Evaluation of Genetic Homogeneity in Tissue Culture Regenerates of Jatropha curcas L. using Flow Cytometer and DNA-based Molecular Markers

Rathore, Mangal S.; Yadav, Pawan K.; Mastan, Shaik G.; Prakash, Ch. Ravi; Singh, Aneesha; Agarwal, Pradeep K.

doi:10.1007/s12010-013-0517-3

Cited by 23 publications

(23 citation statements)

References 29 publications

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“…Morphologically saplings were similar andverifi ed by molecular marker (Rathore et al 2014 ), and suggesting plants -were true to type. The survival rate of 60-70 % was achieved in fi eld (Rajore and Batra 2005 ).…”

Section: Rooting Hardening and Field Trialsmentioning

confidence: 80%

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Micropropagation of Plants

Singh

2015

Plant Biology and Biotechnology

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Micropropagation is a rapid multiplication of a selected plant using in vitro culture techniques. In this chapter various aspects of micropropagation have been discussed. The propagation of selected plant through micropropagation would be useful for raising plantation using apical and nodal segment. They are best for micropropagation and mostly result in true to type plants. These segments upon the subsequent subcultures result in a number of multiple shoots. These multiple shoots on elongation allowed to root in vitro. After rooting, they are in vitro hardened and transferred to fi eld. The potential of plant tissue culture is well recognized, as it increases agricultural production and generates rural employment. But the high cost of production on micropropagation is a major bottleneck. Low-cost protocol development can popularize this method. High multiplication rate, use of low-cost chemicals, high rooting and survival percentage, and use of ex vitro rooting method can minimize the expense of any protocol. Presently, both the developing and the developed countries require lowcost technologies to progressively reduce the cost of production. The performance of tissue plants generally better than cutting and seedling. Initially, some morphological changes occur in micropropagated plant, but in the course of time, they minimize. Somaclonal variation caused during callus cultures can be used to generate variants for hybridization. Variation in phenotype of plants produced in tissue culture can be characterized as either temporary or permanent. Temporary variation includes increased branching, greater susceptibility to disease, and lack of uniform response. Micropropagated plants need to be monitored to get long-term data for its

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Section: Rooting Hardening and Field Trialsmentioning

confidence: 80%

“…CSIR-CSMCRI in collaboration with MNRE (Ministry of New and Renewable Energy) worked on project large-scale micropropagation of Jatropha curcas and generated cultures on a large scale and developed plants were well established in the fi eld. Micropropagated plants were found genetically stable (Rathore et al 2014 ).…”

Section: Rooting Hardening and Field Trialsmentioning

confidence: 99%

Micropropagation of Plants

Singh

2015

Plant Biology and Biotechnology

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“…Occurrence of TC-induced variations at any stage of development of plantlets is one of the major problems associated with micropropagation among donor plant and its clones [12,13], and genetic variability should be checked after consecutive subcultures to prevent undesirable and unexpected results, which could be expressed later in growth [14]. The level of TCinduced variations depends on the several factors such as genotype, source of material (meristems, leaves, roots, etc.)…”

Section: Introductionmentioning

confidence: 99%

Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers

Akdemir

Süzerer

Tilkat

et al. 2016

Appl Biochem Biotechnol

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Determination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0.226), ISSR (0.220), and AFLP (0.241) showed that micropropagation of pistachio for different periods of time resulted in "reasonable polymorphism" among donor plant and its 18 clones. Mantel's test showed a consistence polymorphism level between marker systems based on similarity matrices. In conclusion, this is the first study on occurrence of genetic variability in long-term micropropagated mature pistachio plantlets. The obtained results clearly indicated that different marker approaches used in this study are reliable for assessing tissue culture-induced variations in long-term cultured pistachio plantlets.

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“…Thus, it is important to determine any variation that occurs at an early stage of in vitro development [22]. Some molecular methods have been previously applied for genetic stability studies in J. curcas, such as the use of amplified fragment length polymorphisms (AFLPs) [23], random amplified polymorphic DNA (RAPD) [24] and flow cytometry [25]. The use of inter simple sequence repeats (ISSRs) offers unique advantages over other molecular markers, since their application does not require any genomic information of the target species, it only requires a small amount of template DNA, is rapidly performed [26] and is highly efficient in detecting highly polymorphic DNA among Jatropha genotypes [27,28].…”

Section: Introductionmentioning

confidence: 99%

“…The use of inter simple sequence repeats (ISSRs) offers unique advantages over other molecular markers, since their application does not require any genomic information of the target species, it only requires a small amount of template DNA, is rapidly performed [26] and is highly efficient in detecting highly polymorphic DNA among Jatropha genotypes [27,28]. An ISSR marker system has been successfully used to detect somaclonal variation in J. curcas [25,29,30].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Nasir

Anuar

Zainuddin

et al. 2016

Biotechnology & Biotechnological Equipment

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Genetic homogeneity is known to be the most important prerequisite in the micropropagation of Jatropha curcas L. to produce true-to-type plants. The detection of genetic homogeneity in clonal micropropagation for elite plants at an early stage is required, to avoid any increase in variation in the next stage of micropropagation. The genetic homogeneity was assessed during shoot bud formation from petiole explants of J.curcas (P1 £ P3) hybrid with different concentrations of thidiazuron (TDZ) in a range of 0.5-4.0 mg/L using inter simple sequence repeat (ISSR) markers. Out of 23 ISSR primers, 16 primers produced clear, distinct and reproducible bands. A total of 96 bands, ranging in size from 100 to 1013 bp were generated. Based on the band data, a total of 94 bands were monomorphic (98%) and two bands were polymorphic (2%). All banding patterns from the shoot buds induced by 0.5, 1.0 and 2.0 mg/L TDZ were monomorphic, but 4.0 mg/L gave 2% polymorphism. These findings indicated that concentrations of 0.5, 1.0 and 2.0 mg/L TDZ did not trigger any somaclonal variation and could, therefore, be considered suitable for application in clonal micropropagation of J. curcas hybrid using petioles as explant material.

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Evaluation of Genetic Homogeneity in Tissue Culture Regenerates of Jatropha curcas L. using Flow Cytometer and DNA-based Molecular Markers

Cited by 23 publications

References 29 publications

Micropropagation of Plants

Micropropagation of Plants

Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers

Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

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