A juneberry (Amelanchier alnifolia) cultivar trial was conducted to evaluate fruit yield, quality, and other characteristics for juneberry cultivars and a native biotype. One-year old micropropagated material was transplanted and established in North Dakota in 2004. The native biotype is available as a conservation plant from Towner State Nursery (Towner, ND) and was included as a readily available juneberry for producers. Fruit diameter, soluble solids content, yield (total and marketable), and plant size measurements were taken during the 2010 and 2011 season. ‘Martin’, the native biotype, ‘Parkhill’, ‘Pembina’, ‘Regent’, and ‘Thiessen’ produced the highest total yield in 2010, whereas ‘Parkhill’ had the highest total yield in 2011, followed by ‘Thiessen’ and then ‘Martin’. Cultivars Martin, Parkhill, and Thiessen produced the highest marketable yield over the 2-year study. ‘Martin’ and ‘Thiessen’ fruit were larger and heavier than the rest of the cultivars. The largest plants were ‘Martin’, ‘Parkhill’, ‘Regent’, and ‘Thiessen’. Soluble solids concentrations were similar among all cultivars. Cultivars Martin or Thiessen should be recommended to commercial producers wanting a high yielding cultivar with uniform fruit ripening, whereas Parkhill should be recommended to producers with a you-pick operation wanting a high yielding cultivar with an extended fruit ripening period.