Evaluation of Genotoxicity and Antigenotoxicity of Artepillin C in V79 Cells by the Comet and Micronucleus Assays

Abstract

Artepillin C (3,5-diprenyl-p-coumaric acid) is one of the major phenolic compounds found in Brazilian green propolis, as well as in its botanical source, Baccharis dracunculifolia DC (Asteraceae). The present study evaluated the possible genotoxic and protective activities of artepillin C, in vitro, using methyl methanesulfonate (MMS) as a positive control, by comet and micronucleus assays. The cultures of Chinese hamster lung fibroblasts (V79 cells) were treated with different concentrations of artepillin C (2.5, 5.0, 10.0, and 20 μM). In antigenotoxicity assessment, the 3 concentrations of artepillin C (2.5, 5.0, and 10.0 μM) were associated with MMS (200 μM-comet assay and 400 μM-micronucleus assay). A statistically significant increase in the DNA damage and micronucleus frequencies was observed in the culture treated with the highest concentration of the artepillin C in comparison to the control group. All concentrations of artepillin C showed protective activity in relation to MMS-induced genotoxicity, which may be due to its antioxidant properties.

Evidence of Some Natural Products with Antigenotoxic Effects. Part 2: Plants, Vegetables, and Natural Resin

“…Cultures of Chinese hamster lung fibroblasts (V79 cells) were treated with different concentrations of artepillin C (2.5 μM, 5.0 μM, 10.0 μM, and 20 μM) and combined with MMS (comet assay; 200 μM, and micronucleus assay; 400 μM). The study confirmed that all concentrations of artepillin C showed protective activity in relation to MMS-induced genotoxicity; a phenomenon that can be attributed to its antioxidant properties [201].…”

“…With these results, baccharin can be considered a chemopreventive agent, whose possible antioxidant effect was responsible for reducing the genomic and chromosomal damages. Likewise, this chemical compound was also responsible for the antigenotoxicity also demonstrated by Baccharis dracunculifolia ; the most important plant source of Brazilian green propolis [201,202,203].…”

“…Considering that baccharin is not the only phytochemical present in propolis, the same group of Oliveira (2013) [201] and the scientific group of de Azevedo Bentes Monteiro (2011) [204] explored the protective activity of artepillin C (3, 5-diprenyl p-coumaric acid-), one of the major phenolic compounds found in Brazilian green propolis. De Azevedo Bentes Monteiro Neto and colleagues analyzed the antigenotoxicity of artepillin C against the damage produced by DXR and MMS in male Swiss mice using the micronucleus and comet assays.…”

Análise da prospecção tecnológica por meio de revisão sistemática seguida de meta análise das patentes do gênero Baccharis

“…• Ação antidiabética e sensibilizadores de insulina (CHOI et al, 2011, IKEDA et al, 2011UEDA et al, 2013) • Oncológica (KIMOTO et al, 2000;KIMOTO et al, 2001a, KIMOTO et al, 2001bAKAO et al, 2003, SHIMIZU et al, 2005SHIMIZU et al, 2006;CHEN et al, 2009aCHEN et al, , 2009bMESSERLI et al, 2009;VOELKEL-JOHNSON, 2011;SZLIS-ZAKA et al, 2011b;FRANCHI et al, 2012;ISHIKAWA et al, 2012;SZLISZKA et al, 2012;DE OLIVEIRA 2013;KIM et al, 2014;MUNARI et al, 2014;FRIÓN-HERRERA et al, 2015, MORTAZAVI et al, 2015, YANG et al, 2017, YOON et al, 2017SOUZA et al, 2018) e…”

Potential chemoprotective effects of green propolis, L-lysine and celecoxib on bone marrow cells and peripheral blood lymphocytes of Wistar rats subjected to bladder chemical carcinogenesis

“…Genotoxicity could occur indirectly by hepatic metabolism of everything that is ingested or inhaled daily. In the last five years, there have been 15.000 published studies investigating antimutagenic and anticarcinogenic substances, with the majority coming from plants. The CA is used to detect genomic alterations.…”