Evaluation of genetic toxicity caused by acid mine drainage of coal mines on fish fauna of Simsang River, Garohills, Meghalaya, India

Talukdar, B.; Kalita, H. K.; Baishya, R.; Basumatary, S.; Sarma, Dandadhar

doi:10.1016/j.ecoenv.2016.05.011

Cited by 23 publications

(9 citation statements)

References 31 publications

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“…Several ecotoxicological characteristics of C. punctatus, such as easy acclimatization to laboratory conditions, availability throughout the seasons, and commercial importance, make this species an excellent test specimen for toxicity studies (Nwani et al 2010;Talukdar et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Sharma

Chadha

2017

Environ Sci Pollut Res

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The present study investigated the intertissue differences in genotoxicity induction in the fish, Channa punctatus, in response to 4-nonylphenol. The lethal concentration of 50 (LC) was estimated using a semi-static system. Fish were subjected to three sublethal concentrations of 4-nonylphenol (NP) for 24, 48, 72, and 96 h. Liver, gill, and kidney tissues were analyzed, and the genotoxicity was estimated using the micronucleus test and the comet assay. The frequency of micronucleated cells (MNCs), binucleated cells (BNCs), aberrant cells, and tail moment (TM) increased significantly. The time for maximum induction of genotoxicity for all the parameters considered in the micronucleus assay was 72 h of exposure, whereas the TM was highest at 24 h of exposure followed by a decline. The gill and liver showed higher genotoxicity than kidney tissue demonstrating organ-specific susceptibilities to NP in fish C. punctatus.

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Section: Introductionmentioning

confidence: 99%

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Sharma

Chadha

2017

Environ Sci Pollut Res

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“…%DNA in tail is a representation of single-and double-stranded DNA intensity within the tail region, giving an insight into the kinetics of gDNA unravelling in the presence of harmful substances (Hayashi et al, 2000). Due to comet class scoring data, the study observed a high %DNA in tail in cell lines exposed to U and T. Chromosomal changes might be directly related to DNA fragmentation, which has been used by many studies as a biomarker to confirm the presence of a substance causing damage-mediated DNA cleavage (Talukdar et al, 2016) Results showed that both cell lines detected genotoxins in surface water; the Vero Monkey kidney cell lines, however, showed greater sensitivity. Another study reported a dose-dependent increase in DNA fragmentation observed in primary cultures of rat and human kidney cells exposed to chemicals carcinogenic to rat kidney (Robbiano et al, 2004).…”

Section: Discussionmentioning

confidence: 96%

“…Da Silveira et al (2009 described DNA damage in Geophagus brasiliensis exposed to both un-neutralised and pH-neutralised AMD, indicating cellular genotoxicity. DNA fragmentation was reported in kidney of fishes, Channa punctata (Bloch), exposed to AMD (Talukdar et al, 2017) and genetic toxicity was established in fish fauna of Simsang River, India, caused by AMD from coal mines (Talukdar, 2016).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the genotoxic potential of water impacted by acid mine drainage from a coal mine in Mpumalanga, South Africa, using the Ames test and Comet assay

Iji

Njoya

Madikizela

et al. 2021

WSA

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Several potential genotoxins found in water samples arise from anthropogenic activities. Acid mine effluent resulting from coal mining poses serious environment concerns all over the world. The use of toxicity tests to evaluate the quality of streams add value by providing site-specific toxicological data. Treatment systems such as the use of natural wetlands (passive) or conventional physical and chemical pH-neutralised processes (active) are employed mainly to meet certain water quality guidelines. Nonetheless, potential genotoxins or residues remain which influence the quality of discharged effluents. The objective of this study was to evaluate the genotoxic potential of acid mine drainage (AMD) released into a natural stream following treatment by passive and active methods. This study aimed to identify the extent of AMD mutagenicity and genotoxicity to African Vero monkey kidney cell line and a fish gill cell line (RTgill-W1) using two assays, the Ames test, and the comet assay, as a rapid and effective screening tool. The Ames test performed without metabolic activation using Salmonella typhimurium TA98 and TA100 strains showed no indication of mutagenicity in the water samples tested. Differing results were however obtained for the comet assay using the African Vero monkey kidney cell line and a fish gill cell line (Rtgill-W1), which revealed DNA fragmentation and variations in morphologies indicative of genotoxicity in the water samples following the two treatment processes. A significant reduction in DNA damage was observed in water samples following active treatment of the AMD, evidenced by reduced damage frequency and a lowered comet score. This bioassay confirms the urgency of integrating high-throughput screening in aquatic toxicity assessment at genetic levels, giving further evidence that in-vitro bioassays can be incorporated for use in short-term genotoxicity assays. The result suggests that the comet assay proved sensitive at detecting genotoxicity, supporting the integration of this into environmental monitoring frameworks targeted at AMD-contaminated sites.

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“…To evaluate the effects of water borne contaminants, shes are most authentic experimental model because they are sensitive to surrounding environment and as being the rst recipient to toxicant, they are able to accumulate and metabolize these contaminants in their bodies similar to higher vertebrates (Ali et al 2020). Channa punctatus is available throughout the year, easy to maintain under laboratory conditions, has high commercial importance and ease of non-invasive blood collection makes this sh an excellent model for toxicological studies (Talukdar et al 2016;Kumar et al 2010;Javed et al 2017). Blood is the most important and abundant body uid that transports nutrients and oxygen to lungs and tissues of body and serves as an ideal medium for toxicological analysis.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Genotoxic And Biochemical Stress Response To Mercuric Chloride In Erythrocytes of Freshwater Fish Channa Punctatus

Gill¹,

Paul²,

Dua³

2021

Preprint

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Experiments were conducted to evaluate health of fish, Channa punctatus inhabiting mercuric chloride. Acute toxicity bioassays were performed to calculate 96h LC50 value and it was found to be 1.38mg/L. Chronic toxicity assay to investigate genotoxic effects on the erythrocytes of fish by comet assay and micronucleus assay along with alterations in blood biochemistry were evaluated. Results showed concentration and duration dependent significant DNA damage as observed by comet assay and micronucleus assay. The frequency of nuclear aberrations along with appearance of micronuclei were observed after 30 and 60 days of exposure. The blood biochemistry was studied by recording changes in levels of various biochemical parameters in blood serum and results showed the significant (p < 0.05) variations among levels of biochemical constituents such as Glucose, Lipids, Proteins, Bilirubin, Urea, Creatinine, Cholesterol, ALP, Albumin, SGOT, SGPT and Total Glycerides. The resultsindicated the stress response of specimen towards toxicant. The present study highly recommends the use of genotoxicity and blood biochemical analysis as the useful biomarker to assess toxicity in the aquatic water and hence to safeguards the surrounding ecosystem.

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Evaluation of genetic toxicity caused by acid mine drainage of coal mines on fish fauna of Simsang River, Garohills, Meghalaya, India

Cited by 23 publications

References 31 publications

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Evaluation of the genotoxic potential of water impacted by acid mine drainage from a coal mine in Mpumalanga, South Africa, using the Ames test and Comet assay

Evaluation of Genotoxic And Biochemical Stress Response To Mercuric Chloride In Erythrocytes of Freshwater Fish Channa Punctatus

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