Evaluation of genotoxicity, enzymatic alterations and cadmium accumulation in Mozambique tilapia Oreochromis mossambicus exposed to sub lethal concentrations of cadmium chloride

Naik, Akshata P.; Shyama, S.K.; D’Costa, Avelyno H.

doi:10.1016/j.enceco.2020.07.006

Cited by 12 publications

(5 citation statements)

References 37 publications

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“…Naik et al (26) also observed an increase in the frequency of MN in Mozambique tilapia (Oreochromis mossambicus) exposed to cadmium at 7.4 mg/L, 3.7 mg/L and 1.85 mg/L. Just as in the case of DNA integrity defects, MN induction decreased with time of exposure to metals, which may suggest an attempt by the organism to restore the physiological balance despite the persisting environmental pollution.…”

Section: Discussionmentioning

confidence: 91%

“…On the other hand, in the study by Singh et al (36), the frequency of MN formed as a result of exposure to cadmium was 2.75 ± 1.25%, with no micronuclei observed in the control group. Studies by Hussain et al (19) and Naik et al (26) also demonstrated that contamination with heavy metals causes an increase in the frequency of MN in erythrocytes. Hussain et al (19) reported a frequency of 5.0 ± 0.63% for single micronucleus induction and 1.44 ± 0.25% for double micronucleus induction in Rohu labeo from the Chenab River.…”

Section: Discussionmentioning

confidence: 93%

“…( 19 ) and Naik et al . ( 26 ) also demonstrated that contamination with heavy metals causes an increase in the frequency of MN in erythrocytes. Hussain et al .…”

Section: Discussionmentioning

confidence: 94%

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Genotoxic effect of cadmium and zinc in the peripheral erythrocytes of Prussian carp (Carassius gibelio B.)

Drąg‐Kozak

Kuchta-Gładysz²,

Grzesiakowska³

et al. 2022

Journal of Veterinary Research

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Introduction Cadmium and zinc are often found in aquatic environment and may accumulate in living organisms. The aim of this study was to evaluate the genotoxic effect of Cd, Zn, and their binary mixture on the peripheral blood erythrocytes of Prussian carp (Carassius gibelio B.). Material and Methods The fish were exposed to 4.0 mg/L Cd, 4.0 mg/L Zn or a mixture of 4.0 mg/L Cd and 4.0 mg/L Zn for a period of 14, 21 or 28 days. Genotoxic effects were investigated in peripheral blood cells using the comet assay and the erythrocyte micronucleus assay. Results The results demonstrated that the frequencies of micronuclei (MN) and both nuclear and cellular abnormalities in erythrocytes were significantly higher in all exposure groups as compared to the control group. The fish exposed to the mixture of Cd and Zn presented the highest frequency of MN. Furthermore, there was a decrease in the frequency of MN and an increase in the occurrence of DNA integrity defects (DNA damage) with longer time of exposure to the metals studied. Conclusion Erythrocyte micronucleus and comet assays confirmed the genotoxicity of Cd and Zn. The results of the tests applied (which showed considerable variability) suggest the involvement of various toxicity mechanisms. Therefore, an integrative and comprehensive approach, using a set of assays for toxicity profile determination, should be adopted during ecotoxicological studies and environmental risk assessment pertaining to these elements.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 93%

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Genotoxic effect of cadmium and zinc in the peripheral erythrocytes of Prussian carp (Carassius gibelio B.)

Drąg‐Kozak

Kuchta-Gładysz²,

Grzesiakowska³

et al. 2022

Journal of Veterinary Research

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“…The ratio of the DNA in the tail region (tail intensity) is commonly used for quantifying DNA strand breakage and represents the most reliable parameter [67]. Formation of comet tails and micronuclei have been reported with several other toxicants such as hexavalaent chromium in Ctenopharyngodon idellus and Labeo rohita [68,69], Naphthalene-2-sulfonate induced toxicity in blood cells of freshwater fish Channa punctatus [70]; amoxicillin residue on zebra fish (Danio rerio) embryos (Chowdhury et al, 2020); heavy metals on Astyanax lacustris [71], sub lethal concentrations of cadmium chloride exposure in Mozambique tilapia Oreochromis Submit your Manuscript | www.austinpublishinggroup.com mossambicus [72], oil-containing drill cuttings in marine benthic goby Mugilogobius chulae [73]. Corroborative evidence from all these studies highlight the importance of comet assay as an ideal genotoxicity marker in fishes and other marine organisms.…”

