Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes

Baránek, Miroslav; Kadlec, Malvina; Raddová, Jana; Vachůn, Miroslav; Pidra, M.

doi:10.17221/6114-cjgpb

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…Forty-six polymorphic bands (43%) were identified with 21 RAPD primers in this study. Similar results were shown by BARANEK et al, (2002), who detected 122 highly reproducible fragments with 22 RAPD primers, and 55 of them were polymorphic (46%). As compared to the results of other authors, polymorphism found in our study had reached slightly higher values.…”

Section: Rapd Datasupporting

confidence: 83%

Genetic relatedness of soybean genotypes based on agromorphological traits and RAPD markers

et al. 2014

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Modern agriculture, breeding procedures, as well as competition among breeding institutions contribute to further reduction of already narrowed diversity of soybean commercial varieties. The objective of the study was to characterize eighteen soybean cultivars from three different breeding programs for agro-morphological traits and to reveal genetic diversity using molecular markers. Morphological description was performed with 13 qualitative and 9 quantitative traits. The genetic relationships were estimated using 21 RAPD markers. PIC was calculated for RAPD data, while the diversity of qualitative traits was described by Shannon genetic diversity index. Cluster analysis based on qualitative morphological characters showed clear separation of genotypes on the basis of their plant growth type. PC analysis performed for quantitative traits divided genotypes according to their maturity group. Grouping pattern based on molecular marker data was in agreement with pedigree of cultivars. A great similarity was found, primarily between the varieties under the same institution, and then among all examined varieties. Comparison of three methods in the assessment of diversity indicated that morphological markers might provide useful information in breeding process and allow classification by pedigree to some extent, but RAPD markers were found to be superior in assessing differences among genetically very similar genotypes. [Projekat Ministarstva nauke Republike Srbije, br. TR-31068]

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Section: Rapd Datasupporting

confidence: 83%

Genetic relatedness of soybean genotypes based on agromorphological traits and RAPD markers

et al. 2014

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“…The use of RAPDs for analyzing soybean genome was started in the early 1990s. The RAPD markers have been widely used for genetic diversity study of soybean germplasm (Correa et al, 1999;Baranek et al, 2002;Nikolic et al, 2007;Peric et al, 2008a) (Fig. 3.).…”

Section: Randomly Amplified Polymorphic Dnamentioning

confidence: 99%

Genetic Improvement: Molecular-Based Strategies

Sudarić¹,

Vratarić²,

Drinić³

2011

Soybean - Molecular Aspects of Breeding

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“…Molecular markers are known as one of the best approaches to study genetic material as well as to assess genetic variation in crop gene pools (Badr, 2008;Mondini et al, 2009;Wang et al, 2016). A number of PCR based molecular markers such as Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Amplified Fragment Length Polymporhism (AFLP) have been used to assess genetic variability and cultivar identification in soybean (Barakat, 2004;Baránek et al, 2002;Chowdhury et al, 2001;El-Kholy, 2013) and other plant populations (Hoque and Hasan, 2012;Lin et al, 2009). RAPD is often used successfully to assess genetic diversity among species as it is fast, less technical and expensive and may reveal dominant molecular marker of good potential (Badr et al, 2012;Prasanthi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Biodiversity assessment of African locust bean (Parkia biglobosa) accessions from Savanna and Forest zones of Nigeria as revealed by seed storage proteins and RAPD markers

Akin-Idowu¹,

Aduloju²,

Akinyoola³

et al. 2021

GenResJ

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Understanding the level and distribution of genetic diversity in African locust bean (Parkia biglobosa) would strengthen breeding and conservation programmes towards domestication and sustainable use of this species. Sixteen accessions of P. globosa were assessed for variability based on seed morphology, seed protein and DNA profiling. Significant variation in seed characteristics were observed across locations. Seed protein profiling by SDS-PAGE revealed homogeneity as most bands were found common in all accessions, indicating that the protein profiles are highly conserved. Protein profiling separated the 16 accessions into four major clusters at 0.93 similarity coefficient. Most accessions grouping into Cluster 1 had a similarity coefficient of close to 100% and were from the Derived Savanna suggesting the presence of duplicates. Accessions NH/2016/P14, NH/2016/P03 and NH/2016/P04 grouped into clusters II, III and IV; respectively. Sixteen RAPD markers generated a total of 256 bands of which 63.67% were polymorphic. Gene diversity ranged from 0.41 to 0.93 and Polymorphic Information Content (PIC) from 0.39 to 0.93. The RAPD-based dendrogram separated accessions into six groups at 0.68 similarity coefficient. Based on a polymorphic seed storage protein marker a genetically distinct accession NH/2016/P04 could be exploited for breeding purposes. The homogeneity of alleles and narrow genetic base as revealed by RAPD and SDS-PAGE analyses suggests possible loss of intraspecific genetic diversity. Thus, intensification of germplasm collections across the different agroecological zones and characterization using specific markers will give a better understanding of diversity of P. biglobosa in order to enhance selection towards conservation, breeding and sustainable utilization.

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Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes

Cited by 10 publications

References 16 publications

Genetic relatedness of soybean genotypes based on agromorphological traits and RAPD markers

Genetic relatedness of soybean genotypes based on agromorphological traits and RAPD markers

Genetic Improvement: Molecular-Based Strategies

Biodiversity assessment of African locust bean (Parkia biglobosa) accessions from Savanna and Forest zones of Nigeria as revealed by seed storage proteins and RAPD markers

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