Evaluation of goose serum amyloid a acute phase response by enzyme-linked immunosorbent assay

Abstract

Serum amyloid A (SAA) is of interest as the circulating precursor of amyloid A protein, the fibrillar component of AA (secondary) amyloid deposits, and also as an extremely sensitive and rapid major acute phase protein. Serum concentrations of acute phase proteins (APPs) provide valuable information about the diagnosis and prognosis of various diseases, and thus the relevance of APPs for monitoring the health status of domestic animals is widely accepted. More importantly, the measurement of SAA concentration assists in assessing the prognosis in secondary amyloidosis, which is a common disease of geese, affecting an increasing number of animals. In the present study we introduce a highly sensitive goose-specific ELISA method for measuring SAA concentration in goose serum or plasma samples. Samples were taken from geese of the Landes Grey and Hungarian White breeds, which were stimulated for an acute phase reaction by administration of a commercially available fowl cholera vaccine containing inactivated Pasteurella multocida. Strong and characteristically rapid acute phase responses were measured in both breeds, peaking at approximately 24 h after inoculation. The maximum SAA concentration was 1200 µg/ml. At 72 h postinoculation, the concentrations returned to pre-inoculation values. There was significantly (p = 0.004) less intense response in the control groups; however, a very mild increase of SAA levels was detected due to the stress inevitably caused by the sampling procedure.

Temporal Effects of High Fishmeal Diet on Gut Microbiota and Immune Response in Clostridium perfringens-Challenged Chickens

“…Moreover, Chamanza et al (1999) demonstrated that perhaps SAA is a reliable APP for diagnosing inflammatory lesions in chickens. The expression of SAA also could be increased by bacterial and viral infection such as with Pasteurella multocida , Staphylococcus aureus and infectious bronchitis virus ( Chamanza et al, 1999 ; Kovacs et al, 2007 ; Asasi et al, 2013 ). In our study, C. perfringens challenge with a high fishmeal diet, rather than infection alone, resulted in a higher intestinal lesion score and increased the relative abundance of C. perfringens at 1 dpi that was accompanied by an increase in AGP and SAA increase in liver.…”

Evaluation of sialic acid and acute-phase proteins (haptoglobin and serum amyloids A) in healthy and avian infection bronchitis virus-infected chicks

“…Tracheitis, bronchitis, tracheal oedema, bronchial caseous plugs and air sacculitis indicates widespread inflammatory reaction in IBV this causes release and elevation of SAA and Hp concentrations. Kovacs et al (2007) showed a mild increase in SAA in the goose by administration of a fowl cholera vaccine containing inactivated Pasteurella multocida. Vaccination was an inflammatory factor and produced increased SAA levels.…”

Diagnostic value of serum amyloid A in differentiating the inflammatory disorders in wild birds

“…In particular, C-reactive protein (CRP) in dogs and serum amyloid A (SAA) in cats have been routinely tested in small animal clinics as measurement kits have been commercialized. Known avian APPs include SAA, alpha-1 acid glycoprotein, ceruloplasmin, transferrin, fibrinogen, and haptoglobin (8)(9)(10)(11)(12)(13)(14)(15). Among these avian APPs, SAA has been the focus of research because it is a precursor of the amyloid A (AA) protein.…”