Evaluation of Gold Nanoparticles Coated Sandwich Elisa as Innovative Diagnosis for Human Bancroftian Filariasis

El-Shafey, Omaima K.; Afifi, Amira F.; Oshiba, Salwa F.; Aly, Ibrahim; Lasheen, Marwa E.

doi:10.21608/jesp.2018.77505

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…The advantage of Nano-based ELISA over traditional ELISA in diagnosis of different parasitic infections was previously elucidated by several studies (Moharm et al 2014; Naser et al 2017; El-Shafey et al 2018; Khodadadi et al 2021). This promising high performance of Nano-based ELISA was explained by many studies.…”

Section: Discussionmentioning

confidence: 99%

Real-time PCR versus traditional and Nano-based ELISA in early detection of murine trichinellosis

et al. 2023

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Trichinellosis is a serious foodborne zoonosis. It poses a serious risk to public health worldwide. Early serological diagnosis of trichinellosis is influenced by an immunological ‘silent’ phase following infection. This highlights the necessity for developing sensitive diagnostic approaches to be employed when antibodies cannot be detected. In this work, the validity of traditional ELISA, Nano-ELISA and real time polymerase chain reaction (PCR) were evaluated in early diagnosis of Trichinella spiralis. Swiss albino mice were orally infected with 100 and 300 muscle larvae/mouse. Mice were sacrificed 4, 6, 8, 10, 15, and 28 days post-infection (dpi). Blood samples were tested for circulating antigen by traditional ELISA and Nano-ELISA using anti-rabbit polyclonal IgG conjugated with AgNPs and for Rep gene by SYBR green real-time PCR. Rep gene detection by SYBR green real-time PCR could detect T. spiralis with 100% sensitivity in the mild infection group at 8 dpi, while in the severe infection group it reached 100% sensitivity at 4 dpi. Nano-ELISA could detect T. spiralis circulating antigen from 4 dpi in both mild and severe infection and reached 100% sensitivity at 8 dpi and 6 dpi in mild and severe infection, respectively. However, traditional ELISA could detect T. spiralis circulating antigen from 6 dpi and reached maximum sensitivity at 15 dpi in the mild infection group, while in the severe infection group detection began at 4 dpi and reached 100% sensitivity at 8 dpi. Nano-ELISA and real time PCR, using Rep gene, are useful tools for the detection of early T. spiralis infection even in its mild infection state.

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Section: Discussionmentioning

confidence: 99%

Real-time PCR versus traditional and Nano-based ELISA in early detection of murine trichinellosis

et al. 2023

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Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA)

Gomaa

2020

Experimental Parasitology

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Vector-Borne diseases in Egypt

Eassa

El‐Wahab

2022

Journal of Vector Borne Diseases

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Vector borne diseases (VBDs) remain one of the greatest dangers to global health. At least seven VBDs of public health concern are prevalent in Egypt, including schistosomiasis, fascioliasis, lymphatic filariasis, leishmaniasis, malaria, dengue, and Rift Valley fever. Although many of these diseases are preventable by using evidence-based protective measures, VBD expansion patterns over the past few decades pose a significant challenge for modern parasitology and tropical medicine. In their action plan, Egypt did not identify populations at risk of VBDs. Egypt intends to improve its regional and international communication to identify pathogens and infections and develop “One Health”- compliant preparedness and prevention strategies. However, cross-border collaborations are required for the control of VBDs. In this context, we provide a situational analysis and comprehensive review of the epidemiological data on Egypt’s most prevalent VBDs based on an exhaustive search of the major electronic databases and literature from 1950 to 2019. We identified the gaps in Egypt’s preparedness for vector-borne disease threats, including adaptation documents, surveillance and monitoring, environmental management, and preparations for the health system. There is a lack of implementation of an integrated vector management strategy that integrates chemical, environmental, and biological control as well as health education. This necessitates cross-sectoral coordination and community involvement to improve vector control activities and the use, storage, and disposal of pesticides.

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Evaluation of Gold Nanoparticles Coated Sandwich Elisa as Innovative Diagnosis for Human Bancroftian Filariasis

Cited by 3 publications

References 6 publications

Real-time PCR versus traditional and Nano-based ELISA in early detection of murine trichinellosis

Real-time PCR versus traditional and Nano-based ELISA in early detection of murine trichinellosis

Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA)

Vector-Borne diseases in Egypt

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