Evaluation of five DNA extraction systems for recovery of DNA from bone

Iyavoo, Sasitaran; Hadi, Sibte; Goodwin, William

doi:10.1016/j.fsigss.2013.10.090

Cited by 13 publications

(12 citation statements)

References 6 publications

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“…In a study performed on fresh porcine bone by Iyavoo et al , it was proposed that organic phenol–chloroform produced higher DNA yields when compared to ChargeSwitch® gDNA Plant Kit and DNeasy Blood and Tissue Kit, respectively. Because this study focused on environmentally challenged samples rather than fresh bone, it is important to note that, in contrast to Iyavoo et al's results, ChargeSwitch® gDNA Plant Kit performed better, suggesting that the magnetic bead technology may be better for waterlogged bone samples.…”

Section: Discussionmentioning

confidence: 99%

“…Fredericks et al also reported that the success of DNA extraction varies with both bone type and elongation of submersion. In addition, Iyavoo et al compared DNA extracted from femur and rib bones using five different methods. For each method, they reported a significant difference between the bones, and for each method, tested rib samples had higher DNA yield.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of DNA Extraction Methods from Waterlogged Bones: A Pilot Study,

Cartozzo

Singh

Boone

et al. 2018

Journal of Forensic Sciences

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When deaths occur in water, soft tissue decomposes after a temperature-dependent period, making DNA identification dependent on bone. This study examined the effects of water on bone DNA purity and quantity, and determined the best of three extraction methods for isolating DNA. The organic phenol-chloroform method consistently extracted DNA approximating the accepted 260/280 purity value (~1.8); ChargeSwitch® gDNA Plant Kit and DNeasy Blood and Tissue Kit produced fair and unacceptable values, respectively. The purity value for humerus and rib samples was consistent across accumulated degree days (ADD). Significant differences in quantification among extraction methods and between bone types were identified. Ribs and ChargeSwitch® gDNA Plant Kit samples produced the lowest mean Ct values of the bone types and the extraction methods, respectively. Therefore, this study proposes that magnetic bead technology extraction methods and ribs be considered when processing bones that have been submerged in water for any length of time.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of DNA Extraction Methods from Waterlogged Bones: A Pilot Study,

Cartozzo

Singh

Boone

et al. 2018

Journal of Forensic Sciences

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“…To all the 24 samples (decalcified and non-decalcified) a volume of 400 μl of digestion buffer (10 mM Tris-HCl, 10 mM EDTA pH 8, 50 mM NaCl and 2% (w/v) SDS) and 15 μl of proteinase K (20 mg/ml) (Qiagen ® , UK) were added and placed in the incubator at 56°C overnight. After incubation, the phenol-chloroform extraction was carried out and the final volume was standardized at 100 μl for comparison [3].…”

Section: Dna Extractionmentioning

confidence: 99%

Evaluation of decalcification for recovery of DNA from bone

Iyavoo

Hadi

Goodwin

2017

Forensic Science International: Genetics Supplement Series

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The effect of decalcification on pulverised bone samples was studied to evaluate its value when processing reasonably good quality samples. The decalcified and non-decalcified bone samples were extracted using phenolchloroform extraction method, quantitated with GoTaq ® qPCR Master Mix (Promega ® , UK) using Applied Biosystems ® 7500 Real-Time PCR System (Thermo Fisher Scientific ® , UK) and amplified using an in-house amplification multiplex. The results showed that the decalcification of bone samples is not a necessary when processing good quality samples and higher DNA yields were obtained without the decalcification process. The electropherograms produced from the extracted DNA samples without decalcification were good quality and displayed no PCR inhibition.

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“…The investigations was begin to identify the remains and determine the biological relation between the buried fetus and the two suspects. In order to achieve the best possible results, many techniques have been tested and published [1][2][3]. The organic method using phenol-chloroform-isoamyl alcohol…”

Section: Case Reportmentioning

confidence: 99%

“…‫العظام‬ ‫من‬ ‫النووي‬ ‫احلم�ض‬ ‫ال�ستخال�ض‬ ‫منا�سبة‬ ‫طريقة‬ ‫حالة‬ ‫درا�سة‬ ‫اجلنائية:‬ ‫التطبيقات‬ ‫يف‬ ‫ال�ستخدامه‬ ‫امل�ستخل�ض‬ ‫اكت�شاف‬ ‫فقبل‬ ‫ثابت.‬ ‫ب�شكل‬ ‫الب�شرية‬ ‫الهوية‬ ‫حتديد‬ ‫تقنيات‬ ‫تتطور‬ ‫اال�شنان‬ ‫وطب‬ ‫االن�شان)‬ ‫(علم‬ ‫أنرثوبولوجي‬ ‫اال‬ ‫علم‬ ‫كان‬ ‫الوراثية‬ ‫الب�شمة‬ ‫احلقبة‬ ‫مع‬ ‫الب�شرية.‬ ‫الهوية‬ ‫لتحقيق‬ ‫امل�شتخدمة‬ ‫التطبيقات‬ ‫أكرث‬ ‫ا‬ ‫من‬ ‫احلم�ض‬ ‫حتليل‬ ‫تقنية‬ ‫يف‬ ‫حدثت‬ ‫التي‬ ‫والثورة‬ ‫اجلزيئية‬ ‫أحياء‬ ‫لل‬ ‫اجلديدة‬ ‫الوراثية‬ ‫ال�شمات‬ ‫حتديد‬ ‫أ�شبح‬ ‫ا‬ ‫املت�شل�شل،‬ ‫البلمرة‬ ‫تفاعل‬ ‫وكذلك‬ ‫النووي‬ ‫اجلنائي.‬ ‫الب�شرية‬ ‫الهوية‬ ‫حتديد‬ ‫عملية‬ ‫أ�شا�ض‬ ‫ا‬ ‫ميكن‬ ‫التي‬ ‫العينات‬ ‫نوع‬ ‫أهمها‬ ‫ا‬ ‫من‬ ‫حتديات،‬ ‫عدة‬ ‫املجال‬ ‫هذا‬ ‫تواجه‬ ‫التحلل‬ ‫من‬ ‫الق�شوى‬ ‫احلاالت‬ ‫يف‬ ‫انه‬ ‫حيث‬ ‫وحالتها.‬ ‫لل�شتعراف‬ ‫تتوفر‬ ‫أن‬ ‫ا‬of its reagents has reduced its routine application in forensic laboratories. New techniques were then developed: the silica-based methods have been used for many years due to their easiness, efficiency to reduce inhibitors, and the ability of automation[3].…”

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