Evaluation of Genetic Diversity in Faba bean (Vicia faba L.) Genotypes using Seed Protein and Isozymes Electrophoresis

Pradhan, A. M.; Shahi, V. K.; Sinha, S. K.

doi:10.1007/s40009-014-0244-5

Cited by 4 publications

(5 citation statements)

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“…Cultivar identification has attained critical importance in the national and international seed programme as it is realized that if true to type seed of a variety is not available then the entire crop production programme may fail. In this direction earlier we reported the characterization of these germplasms on the basis of different storage proteins and isozymes (Pradhan et al 2014). Thus it is of paramount importance to characterize varieties for their correct identification.…”

Section: Resultsmentioning

confidence: 94%

“…The investigations were carried out on different germplasms of faba bean which were collected from farmer's field of different agro-climatic regions of Bihar, India (Pradhan et al 2014). All the germplasms were grown in University Research Farm (Rajendra Agricultural University, RAU, Bihar, India) under normal agronomic conditions and the dried seeds were used in present study.…”

Section: Methodsmentioning

confidence: 99%

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Nutritional and antinutritional attributes of faba bean (Vicia faba L.) germplasms growing in Bihar, India

Kumar

Agarwal

Prasad

et al. 2014

Physiol Mol Biol Plants

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Eleven germplasms of faba bean seeds from four agroclimatic regions of Bihar, India, have been investigated to estimate their nutritional (soluble protein, free amino acids, starch, reducing and non reducing sugar, total soluble sugar) and antinutritional (total extractable phenol and condensed tannin/proanthocyanidin) parameters. These parameters were found in varying concentration in all genotypes studied. The highest concentration of total extractable phenol and proanthocyanidin (condensed tannin) (2.56 and 1.59 % leucocyanidin equivalents respectively on dry matter basis) were found in Samastipur while the lowest from Patna (0.95 and 0.426 % leucocyanidin equivalent on dry matter basis). The different nutritional parameters were also found to be in variable concentration among different germplasms viz. total soluble protein≈20-32 %, free amino acids≈188-348 mg/ 100 g, starch≈27-33 %, reducing sugars≈85-188 mg/100 g, non reducing sugars≈0.7-1.7 % and total soluble sugars≈ 0.8-1.9 %.

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Section: Resultsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Nutritional and antinutritional attributes of faba bean (Vicia faba L.) germplasms growing in Bihar, India

Kumar

Agarwal

Prasad

et al. 2014

Physiol Mol Biol Plants

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“…Characterization of the genetic variation in the available germplasms is important for further crop improvement which facilitates the transfer of useful genes among cultivated species and to improve yield parameters to impart resistance to biotic and abiotic stresses. 6 Our work demonstrated that different faba bean cultivars have different level of extractable phenol and proanthocynidin 7 in seed extract ( Figure 1A). The whole tissue or histo-chemical localization of CTs may serve the purpose to determine its content in different germplasms for selection of better parent for conventional and molecular breeding to address this nutritional problem of this pulse crop.…”

Section: Way Forward For Improvementmentioning

confidence: 67%

Condensed tannin: a major anti-nutritional constituent of faba bean (Vicia faba L.)

Sinha

Kumar

2018

HIJ

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Nutritionally faba bean (Vicia faba L.) is one of the important legume crops cultivated for both food and feed purposes. However, in some parts of the world it has not gained status of commercial legume crop and continues to be grown in marginal agricultural fields with almost no agricultural inputs. Despite of having nutritional as well as medicinal properties, it is not being cultivated in large scale because of one or other anti-nutritional constituents present in its edible parts. Condensed tannins are one of the major anti-nutritional factors found in edible parts of this crop which confers noticeable astringency and inhibition in digestion process. Molecular breeding and genetic engineering approaches may provide successful way to improve this crop to reduce the content of condensed tannin in the seed coat. Germplasm characterization for identification of best parents and identification of crucial genes/QTLs are some of the initial steps for improvement of this crop. Genomic information available in model and other legume crops will also serve as important resources for taking suitable approach.

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“…Despite this, there were many other evolutionary studies to clarify the relationships among Vicia species by determining the electrophoretic seed proteins patterns [51,[61][62][63][64][65][66][67][68]. It was noticeable that most of previous systematic classification and genetic relationship studies on genus Vicia using SDS-PAGE have been concentrated on faba bean [69][70][71][72][73], which indicates that SDS-PAGE was a useful tool for genetic diversity analysis of faba bean.…”

Section: Discussionmentioning

confidence: 99%

Genetic relationship study of some Vicia species by FISH and total seed storage protein patterns

Ali

Osman

2020

Journal of Genetic Engineering and Biotechnology

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Background: Genus Vicia is a member of family Fabaceae and comprises 180 to 210 species. The most important species is faba bean (Vicia faba) which is still one of the most favourable grain legumes over all the world. The genus contains some additional food crops and a number of forage plants and some other weedy strains such as Vicia angustifolia and Vicia cordata. The aim of the present investigation is to elucidate the phylogenetic relationships among four Vicia species, two species (Vicia angustifolia L. ssp. Angustifolia (2n = 12) and Vicia cordata wulfen ex Hoppe (2n = 10)) belong to section Vicia, Vicia dalmatica A. Kern (2n = 12, section Cracca), and Vicia johannis tamamsch (2n = 14, section Faba). Results: Two tools have been applied to identify the genetic relationships among the examined species, double fluorescence in situ hybridization (FISH) has been used to localize the sites of 5S and 45S rDNA, and sodium dodecyl sulfate-poly acrylamide gel electrophoretic (SDS-PAGE) patterns of total seed storage protein fractions. Double FISH experiment has not shown any variation in the loci number, but the positions along the chromosomes were different; both Vicia johannis and Vicia dalmatica exhibited the same interstitial 45S rRNA gene loci, while Vicia angustifolia and Vicia cordata have shown single large stretched 45S rRNA loci almost at the terminal region of the shortest chromosome. It could be concluded from the similarity matrix among the Vicia species as computed according to Jaccard coefficient from the SDS-PAGE, that V. cordata is similar to V. angustifolia and V. dalmatica by a percentage of 73 and 69%, respectively, and the most related species to V. johannis is V. dalmatica (~64%). Conclusion: FISH and SDS-PAGE of the total seed storage proteins together reflected the similar genetic relationship among the studied species as fellows, V. angustifolia is more related to V. cordata then comes V. dalmatica and then V. johannis which is at a distal position from the other species.

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Evaluation of Genetic Diversity in Faba bean (Vicia faba L.) Genotypes using Seed Protein and Isozymes Electrophoresis

Cited by 4 publications

References 11 publications

Nutritional and antinutritional attributes of faba bean (Vicia faba L.) germplasms growing in Bihar, India

Nutritional and antinutritional attributes of faba bean (Vicia faba L.) germplasms growing in Bihar, India

Condensed tannin: a major anti-nutritional constituent of faba bean (Vicia faba L.)

Genetic relationship study of some Vicia species by FISH and total seed storage protein patterns

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