Evaluation of genetic diversity within asparagus germplasm based on morphological traits and ISSR markers

Chen, Helong; Guo, Anping; Jian, Wang; Gao, Jianming; Zhang, Shiqing; Zheng, Jianhua; Xing, Huang; Xi, Jingen; Yi, Kexian

doi:10.1007/s12298-019-00738-5

Cited by 24 publications

(13 citation statements)

References 44 publications

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“…Greater Fst value than 0.15 is an indicator of significant genetic differentiation (Frankham et al, 2010). In the present study, mean Fst value was computed as 0.4212 which was higher than previous studies (Chen et al, 2020;Ciancaleoni et al, 2018;Yousef et al, 2018) or similar to some of them (Sun et al, 2018;Tian et al, 2017). Likewise, the high value of expected heterozygosity is desirable for high genetic variation in the population.…”

Section: Discussioncontrasting

confidence: 64%

“…ISSR markers have been used for genetic diversity analysis and molecular characterization of different plants like bread wheat (El-Sherbeny et al, 2020), taramira (Zafar-Pashanezhad et al, 2019), ginger (Baruah et al, 2019), kewda (Nasim et al, 2020), cassava (Afonso et al, 2019), flax (Ahmed et al, 2019), asparagus (Chen et al, 2020), and anise (Akçali-Giachino, 2020). Besides, ISSR markers were used for genetic diversity analysis and molecular characterization of Brassica by different researchers (Kalia et al, 2017;Kaur et al, 2014;Koch et al, 2017;Kong et al, 2011;Mohammadin et al, 2017;Takahashi et al, 2019;Verma et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis of some species in Brassicaceae family with ISSR markers

Maraş-Vanlioğlu¹,

Yaman²,

Kayaçetin³

2020

biost

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Brassicaceae is one of the biggest family which have thousands of species all around the world. In order to use wild mustard in a breeding process, their genetic kinship levels must be defined. Inter simple sequence repeats (ISSRs) are one of the common markers to evaluate genetic diversity. Here, 28 mustard genotypes representing four taxa, 17 of Brassica juncea, 2 of B. nigra, 2 of B. rapa, and 7 of B. arvensis, were investigated with seven ISSR primers. Totally, 160 bands were scored out of which 88.75% showed polymorphism. The polymorphism information content (PIC) varied from 0.25 to 0.40. The average heterozygosity (), multiplex ratio (), marker index (), and resolving power () were calculated as 0.33, 9.07, 2.99, and 8.29, respectively. STRUCTURE (v. 2.3.4) analysis unraveled two subpopulations (K=2). The dendrogram, constructed based on Jaccard similarity coefficient using the Unweighted Pair Group Average (UPGMA), in which, the first branch consisted of B. juncea, B. nigra and B. rapa, and the second branch consisted of B. arvensis, supported the results of STRUCTURE analysis. Additionally, principal component analysis (PCA) analysis supported the dendrogram and clearly separated the four taxa. This study showed that ISSRs would be useful to determine the genetic diversity in the Brassicaceae family.

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Section: Discussioncontrasting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of some species in Brassicaceae family with ISSR markers

Maraş-Vanlioğlu¹,

Yaman²,

Kayaçetin³

2020

biost

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“…Only in few regions like southern states and Uttarakhand the wild stocks of A. racemosus are utilized as vegetable [ 44 ]. In both the food industry and pharmaceutical industry, the demand of Asparagus produce is high due to presence of different secondary metabolites which includes rutin, ascorbic acid, tocopherol, ferulic acid, and glutathione [ 45 ]. However, the natural stocks of these species cannot fulfill this demand.…”

Section: Discussionmentioning

confidence: 99%

Analysis of genetic diversity and population structure in Asparagus species using SSR markers

