Evaluation of Gamavit effectiveness for correction of renal anemia in cats with chronic kidney disease: a controlled randomized study

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A pharmacokinetic study of the absorption into the bloodstream, bioavailability and excretion of Gamavit from the body after intramuscular administration to laboratory mini-pigs was conducted. Quantitative determination was carried out by HPLC using a fluorimetric detector, for which Gamavit was labeled with Cy5 dye, which was then used for mini-pigs inoculation. The developed methods for determining Gamavit in the blood and feces were validated according to the following validation parameters: selectivity, calibration curve, accuracy, precision, limit of quantitative determination, sample transfer, and sample stability. The confirmed analytical range of the method for Gamavit detection in blood plasma and feces was 1.00…50.0 mcg/ml. Maximum concentration of Gamavit in the blood of mini-pigs after a single intramuscular injection was 30.97 mcg/ml and was reached on average 15 minutes after administration. 24 hours following administration, Gamavit was still detected in the blood in insignificant amounts. The average half-life of Gamavit in the blood is 8.64±3.50 hours. After administration at a dose of 0.1 ml/kg, the clearance of the drug is 1.27 l/kg * h, the excretion rate at an effective concentration of 30 mg/l is 38 mg/kg*h, and the maintenance dose when using the drug 1 time a day is 0.9…1.0 ml. The detection of the label in the feces of the studied animals indicates that one of the ways Gamavit removal is excretion with the help of bile acids, as well as partial excretion with feces.

Section: Introductionunclassified

Study of the parameters of absorption into the bloodstream and excretion of the drug Gamavit after a single administration to laboratory mini-pigs

Narovlyanskiy

Санин

Смирнов

et al. 2021

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Phosprenyl usage as part of the complex therapy of feline chronic coronavirus infection complicated by toxoplasmosis

Savoyskaya¹,

Санин

Ogorodnikova³

et al. 2022

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Feline enteric coronavirus (FECV) infects enterocytes and causes inflammation of the intestinal mucosa in young cats, resulting in diarrhea. In adult cats the infection is usually asymptomatic, but the constant circulation of FECV in the feline population increases the likelihood of mutation, as a result of which about 5-10% of infected cats develop a virulent strain of the virus that causes usually lethal feline infectious peritonitis – FIP. The purpose of this study was to study the effectiveness of Phosprenyl (PP), an immunomodulator with antiviral activity (active substance - polyprenyl phosphate), in the treatment of chronic coronavirus infection of cats, partially complicated by toxoplasmosis. A total of 117 cats were under observation, 10 of which also had toxoplasmosis. The diagnosis was confirmed by the determination of antibodies by the methods of IHA and ELISA, as well as by ultrasound examination of the abdominal organs. Prescribed treatment: Phosprenyl per os, 1 ml , twice a day, course 2-4 months; Gamavit daily, s/c, course 10 days, with a repeated course after 3 weeks; 3-4 times. As symptomatic therapy, drugs were used primarily to improve the condition of the stool, with toxoplasmosis - Fansidar. At the end of treatment symptoms of gastrointestinal tract damage disappeared in all the patients. If before the start of therapy, the majority of patients demonstrated changes characteristic of anemia (a significant decrease in the number of red blood cells and hemoglobin levels), then after 2-4 months, the indices of erythropoiesis completely normalized. In patients with coronavirus infection complicated by toxoplasmosis, anemia was detected by CBC test, as well as an increase in the number of neutrophils. In the biochemical profile of these patients: increased activity of the enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was found as well as hyperbilirubinemia, which indicates greater liver damage and a decrease in its detoxification function during the course of the disease caused simultaneously by coronavirus and toxoplasmas. At the end of therapy, these indices also returned to normal. Thus, the use of Phosprenyl for 2-4 months reduces the duration of the disease of coronavirus infection in cats (including cases complicated by toxoplasmosis), prevents its exacerbation and possible fatal outcome. Long-term use of PP in cats with a chronic form of coronavirus infection is recommended.

Supplementation of feline hemoplasmosis treatment protocol with Gamavit and Phosprenyl increases the effectiveness of anemia correction: a retrospective analysis

Savoyskaya

2024

This study includes clinical data collected from 2016 to 2023. In total, hemoplasmosis was detected in 102 cats (63 females and 39 males) aged from 5 months to 8 years. Of these, 34 cats were diagnosed with a severe form of the disease, and 68 with a mild one. In cats with severe hemoplasmosis, in 45 % of cases, the disease was complicated by chronic renal failure. Also, 5 % of them were chronic carriers of toxoplasmas. Most of the animals were taken from shelters, or were homeless. One of the leading clinical signs of hemoplasmosis is anemia, which develops as a result of mass hemolysis of infected red blood cells. The aim of this work was to eliminate anemia and accelerate the normalization of hematopoiesis in cats with hemoplasmosis. For the treatment of 45 animals (control group), a standard therapy regimen was used, which included antibiotics and glucocorticoids, whereas for therapy regimen of remaining 57 cats (experimental group) Gamavit and Phosprenyl were added. Using this therapeutic scheme, early relief of anemia, stimulation of erythropoiesis, restoration of blood formula and normalization of liver function were noted as well as acceleration of clinical recovery of sick animals which occurred faster by 4…5 days.