Evaluation of Gliadin Antibodies for Detection of Coeliac Disease

Kilander, Anders; Dotevall, G; Fällström, S. P.; Gillberg, R; Nilsson, Lars‐Åke; Tarkowski, Andrzej

doi:10.3109/00365528309181610

Cited by 75 publications

(36 citation statements)

References 11 publications

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“…Antigliadin (IgA and IgG) antibodies were assessed by a modified enzymelinked immunosorbent assay (DIG-ELISA) as described elsewhere (Kilander et al, 1983;Lindberg et al, 1985). Antiendomysial antibodies of the IgA type (EmA) were detected by the indirect immunofluorescence technique using monkey oesophagus as the substrate (Ascher et al, 1996).…”

Section: Histopathologymentioning

confidence: 99%

Adult coeliac patients do tolerate large amounts of oats

et al. 2003

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Objective: The aim of the present study was to investigate whether adult patients with coeliac disease in remission could include large amounts of oats in their daily gluten-free diet for an extended period of time without adverse effects. Design, subjects and methods: Twenty adult coeliac patients in remission included large amounts of uncontaminated rolled oats in their daily diet for a prolonged period. The examinations, performed four times during the study period, included small bowel endoscopy with biopsies, blood samples (nutritional status, serological analysis), height and body weight, gastrointestinal symptoms and dietary records. Gastrointestinal symptoms and diet were also investigated through unannounced telephone interviews once a month during the study period. Results: No adverse effects of a large intake of oats were seen in small bowel histology, serology nor in nutritional status in the 15 subjects who completed the whole study period. Two of the subjects dropped out because of gastrointestinal symptoms and three for non-medical reasons. The median intake of oats was 93 g=day and the compliance to the oat diet was found to be good. Examinations of the patients after drop-out did not show any deterioration in small bowel histology or nutritional status nor raised levels of antibodies. Conclusion: Results from this study indicate that adult patients with coeliac disease in remission can include large amounts of controlled wheat-free rolled oats for an extended period of time without adverse effects.

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Section: Histopathologymentioning

confidence: 99%

Adult coeliac patients do tolerate large amounts of oats

et al. 2003

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“…The main objective of this study was to In the last two decades, the determination of serum antibodies has proved to be a useful tool for diagnosis and follow-up of patients on a gluten-free diet (2,3,(18)(19)(20)(21). Nevertheless, most of the studies refer to populations from developed countries, raising questions about the utility of these tests in nondeveloped countries, where the high frequency of so-called ambient enteropathies may produce false-positive results for antigliadin antibodies (10).…”

Section: Discussionmentioning

confidence: 99%

Serum antigliadin antibody levels as a screening criterion before jejunal biopsy indication for celiac disease in a developing country

Bahia

Rabello²,

Filho

et al. 2001

Braz J Med Biol Res

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The objective of the present study was to determine the efficacy of detection of antigliadin immunoglobulins G and A (IgG and IgA) for the diagnosis of celiac disease in a developing country, since other enteropathies might alter the levels of these antibodies. Three groups were studied: 22 patients with celiac disease (mean age: 30.6 months), 61 patients with other enteropathies (mean age: 43.3 months), and 46 patients without enteropathies (mean age: 96.9 months). Antigliadin IgG and IgA ELISA showed sensitivity of 90.9 and 95.5%, respectively. With the hypothetical values of prevalence ranging from 1:500 to 1:2000 liveborns, the positive predictive value varied from 8.5 to 2.3% for IgG and from 4.8 to 1.1% for IgA. Considering the patients without enteropathies, specificity was 97.8 and 95.7% for IgG and IgA, respectively. In patients with other enteropathies, specificity was 82.0 and 84.1%, respectively. When patients with and without other enteropathies were considered as a whole, specificity was 88.8 and 91.6%, respectively. The specificity of positive IgG or IgA was 93.5% in children without enteropathies and 78.7% in the presence of other enteropathies. The negative predictive value for hypothetical prevalences varying from 1:500 to 1:2000 liveborns was 99.9%. Thus, even in developing countries where the prevalence of non-celiac enteropathies is high, the determination of serum antigliadin antibody levels is a useful screening test prior to the jejunal biopsy in the investigation of intestinal malabsorption.

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“…However, since mucosal involvement is quite variable in each patient, ranging from minimal changes to severe extensive damage, we suppose that this variability is responsible for the wide range of PHD values in CrD patients. The association of the serum antigliadin anti body assay [17] to the PHD test likely im proves discrimination between patients with CD and CrD.…”

Section: Discussionmentioning

confidence: 99%

Postheparin Plasma Diamine Oxidase in Subjects with Small Bowel Disease

D’Agostino¹,

Daniele²,

Pignata³

et al. 1988

Digestion

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Diamine oxidase (DAO) is an enzyme located almost exclusively in the villus tip enterocytes of mammals. Its plasma activity, normally very low, is enhanced by intravenous heparin that releases the enzyme from the enterocytes into the blood. Postheparin plasma DAO (PHD) values have been shown to be significantly reduced in patients with malabsorption and villus atrophy and in patients with Crohn’s disease, thus suggesting that this test explores the mucosal integrity. The execution of the PHD test requires 8 blood samples over a period of 120 min after an intravenous injection of 15,000 IU of heparin, and then the calculation of the area under the curve. The aim of this study was to simplify the test and make it more acceptable by choosing only one of the eight curve points required for the calculation of the area under the curve and then assessing its discriminant power in different small bowel diseases. To this end, a discriminant analysis was performed on PHD curves of 16 normal subjects, 25 patients with celiac disease, 14 patients with treated celiac disease, 5 patients with diffuse primary small bowel lymphoma, 4 patients with small bowel lymphoma during treatment, and 55 patients with small bowel Crohn’s disease. Plasma DAO values assayed 1 h (T60) after the injection of 15,000 IU of heparin proved to be the best discriminator curve point and the use of the T60 point alone may be usefully employed instead of the area under the 120-min curve. The simplified test is also useful in monitoring the recovery of the small bowel enterocytic mass in patients with celiac disease and small bowel lymphoma during treatment.

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Evaluation of Gliadin Antibodies for Detection of Coeliac Disease

Cited by 75 publications

References 11 publications

Adult coeliac patients do tolerate large amounts of oats

Adult coeliac patients do tolerate large amounts of oats

Serum antigliadin antibody levels as a screening criterion before jejunal biopsy indication for celiac disease in a developing country

Postheparin Plasma Diamine Oxidase in Subjects with Small Bowel Disease

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