Evaluation of Four Strategies for SARS-CoV-2 Detection: Characteristics and Prospects

Abstract

In the study, we reported an evaluation of 4 detection strategies implemented in different scenarios for SARS-CoV-2 detection: single sampling tests, 20-in-1 pooling tests, nucleic acid point-of-care tests, and rapid antigen tests. 3 SARS-CoV-2-inactivated SARS-CoV-2 cell culture supernatants and 5 recombinant SARS-CoV-2 nucleocapsid proteins were used for evaluation. In this analysis, we found that for the WT, Delta, and Omicron supernatants, the lowest concentrations to achieve 100% detection rates of single sampling tests ranged between 1.28 × 10 2 to 1.02 × 10 3 , 1.28 × 10 2 to 4.10 × 10 3 , and 1.28 × 10 2 to 2.05 × 10 3 copies/mL.