of wastes (V dig > 84%), high degrees of hydrolysis (H m > 30%), high concentration of soluble protein (Prs > 48 g/L), good balance of amino acids, and almost full in vitro digestibility (Dig > 93%). Fish oils were recovered from wastes jointly with FPHs and bioactive properties of hydrolysates (antioxidant and antihypertensive) were also determined. The salmon FPHs from trimmings + frames (TF) showed the higher protein content in comparison to the rest of FPHs from salmonids. Average molecular weights of salmonid-FPHs ranged from 1.4 to 2.0 kDa and the peptide sizes distribution indicated that hydrolysates of rainbow trout heads and salmon TF led to the highest percentages of small peptides (0-500 Da). 676 2 of 15 and volume terms in the European fish farming system. More than 1.78 MTm of both were produced in 2016 generating more than € 15 billion [1]. Although in southern Europe the most common way to market salmonids is as complete individuals, in the rest of the continent the tendency is to market them in the form of fillets (fresh, frozen, cured, etc.) or other presentations that require deheading, gutting, and filleting steps. Thus, huge amounts of by-products (about 35-45% of the total weight of salmonids) are generated in processing plants, mainly heads, trimmings, viscera, and frames that have to be managed efficiently to reduce environmental health problems and to improve the sustainability of such farming productions [2,3].The most habitual utilization of salmonid wastes is based on the development of acid silage [3], fertilizers, or the joint production of fishmeal and oils [4,5]. In the first case, the silage is a low-added value product with limited applications [1]. In the last one, oil and fishmeal are not very sustainable productions when the fishmeal equipments are far from fishing ports or aquaculture plants and close to villages and cities due to the environmental impact (odors, air pollution, water consumption, etc.) that this production generates. This process leads to the coagulation of the protein and its separation from the oil but the level of valorization achieved by b...

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“…Many studies have shown considerable success in partial or total replacement of dietary FM with plant protein (PP) sources for various marine fish species during the on-growing phase (Hernández et al, 2007;Salze et al, 2010;Moxley et al, 2014;Yaghoubi et al, 2016;Lazzarotto et al, 2018;Kotzamanis et al, 2018 among others). However, there exist few studies addressing this issue at younger stages of development and how these feeding strategies affect their performance, digestive capacities and nutritional condition (El-Saidy and Gaber, 2003;Enyidi and Mgbenka, 2015;Gisbert et al, 2016;Swanepoel and Goosen, 2018).…”

Section: Introductionmentioning

confidence: 99%

Weaning European glass eels (Anguilla anguilla) with plant protein-based diets and its effects on intestinal maturation

Gisbert

Mozanzadeh

2019

Comparative Biochemistry and Physiology Part A: Molecular &

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Weaning glass eels with compound diets (36% proteins, 16% lipids) differing in their fishmeal (FM) level (50, 75 and 100% FM replaced by a blend of plant proteins, PP) was compared to a group fed cod roe. Weaning lasted for 20 days and then, eels were fed compound diets for 70 days, whereas the other group was only fed cod roe (90 days). Diets were tested with four replicates and evaluated in terms of growth, survival, glass eels metamorphosis into elvers, oxidative stress status and activity of digestive enzymes. Although glass eels are fed with fish roe and progressively weaned onto compound diets, results revealed that this strategy should not be prolonged for a long time, since 2 feeding glass eels with cod roe for 90 days negatively affected their growth (2 times lower than fish fed compound diets), delayed their metamorphosis, as well as the maturation of their digestive function as the ratio of alkaline phosphatase and leucine-alanine peptidase indicated. Weaning glass eels onto compound diets differing in their FM levels did not affect their growth, metamorphic stage nor the activity of pancreatic enzymes (total alkaline proteases, trypsin, bile salt-activated lipase and α-amylase), although 75% FM replacement by PP sources delayed the level of intestinal maturation in eels. In comparison to glass eels fed the 100% FM diet, survival was negatively affected in groups fed diets with 50 and 75% FM replacement by PP ingredients, which indicated that further improvement is needed in diet formulation for this stage of development.

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Evaluation of fish protein hydrolysates in juvenile African catfish (Clarias gariepinus) diets

Cited by 50 publications

References 61 publications

Production, Characterization, and Bioactivity of Fish Protein Hydrolysates from Aquaculture Turbot (Scophthalmus maximus) Wastes

Production, Characterization, and Bioactivity of Fish Protein Hydrolysates from Aquaculture Turbot (Scophthalmus maximus) Wastes

Valorization of Aquaculture By-Products of Salmonids to Produce Enzymatic Hydrolysates: Process Optimization, Chemical Characterization and Evaluation of Bioactives

Weaning European glass eels (Anguilla anguilla) with plant protein-based diets and its effects on intestinal maturation

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