3273ReseaRch M aize (Zea mays L.) stover is harvested for a variety of uses, including as a feedstock for cellulosic ethanol production or for use in livestock operations as bedding or feed. The use of 136 billion L yr −1 of renewable fuels by 2022 is mandated by the Renewable Fuel Standard as established in the Energy Independence and Security Act of 2007, of which cellulosic biofuels would comprise 61 billion L (USDOE, 2011). The promulgated rules identify maize stover as a cellulosic biofuels feedstock (Schnepf, 2013), thus expanding long-term opportunities for stover as an additional revenue stream in the renewable fuels arena. Other uses also enhance the economic incentive to harvest maize stover. For example, chemical processing of residues can increase digestibility by 35 to 62% by decomposition of lignocellulosic bonds (Shreck et al., 2011), making maize stover more attractive for use as a livestock feed, especially when grain prices escalate (Meteer, 2014). The increased harvest of maize stover, however, compounds the already hefty challenges of natural resources conservation in conventional systems.Maize stover provides myriad ecosystems services, and returning stover to soil recycles plant nutrients. Standard fertilization practices can be insufficient to compensate for nutrient loss after residue removal or when soil erosion approaches the soil loss ABSTRACT The Renewable Fuels Standard mandate provides enhanced opportunity for maize (Zea mays L.) stover use as a bioenergy feedstock. Living mulch (LM) offers a possible solution for the natural resources constraints associated with maize stover biomass harvest. A two-site-year study was conducted near Boone and Kanawha, IA, in both maize following maize (MM) and maize following soybean [Glycine max (L.) Merr.] (SM) sequences to evaluate the impact of established and chemically suppressed Kentucky bluegrass (Poa pratensis L.) 'Ridgeline', 'Wild Horse', 'Oasis', and 'Mallard' blend and creeping red fescue (Festuca rubra L.) 'Boreal' as LM on three maize hybrids (population sensitive, population insensitive, and yield stable). Maize grain yield for the no LM treatments in the MM and SM sequences was 12.0 and 13.2 Mg ha −1 , respectively, at Boone and 12.8 and 14.8 Mg ha −1 , respectively, at Kanawha, 23 to 73% greater than the LM treatment. Ethanol yield (L ha −1 ) was 12 to 119% greater, protein concentration was £9% greater, and starch concentration was £1% lower in the no LM treatment maize than in LM treatment maize. Maize hybrid by cover interaction was significant for parameters including total aboveground biomass and protein concentration at Boone, with inconsistent maize hybrid responses to the LM system. Stover yield, stover quality, stover C and N, leaf area index, maize plant density, maize maturity, and sequence year in the MM sequence were also evaluated. Results emphasize the need for maize hybrid and LM system compatibility, as well as effective LM suppression techniques.