Section: Discussionmentioning

confidence: 95%

Detection on Electroplating Effluent-Induced Cytopathological Alterations and DNA Damage in the Blood Samples of Cirrhinus mrigala (Hamilton, 1822)

Sudhasaravanan¹,

Binukumari²,

Rajeshkumar³

et al. 2021

austinjenvirontoxicol

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In the present study, fresh waterfish Cirrhinus mrigala has been used as a model organism to understand the mechanistic link between electroplating industrial effluent (EIE) and oxidative stress in aquatic ecosystems. To this end, the fishes were exposed to industrial effluent, in the tank water for 10, 20 and 30 days under controlled laboratory conditions. Various hematological parameters (Total RBC and WBC, Hemoglobin, MCV, MCH, PCV), biochemical metabolites (Glucose, protein, Cholesterol) and markers of genotoxicity (comet assay and micronuclear assay) were assessed. Fishes exposed for 30 days showed marked increase (p <0.001) in RBC with concomitant decrease in total WBC. There is significant (p <0.001) decline in indices of blood cells such as Hb, MCV, MCH and PCV. Results from comet assay showed significantly (p < 0.05) increased frequency genotoxicity in erythrocytes of as evident from observed increase in tail length in fishes exposed to EIE for 30 days. Overall, the results of this study, clearly demonstrated concentration dependent response of electroplating industrial effluent on freshwater fish Cirrhinus mrigala on haematological, biochemical and genotoxic markers. The results obtained herein suggests EIE as potential xenobiotics contributing towards ecotoxic and genotoxic effect in fishes.

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“…The micronucleus assay using immature bone marrow erythrocytes of mice has been widely used as a simple and sensitive shortterm screening method in-vivo for determining the mutagenicity of chemical substances [1][2][3] . As this assay uses "whole animals", it has the merits of including such factors as absorption, distribution, and metabolism of the chemical substances in the evaluation 4 .…”

Section: Introductionmentioning

confidence: 99%

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2023

IJPSR

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Isolation of plant-based pesticides gaining recent interest due to the clastogenic implications of synthetic insecticides. The polyphenolic-rich fraction from the stem bark of Streblus asper was proven as an insecticide against Dysdercus cingulatus. This study aims to evaluate the genotoxicity of polyphenolic bioinsecticide from Streblus asper (PBSA) using the mouse bone marrow micronucleus assay and compare its effect with malathion, an organophosphorus insecticide, and vepacide, a neem-based bioinsecticide. The micronucleus assay was conducted after 24 and 48 hours after the second administration of the pesticides (two doses by i.p. injection for two consecutive days). Swiss albino mice were divided into six groups, each comprising six animals. The first group received dimethyl sulphoxide (Group I -DMSO control), the second group (Group II -positive control) received 100mg cyclophosphamide/ Kg body weight, Group III received 276mg (LD 10 ) malathion/ Kg body weight and Group IV received 1000mg (LD 10 ) vepacide/ Kg body weight. Group V and VI received 500 and 1000mg polyphenolic-rich fraction (PBSA)/ Kg body weight respectively. A significantly higher frequency of micronuclei was observed in malathion and vepacide administered animals when compared to the DMSO control group whereas in the case of PBSA, no significant micronuclei formation was observed. The study concluded that polyphenolic rich fraction (PBSA) fails to influence the induction of micronuclei by proving that it has no cytogenetic toxic potential.

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Evaluation of genotoxicity, enzymatic alterations and cadmium accumulation in Mozambique tilapia Oreochromis mossambicus exposed to sub lethal concentrations of cadmium chloride

Cited by 12 publications

References 37 publications

Genotoxic effect of cadmium and zinc in the peripheral erythrocytes of Prussian carp (Carassius gibelio B.)

Genotoxic effect of cadmium and zinc in the peripheral erythrocytes of Prussian carp (Carassius gibelio B.)

Detection on Electroplating Effluent-Induced Cytopathological Alterations and DNA Damage in the Blood Samples of Cirrhinus mrigala (Hamilton, 1822)

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