Evaluation of Genotoxicity of Three Food Preservatives in Drosophila Melanogaster  Using Smart and Comet Assays

El-Hefny, ingy Maher; Hozayen, Walaa G.; Al-Senosy, Neima K.; Basal, Wesam T.; Ahmed, A.J.; Diab, Ayman A.

doi:10.15414/jmbfs.2020.10.1.38-41

Cited by 9 publications

(2 citation statements)

References 14 publications

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“…The genotoxic effect of these three food preservatives was investigated in Drosophila melanogaster by SMART and comet assay. All three food additives caused increased tumor induction and frequency in the SMART, and also induced DNA damage in the comet assay (El-Hefny et al, 2021). Potassium sorbate is used as a food preservative.…”

Section: Some Of the Genotoxicity Studies With Food Additivesmentioning

confidence: 99%

Food Additives and Genotoxicity

Altunkaynak

Yilmaz

2021

International Journal of Science Letters

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In today's changing conditions, there has been an increase in the consumption of ready-made food with the change in eating habits. Moreover, parallel to the increase in ready-made food production, there has been an increase in the food additives used. The dose amounts of food additives are determined as a result of experimental analyses. However, some additives show long-term toxic effects on the human body in genotoxicity tests. In this review, definition of substances, purposes of usage, classification, genotoxicity, definitions of tests and publications of genotoxicity studies in food additives were discussed. The search was conducted in peer-reviewed journals using Science-Direct, Web of Science and Google Scholar. In this study, genotoxicity studies conducted with food additives between 2015-2021 were complied. For this purpose, the keywords ''food additive'', ''genotoxicity'' were used together and research articles were included in this study.

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Section: Some Of the Genotoxicity Studies With Food Additivesmentioning

confidence: 99%

Food Additives and Genotoxicity

Altunkaynak

Yilmaz

2021

International Journal of Science Letters

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“…The Somatic Mutation and Recombination Test (SMART) is considered as a cheap method capable of generating reliable and reproducible results [ 16 , 17 ], besides being precise in discriminating simultaneously mutagenic, clastogenic, and/or recombinogenic agents. Moreover, it detects the genotoxicity of compounds of different chemical classes and complex mixtures, as well as aerial particles [ 18 , 19 , 20 ]. The test for the detection of epithelial tumor (ETT) is widely used for the evaluation of carcinogenic or anticarcinogenic activity in an economic, fast, and sensitive way to different treatments.…”

Section: Introductionmentioning

confidence: 99%

Copper(II) Complex Containing 4-Fluorophenoxyacetic Acid Hydrazide and 1,10-Phenanthroline: A Prostate Cancer Cell-Selective and Low-Toxic Copper(II) Compound

et al. 2022

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Prostate Cancer (PCa) is the second leading cause of cancer-related deaths among men worldwide. The treatment of advanced cases is based on chemotherapy, which lacks specificity and efficacy, due to severe side effects and resistance to the traditional drugs. Copper complexes have shown antitumoral efficacy and low toxicity, being considered a promising class of metal-based drugs for the treatment of malignant neoplasms. Thus, the present study aimed to evaluate the cellular effects of a copper(II) complex with 4-fluorophenoxyacetic acid hydrazide and 1,10-phenanthroline (1) on PCa cell lines, as well as the mutagenic/recombinogenic and anticarcinogenic potential of 1 in Drosophila melanogaster. PNT-2 (non-tumorigenic), LNCaP (hormone-responsive PCa) and PC-3 (androgen-independent PCa) cells were cultured, and cytotoxicity was assessed using the MTT assay. The expression levels of the proliferation markers Ki-67 and Cyclin D1 were analyzed by flow cytometry. Furthermore, the Somatic Mutation and Recombination Test (SMART) and the Epithelial Tumor Test (ETT) were performed. Complex 1 was selective to LNCaP cells, significantly reducing Ki-67 and Cyclin D1 expression levels. Sub-toxic concentrations of complex 1 were defined by the toxicity test in D. melanogaster, and no mutagenic/recombinogenic/carcinogenic effects were observed. Anticarcinogenic potential was observed in D. melanogaster, suggesting modulating activity of the complex 1 against Doxorubicin, a drug used as control by its carcinogenic properties. Therefore, complex 1 is a possible starting point for the development of new antitumor agents for the treatment of PCa.

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Analysis of genotoxic effects of food preservatives sodium acetate (E262) and sodium sulfite (E221) in human lymphocytes

Altunkaynak,

Avuloglu-Yilmaz

2024

Food Sci Biotechnol

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Evaluation of Genotoxicity of Three Food Preservatives in Drosophila Melanogaster Using Smart and Comet Assays

Cited by 9 publications

References 14 publications

Food Additives and Genotoxicity

Food Additives and Genotoxicity

Copper(II) Complex Containing 4-Fluorophenoxyacetic Acid Hydrazide and 1,10-Phenanthroline: A Prostate Cancer Cell-Selective and Low-Toxic Copper(II) Compound

Analysis of genotoxic effects of food preservatives sodium acetate (E262) and sodium sulfite (E221) in human lymphocytes

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