Evaluation of fungicides and tobacco cultivar resistance to Rhizoctonia solani incited target spot, damping off and sore shin

Csinos, A. S.; Stephenson, M. G.

doi:10.1016/s0261-2194(99)00037-x

Cited by 23 publications

(21 citation statements)

References 8 publications

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“…The three most tolerant lines were challenged individually with four pathogens of diVerent eukaryotic lineages (Table 1). All pathogen species, isolates, or populations were isolated from or were known to infect tobacco and all inoculations were performed according to standard procedures (Table 1) (Hussey and Barker 1973;Rioger and JeVers 1991;Csinos and Stephenson 1999;Hussey and Janssen 2002;Robertson et al 2004).…”

Section: Disease Resistance Screeningmentioning

confidence: 99%

Gastrodia anti-fungal protein from the orchid Gastrodia elata confers disease resistance to root pathogens in transgenic tobacco

Cox

Layne

Scorza

et al. 2006

Planta

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Diseases of agricultural crops are caused by pathogens from several higher-order phylogenetic lineages including fungi, straminipila, eubacteria, and metazoa. These pathogens are commonly managed with pesticides due to the lack of broad-spectrum host resistance. Gastrodia anti-fungal protein (GAFP; gastrodianin) may provide a level of broad-spectrum resistance due to its documented anti-fungal activity in vitro and structural similarity to insecticidal lectins. We transformed tobacco (Nicotiana tabacum cv. Wisconsin 38) with GAFP-1 and challenged transformants with agriculturally important plant pathogens from several higher-order lineages including Rhizoctonia solani (fungus), Phytophthora nicotianae (straminipile), Ralstonia solanacearum (eubacterium), and Meloidogyne incognita (metazoan). Quantitative real-time PCR and western blotting analysis indicated that GAFP-1 was transcribed and translated in transgenic lines. When challenged by R. solani and P. nicotianae, GAFP-1 expressing lines had reduced symptom development and improved plant vigor compared to non-transformed and empty vector control lines. These lines also exhibited reduced root galling when challenged by M. incognita. Against R. solanacearum expression of GAFP-1 neither conferred resistance, nor exacerbated disease development. These results indicate that heterologous expression of GAFP-1 can confer enhanced resistance to a diverse set of plant pathogens and may be a good candidate gene for the development of transgenic, root-disease-resistant crops.

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Section: Disease Resistance Screeningmentioning

confidence: 99%

Gastrodia anti-fungal protein from the orchid Gastrodia elata confers disease resistance to root pathogens in transgenic tobacco

Cox

Layne

Scorza

et al. 2006

Planta

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“…have been reported (Lucas 1975;Nicoletti and Lahoz 1995;Shew and Melton 1995;Garcia et al 2009;Gurkanli and Ozkoc 2011). In tobacco, these pathogens cause several symptoms including target spot, stem rot and damping-off in transplants and target spot and sore shin in field plants, resulting in crop losses of up to 15 % (Lucas 1975;Shew and Lucas 1991;Csinos and Stephenson 1999;Tarantino 2007;Gonzalez et al 2011). Under wet conditions, especially rainy weather, target spot epidemics may lead to severe losses.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Cárdenas

Galván

Barrera³

et al. 2015

Trop. plant pathol.

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In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse.Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off.

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“…Biological variability among the isolates of AG‐3 is observed as differences in growth rate, severity of symptoms (Chand & Logan, 1983; Carling & Leiner, 1986, 1990a; Bandy et al ., 1988; Anguiz & Martin, 1989; Hill & Anderson, 1989; Bains & Bisht, 1995; Balali et al ., 1995) and sensitivity to fungicides (Carling et al ., 1990; Csinos & Stephenson, 1999; Campion et al ., 2003). However, these studies have included only relatively few isolates of AG‐3, mainly from culture collections.…”

Section: Introductionmentioning

confidence: 99%

Biological diversity of Rhizoctonia solani (AG‐3) in a northern potato‐cultivation environment in Finland

et al. 2007

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A total of 119 isolates of Rhizoctonia were collected from stem canker lesions, stolon and root lesions, hymenia on stems, or from black scurf on tubers of potato plants ( Solanum tuberosum ) in Finland (latitudes 60 -67 ° N). All isolates except three belonged to anastomosis group 3 (AG-3) of R. solani , as determined by phylogenetic analysis of the internal transcribed spacer sequences (ITS1 and ITS2) of ribosomal RNA (rRNA) genes. Sensitivity of the 119 isolates to the fungicide flutolanil was tested in vitro (EC 50 values 0·14-0·75 µ g active ingredient mL − 1 ). The isolates also varied considerably in growth rate (5·1-14·8 mm day). The severity of disease caused by 99 isolates was determined based on the proportion of potato sprouts affected by lesions, discoloration or death, which was c . 1 -60%. Only two isolates that were able to cause severe symptoms showed particularly low sensitivity to the fungicide and rapid growth rate. One isolate each of anastomosis groups AG-2-1 and AG-5 and an unknown, binucleate Rhizoctonia sp. were detected. The AG-5 isolate and the binucleate isolate caused mild symptoms on potato sprouts, whereas the AG-2-1 isolate was not pathogenic. Taken together, AG-3 of R. solani was the predominant causal agent of the stem canker and black scurf diseases of potato in Finland and showed considerable variability in disease severity, fungicide sensitivity and growth rate in vitro .

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Evaluation of fungicides and tobacco cultivar resistance to Rhizoctonia solani incited target spot, damping off and sore shin

Cited by 23 publications

References 8 publications

Gastrodia anti-fungal protein from the orchid Gastrodia elata confers disease resistance to root pathogens in transgenic tobacco

Gastrodia anti-fungal protein from the orchid Gastrodia elata confers disease resistance to root pathogens in transgenic tobacco

Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Biological diversity of Rhizoctonia solani (AG‐3) in a northern potato‐cultivation environment in Finland

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