Evaluation of Four Methods for the Diagnosis of Respiratory Syncytial Virus Infection in Older Adults

Falsey, Ann R.; McCann, Robert M.; Hall, William J.; Criddle, Mary M.

doi:10.1111/j.1532-5415.1996.tb05641.x

Cited by 72 publications

(53 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…RSV is the main cause of childhood viral ARI [28] and has been detected among other respiratory viruses, mainly influenza A, rhinovirus, coronaviruses and parainfluenza viruses, in exacerbations of COPD [29][30][31][32]. For older adults it has been shown that the diagnosis of RSV with less sensitive tests can be difficult and are not always reliable, leading to the complementary use of different methods for RSV detection [33,34]. The main advantages of the RSV-A specific qPCR assay are high specificity and sensitivity as well as its exact quantification and reproducibility.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a quantitative real-time PCR for the detection of respiratory syncytial virus in pulmonary diseases

Borg¹,

Rohde²,

Löseke³

et al. 2003

Eur Respir J

102

View full text Add to dashboard Cite

Respiratory syncytial virus (RSV) is known to cause acute lower respiratory tract infections (ARI) in young children and is involved in exacerbation of chronic obstructive pulmonary disease (COPD) in adults. The role of RSV in stable COPD and the viral load in different respiratory diseases has not been investigated to date.The present authors established and evaluated a quantitative TaqMan1 real-time polymerase chain reaction assay specific for RSV subgroup A. Absolute quantification for the determination of viral load input was achieved using a control plasmid. The assay allowed for a quantification over aw6-log range and a detection limit ofv10 RSV copies per reaction mixture.The assay was 30 times more sensitive than conventional nested polymerase chain reaction assays. Interassay SD was 1.3 and coefficient of variation 4.7% on average. Clinical specimens from infants with ARI (n=62) and elderly adults with COPD (n=125) were compared for viral loads. A total of 47% RSV-positive samples were found in the ARI study group and 28% in the COPD study group. The viral load of both study groups was found to differ significantly. In the ARI study group the viral load was increased almost 2000-fold, suggesting acute infection in this group and former or latent infection in the COPD group.Respiratory syncytial virus-A specific TaqMan1 real-time polymerase chain reaction assay is a sensitive and rapid method for the determination of viral load in clinical samples. It enables differential statements concerning the involvement of respiratory syncytial virus in acute lower respiratory tract infections and chronic obstructive pulmonary disease to be achieved. Eur Respir J 2003; 21: 944-951.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of a quantitative real-time PCR for the detection of respiratory syncytial virus in pulmonary diseases

Borg¹,

Rohde²,

Löseke³

et al. 2003

Eur Respir J

102

View full text Add to dashboard Cite

show abstract

“…However, elderly or immunocompromised adults may develop severe RSV infection (4,5,23). In contrast to children, diagnosis in adults is often difficult due to the insensitivity of viral culture (7). This is likely due to thermolability of the virus and low titers of virus shed by adults and may also be due to the presence of preexisting nasal antibody in clinical specimens resulting in in vitro neutralization.…”

mentioning

confidence: 97%

Comparison of Quantitative Reverse Transcription-PCR to Viral Culture for Assessment of Respiratory Syncytial Virus Shedding

et al. 2003

Self Cite

View full text Add to dashboard Cite

Respiratory syncytial virus (RSV) has recently been recognized as a serious pathogen in elderly and immunocompromised adults. Diagnosis of acute infection in adults is often difficult due to the insensitivity of viral culture, and reverse transcription-PCR (RT-PCR) is a more sensitive alternative. The relationship of quantitative RT-PCR to viable virus has never been studied for RSV. Therefore, we compared a quantitative real-time RT-PCR with viral culture to assess viral load in adult volunteers challenged with the RSV A2 strain. Twelve of 13 volunteers were infected, and there was a high correlation (r ‫؍‬ 0.84) between quantitative RT-PCR and viral titer by cell culture. However, RT-PCR was more sensitive, with 73 of 169 (43%) samples positive compared to 58 (34%) samples positive by culture. The correlation between the two tests was highest early in the course of viral shedding (r ‫؍‬ 0.91, days 0 to 6), whereas during days 7 to 13, there was more variability (r ‫؍‬ 0.70). All subjects were culture negative by day 11, whereas one subject remained RT-PCR positive on day 12. All subjects were RT-PCR negative at day 28 postinfection. Quantitative RT-PCR has an excellent correlation with viral titers, as measured by culture, and should be a useful tool for future studies addressing viral load and disease pathogenesis.Respiratory syncytial virus (RSV) is a frequent cause of serious lower respiratory tract disease in young children (13). Reinfections occur throughout life and are generally mild in healthy adults. However, elderly or immunocompromised adults may develop severe RSV infection (4,5,23). In contrast to children, diagnosis in adults is often difficult due to the insensitivity of viral culture (7). This is likely due to thermolability of the virus and low titers of virus shed by adults and may also be due to the presence of preexisting nasal antibody in clinical specimens resulting in in vitro neutralization. Thus, investigators have increasingly relied on new sensitive molecular techniques such as reverse transcription PCR (RT-PCR) for the immediate diagnosis of RSV infection in adults (6, 9-11, 14, 30). In addition, the recent development of real-time RT-PCR, in which the concentration of amplification products is monitored as they accumulate during thermal cycling, allows quantification of RNA in specimens (18). The ability to assess the relationship of viral load to severity of illness and determination of the location of viral replication in the respiratory tract would improve the understanding of disease pathogenesis in adults as well as children. Quantitative RT-PCR has been used successfully to monitor disease progression and assess response to therapy for malignant diseases and other viral infections that are difficult to cultivate, such as human immunodeficiency virus and hepatitis C virus (2,12,15,20,29). However, the relationship of RNA copy to viable organisms and clinical illness is less well defined for respiratory pathogens, including RSV (9,16,17,19,22). Therefore, we developed a quanti...

