Evaluation of Haematopoietic Cells and M/E Ratio in the Bone Marrow of the Partridge (Alectoris chukar)

Tadjalli, M.; Nazifi, Saeed; Haghjoo, Rahil

doi:10.3923/ijps.2012.23.27

Cited by 3 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Glick (1987) showed that cellular composition is constant in chicken femur and tibiotarsus, but with increasing age, the numbers of immature granulocytes decreased. Based on light microscopy evaluation, the cellular composition of the bone marrow from various avian species was described, including chicken ( Glick, 1987 ), partridge ( Tadjalli et al, 2012 ), duck ( Tadjalli et al, 1997 ), black-headed gull ( Tadjalli and Hadipoor, 2002 ), pheasant ( Tadjalli et al, 2013 ), Japanese quail ( Nazifi et al, 1999 ), and parrots ( Schwartz et al, 2019 ).…”

Section: Hematopoietic Stem Cells and Hematopoiesismentioning

confidence: 99%

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

Zmrhal

Svoradová

Bátik

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Three-dimensional (3D) cell culture is attracting increasing attention today because it can mimic tissue environments and provide more realistic results than do conventional cell cultures. On the other hand, very little attention has been given to using 3D cell cultures in the field of avian cell biology. Although mimicking the bone marrow niche is a classic challenge of mammalian stem cell research, experiments have never been conducted in poultry on preparing in vitro the bone marrow niche. It is well known, however, that all diseases cause immunosuppression and target immune cells and their development. Hematopoietic stem cells (HSC) reside in the bone marrow and constitute a source for immune cells of lymphoid and myeloid origins. Disease prevention and control in poultry are facing new challenges, such as greater use of alternative breeding systems and expanding production of eggs and chicken meat in developing countries. Moreover, the COVID-19 pandemic will draw greater attention to the importance of disease management in poultry because poultry constitutes a rich source of zoonotic diseases. For these reasons, and because they will lead to a better understanding of disease pathogenesis, in vivo HSC niches for studying disease pathogenesis can be valuable tools for developing more effective disease prevention, diagnosis, and control. The main goal of this review is to summarize knowledge about avian hematopoietic cells, HSC niches, avian immunosuppressive diseases, and isolation of HSC, and the main part of the review is dedicated to using 3D cell cultures and their possible use for studying disease pathogenesis with practical examples. Therefore, this review can serve as a practical guide to support further preparation of 3D avian HSC niches to study the pathogenesis of avian diseases.

show abstract

Section: Hematopoietic Stem Cells and Hematopoiesismentioning

confidence: 99%

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

Zmrhal

Svoradová

Bátik

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…However, there is limited information on the changes in bone marrow in PCV-2 infection. Bone marrow is widely studied for haematopoietic and mesenchymal cells morphologies (Tadjalli et al, 2013). Alteration in the cytology of myeloid and erythroid progenitor cells usually happens in viral infections.…”

mentioning

confidence: 99%

Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs

Saifudeen¹

2021

J Vet Anim Sci

View full text Add to dashboard Cite

Porcine circovirus associated diseases (PCVAD) caused by porcine circovirus type-2 (PCV-2) are emerging viral diseases with unfavourable effects on animal health and swine economy. We have a lot of information regarding the changes in the lymphoid organs and spleen in PCV-2 infected pigs whereas the reason for anaemic changes in the carcasses and the pathological effects of PCV-2 in bone marrow are still not well studied. Hence, an extensive study to identify the changes in myeloid and erythroid cells of bone marrow in PCV-2 infected pigs was carried out. Myeloid and erythroid series of cells were counted and analysed from the freshly collected bone marrow cytological smears from the PCV-2 suspected samples. Later, PCV-2 infection was confirmed by polymerase chain reaction (PCR) and characteristic histopathological findings. The PCR yielded an amplicon of ~ 481 bp product and those positive cases were selected for determining the Myeloid to Erythroid ratio (M : E ratio). However, values did not significantly differ in any of the cellular components between PCV-2 positive animals and PCV-2 negative animals which indicated that the bone marrow was not the specific target organ for PCV-2 viral infections. However, increased lympho-histiocytic and plasmacytic infiltration was noticed in both lymphoid and non-lymphoid organs. These characteristic features of PCV-2 infection could be considered as a major reason for increased proliferation of myeloid cells.

show abstract

Hematology of Galliformes

Piccione¹,

Hokamp²

2022

Schalm's Veterinary Hematology

View full text Add to dashboard Cite

Evaluation of Haematopoietic Cells and M/E Ratio in the Bone Marrow of the Partridge (Alectoris chukar)

Cited by 3 publications

References 11 publications

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs

Hematology of Galliformes

Contact Info

Product

Resources

About