Kapoor

Mawal

Sharma³

et al. 2020

Journal of Genetic Engineering and Biotechnology

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Background Various Asparagus species constitute the significant vegetable and medicinal genetic resource throughout the world. Asparagus species serve as important commodity of food and pharmaceutical industries in India. A diverse collection of Asparagus species from different localities of Northwest India was investigated for its genetic diversity using simple sequence repeat (SSR) markers. Results Polymorphic SSR markers revealed high genetic diversity. Primer SSR-15 amplified maximum of 8 fragments while 3 primers, namely, SSR-43, SSR-63, and AGA1 amplified minimum of 3 fragments. Collectively, 122 alleles were amplified in a range between 3 and 8 with an average of 5 alleles per marker. The size of the amplified alleles ranged between 90 and 680 base pairs. Polymorphism information content (PIC) value varied from a highest value of 0.499 in primer AGA1 to a lowest value of 0.231 in primer SSR-63 with a mean value of 0.376 showing considerable SSR polymorphism. Dendrogram developed on the basis of Jaccard’s similarity coefficient and neighbor-joining tree segregated all the studied Asparagus species into two discrete groups. Structure analysis based on Bayesian clustering allocated different accessions to two independent clusters and exhibited low level of individual admixture. Conclusions The genetic diversity analysis showed a conservative genetic background for maximum species of asparagus. Only Accessions of Asparagus adscendens were split into two diverse clusters suggesting a wide genetic base of this species as compared to other species. Overall genetic diversity was high, and this germplasm of Asparagus can be used in future improvement programs. The findings of current research on Asparagus germplasm can make a momentous contribution to initiatives of interbreeding, conservation, and improvement of Asparagus in future.

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“…The same species or cultivars may have signi cant variation in some traits and have the potential to produce a complex phenomenon of intra-species polymorphism. Morphological variations are important because they generally show high phenotypic plasticity (Chen et al 2020). If these asparagus are included in possible breeding studies, it is important to de ne the superior locations in terms of some characteristics (such as spear size, weight).…”

Section: Mousavizadeh Et Al (2015)mentioning

confidence: 99%

Determination of natural distribution areas and some agro-morphological characteristics with sexual dimorphism of wild asparagus (Asparagus officinalis L.) in Iğdır plain-Turkey

Özden

Şeyran²,

Koçak³

et al. 2022

Genet Resour Crop Evol

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Globally, interest in wild plants has been on the rise in recent years. The potential of wild plants to be used in breeding for sustainable agriculture and people's preference for consumption attract research attentions. The study was aimed to investigate the natural distribution of Asparagus o cinalis L. taxon, along the Iğdır plain, one of the largest micro-climate regions of Turkey and consumed by the people of the region. The research was also motivated to evaluate wild Asparagus species using some agro-morphological characteristics with sexual dimorphism, for a two years study period. It has been identi ed that asparagus spreads along the Aras river forming the border of Armenia at altitudes of 800-1076 masl in the region and most densely populated in Karakoyunlu district.The result of the present investigation revealed a wide range of variations for agro-morphological characteristics like days to fruit ripening (76-91 d), edible spear length (168.21-315.34 mm), diameter (6.30-10.86 mm), fresh weight (5.27-19.52 g), dry weight (0.38-1.55 g), number of spear (8-26 plant -1 ), number of fruit (61-2467 spear -1 ), fruit weight (8.0-258.3 g spear -1 ), male plant length (1116.9-2.163.7 mm), female plant length (1219.4-2110.2 mm), male pedicle length (3.72-4.17 mm) and female pedicle length (3.21-3.54 mm) were observed, which can be useful for breeding purposes. Cluster constellation analysis was conducted to investigate the genetic diversity of 31 asparagus genotypes. According to the agro-morphological properties, asparagus were divided into 2 main groups and 4 sub-groups (A; A1, A2 and B; B1, B2). Aralık district samples were mostly collected in the B1 (pink), Karakoyunlu district in the B2 (blue), Tuzluca district in the Al (red) and A2 (green), and Iğdır district mostly in the B2 (blue) cluster. As a result, Karakoyunlu were found superior with the highest asparagus density and edible spear yield as compared to other districts. In order protect the habitat of wild asparagus from excessive consumption and losses due to climate changes, seeds should be collected from these areas to be used for cultivation and breeding purposes. In addition, it is advisable to keep seeds in gene banks for sustainable agriculture and protection of genetic diversity.

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Evaluation of genetic diversity within asparagus germplasm based on morphological traits and ISSR markers

Cited by 24 publications

References 44 publications

Genetic diversity analysis of some species in Brassicaceae family with ISSR markers

Genetic diversity analysis of some species in Brassicaceae family with ISSR markers

Analysis of genetic diversity and population structure in Asparagus species using SSR markers

Determination of natural distribution areas and some agro-morphological characteristics with sexual dimorphism of wild asparagus (Asparagus officinalis L.) in Iğdır plain-Turkey

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