show abstract

“…De las 114 muestras restantes con sospecha de IRV e IFD negativa para virus respiratorios, 17 (14,9%) resultaron positivas para VRS por TR-RPC en tiempo real, todos tipo B. Esto representa un aumento de 121% en la capacidad de diagnóstico de VRS en ese momento. En la Figura 1 se muestra la distribución variable de la positividad de los exámenes (IFD y TR-RPC tiempo real), según la semana epidemiológica (Figura 1A), observándose un paralelismo entre los resultados observados en los pacientes internados con prueba positiva y la detección del virus en la comunidad en las mismas semanas de vigilancia epidemiológica para VRS en Santiago (Figura 1B) 12 .…”

Section: Resultsunclassified

“…Según datos de vigilancia epidemiológica de virus respiratorios de la ciudad de Santiago 12 , durante el período de estudio se registraba una baja progresiva de casos de VRS, posterior a su mayor actividad ocurrida en la semana 29; este comportamiento fue similar al observado en la curva obtenida al graficar las muestras positivas por semana epidemiológica, incluyendo IFD más RPC positivas; este hecho nos ratifica que, entre adultos hospitalizados, se refleja la actividad de VRS presente en la comunidad 2 .…”

Section: Discussionunclassified

Utilidad de la reacción de polimerasa en cadena en tiempo real en el diagnóstico de infecciones por virus respiratorio sincicial en adultos

Serri

et al. 2007

Rev. chil. infectol.

View full text Add to dashboard Cite

Introduction: Viral respiratory infections (VRI) are a frequent cause of morbidity among adult population. Respiratory syncytial virus (RSV) produces 20% of VRI, however diagnosis is limited for a low sensitivity of conventional (FDA and ELISA) tests. Aim: To assess the impact of real time reverse transcriptase-polymerase chain reaction (real time RT-PCR) technique in RSV diagnosis in adult hospitalized patients; to characterize RSV infection among these patients. Patients and Methods: All adults hospitalized in Hospital Clínico Universidad Católica during 8 weeks of winter season, with clinical picture of VRI, and with negative DFA for influenza A and B, parainfluenza 1, 2, 3 and adenovirus were included. Real time RT-PCR was performed from nasopharyngeal sample. Clinical information, general laboratory exams and chest X ray reports were collected. Results: Out of 114 patients with negative DFA, 17 (14.9%) Debe decir: RSV cases were demonstrated using real time RT-PCR. Fever, pharyngeal congestion, cough and bronchial obstruction were present in 80% of patients. Thirty percent of them had a baseline chronic disease and 47% were immunocompromised. One out of 17 patients (6%) required mechanical ventilation. No mortality was observed. Conclusions: Use of RT-PCR allowed increasing detection of RSV infection over 100% among adults with VRI without virological diagnosis with conventional techniques. It is necessary to consider RSV RT-PCR test among patients with clinical picture of VRI during RSV season, with negative virological screening tests.

show abstract

Evaluation of Four Methods for the Diagnosis of Respiratory Syncytial Virus Infection in Older Adults

Abstract: Rapid antigen tests for the diagnosis of RSV in older persons should be used with caution.

Cited by 72 publications

References 10 publications

Evaluation of a quantitative real-time PCR for the detection of respiratory syncytial virus in pulmonary diseases

Evaluation of a quantitative real-time PCR for the detection of respiratory syncytial virus in pulmonary diseases

Comparison of Quantitative Reverse Transcription-PCR to Viral Culture for Assessment of Respiratory Syncytial Virus Shedding

Utilidad de la reacción de polimerasa en cadena en tiempo real en el diagnóstico de infecciones por virus respiratorio sincicial en adultos

Contact Info

Product

Resources

About