Evaluation of genetic stability in cryopreserved Prunus

Helliot, B.; Madur, D.; Dirlewanger, Elisabeth; Boucaud, Marie‐Thérèse de

doi:10.1079/ivp2002325

Cited by 32 publications

(26 citation statements)

References 20 publications

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“…Alternatively, according to Regina et al (2002), in birch DNA competition among RAPD loci in the random primed PCR may be a major reason for varying amplification results, and this phenomenon most probably caused variation in the intensity of some fragments seen in the present work. Interpretation of the cytological examination, on the other hand, can run into technical difficulties if the chromosomes of the studied species are small and numerous, or have a low staining ability against cytoplasm of high density, as reported for example in Prunus (Helliot et al, 2002) and also experienced in the present silver birch work.…”

Section: Discussionmentioning

confidence: 84%

“…Similarly, molecular marker studies of woody species e.g. hybrid aspen (Jokipii et al, 2004), Prunus (Helliot et al, 2002), grape (Vitis sp. ), and kiwi (Actinidia sp.)…”

Section: Discussionmentioning

confidence: 99%

“…Using cytological examination, no variation in ploidy level or chromosome number was detected in chromosomal preparations in either herbaceous Hypericum (Urbanova´et al, 2002) or in woody Prunus sp. (Helliot et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

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Genome fidelity during short- and long-term tissue culture and differentially cryostored meristems of silver birch (Betula pendula)

Ryynänen¹,

Aronen²

2005

Plant Cell Tiss Organ Cult

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Clonal trueness of micropropagated or cryopreserved material is essential, especially with long-living tree species. In this study, the growth rate and morphology of regenerated silver birch (Betula pendula Roth) plants growing in the nursery were evaluated after different treatments: short-term (14 months) and longterm (70 months) tissue culture periods, cryostorage of in vivo buds and cryopreservation of in vitro shoot apices using four different slow cooling cryopreservation protocols with PGD (10% PEG, 10% glucose, 10% DMSO) as cryoprotectant. Genetic fidelity of the regenerated plants compared to the original donor trees was evaluated using RAPD assays together with chromosome analysis. The regenerated plants showed no genetic or phenotypic changes, and can thus be considered as reliable material for any research, breeding or silvicultural activities.

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Section: Discussionmentioning

confidence: 84%

“…Similarly, molecular marker studies of woody species e.g. hybrid aspen (Jokipii et al, 2004), Prunus (Helliot et al, 2002), grape (Vitis sp. ), and kiwi (Actinidia sp.)…”

Section: Discussionmentioning

confidence: 99%

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Genome fidelity during short- and long-term tissue culture and differentially cryostored meristems of silver birch (Betula pendula)

Ryynänen¹,

Aronen²

2005

Plant Cell Tiss Organ Cult

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“…Various studies with molecular markers have been performed to asses somaclonal variation in cryopreserved plant material (Harding 2004, Harding et al 2005, Helliot et al 2002. Among the DNA analysis techniques used, randomly amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) analysis have also been applied to evaluate genetic stability of the material surviving cryopreservation and in most of the cases have not provided evidence for genetic variation.…”

Section: Introductionmentioning

confidence: 99%

Genetic stability of wild pear (Pyrus pyraster, Burgsd) after cryopreservation by encapsulation dehydration

Condello¹,

Palombi²,

Tonelli³

et al. 2008

AFSci

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Shoot tips of Pyrus pyraster were successfully cryopreserved by encapsulation-dehydration. Na--alginate beads each containing one shoot tip, dehydrated for 2 days in 0.75M sucrose and desiccated to 20% moisture content (fresh weight basis), gave 60% recovery after exposure to liquid nitrogen. Regenerated shoots showed no differences in length and leaf shape compared to the mother plant. Multiplication rate and rooting ability of cryopreserved shoots were lower than those of untreated controls after one subculture, but were completely restored following the third subculture. Fifteen cryopreserved lines derived from single buds were used for genetic analyses by RAPDs and SSRs, in comparison with the mother plant. In RAPD analysis, of a total of 24 primers, only 15 showed reproducible and well resolved bands and were further used. These primers produced a total of 66 fragments ranging from about 500 to 2500 base pair size. SSR (microsatellite) marker amplification was performed using 19 primers which produced 57 reproducible fragments. Microsatellites fragments ranged from 60 to 600 base pairs. Both RAPDs and SSRs did not reveal any polymorphism between cryopreserved lines and the original genotype, suggesting that cryopreservation, using encapsulation-dehydration, does not affect genetic stability of wild pear.

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“…Most studies have confirmed the presence of genetic stability (Castillo et al, 2010;Helliot et al, 2002) and where changes in the genome have been found, such as in sugarcane and potato with RFLP markers, the changes could not be related to the process of cryopreservation itself (Castillo et al, 2010;Harding, 2004 (Channuntapipat et al, 2003), papaya (Kaity et al, 2008), chrysanthemum (Martín & González-Benito, 2006), Ribes , strawberry (Hao et al, 2002a), citrus (Hao et al, 2002b) and potato (Kaczmarczyk et al, 2010). Changes in methylation might be caused by stressful in vitro conditions, osmotic dehydration and the application of cryoprotective agents (Harding, 2004).…”

Section: Genetic and Epigenetic Stabilitymentioning

confidence: 99%