Evaluation of halothane sensitivity and prediction of post-mortem muscle metabolism in pigs from a muscle biopsy using 31P NMR spectroscopy

Lahučký, R.; Mojto, J.; Poltarsky, J.; Miri, A.; Renou, Jean-Pierre; Talmant, André; Monin, Gabriel

doi:10.1016/0309-1740(93)90008-6

Cited by 24 publications

(9 citation statements)

References 7 publications

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“…Out of a number of spectra for calculation only the fi rst and second were used. The levels of the individual phosphorus compounds (SP, Pi, PCr, ATP) were expressed as a percentage of the total content of phosphorus compounds as described previously (Lahucky et al, 1993(Lahucky et al, , 2000 and the ratio of PCr/Pi was calculated.…”

Section: Chemical Analysismentioning

confidence: 99%

“…Increasing and maintenance the PCr/Pi ratio and thus the amount of available energy for resynthesis of ATP could also improve some meat quality parameters, as indicated by studies of the metabolism of phosphorus compounds using phosphorus nuclear magnetic resonance spectroscopy, both ante mortem (Lahucky et al, 1993(Lahucky et al, , 2002Kohn et al, 1999) and post mortem (Miri et al, 1992;Shen et al, 1992;Moesgaard et al, 1995;Scholz et al, 1995;Lahucky et al, 2000;Bertram et al, 2002). Muscle metabolism around the time of slaughter has a major eff ect on pork quality.…”

mentioning

confidence: 99%

“…Muscle metabolism around the time of slaughter has a major eff ect on pork quality. An important genetic factor that alters muscle metabolism is the presence of mutations in the ryanodine receptor gene (RYR1) as was shown in many studies (Lahucky et al, 1993(Lahucky et al, , 2002Fiedler et al, 1999;Maddock et al, 2002). Carriers (heterozygotes, Nn) produce lower quality meat and it was proposed that carriers should be excluded from the breeding population (and also from welfare point of view).…”

mentioning

confidence: 99%

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Effects of creatine and vitamin E on muscle energetic metabolism, antioxidant stability and meat quality of pigs

Lahučký¹,

Bučko²,

Haščík³

et al. 2013

Acta Univ. Agric. Silvic. Mendelianae Brun.

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LAHUČKÝ, R., BUČKO, O., HAŠČÍK, P., LIPTAJ, T.: Eff ects of creatine and vitamin E on muscle energetic metabolism, antioxidant stability and meat quality of pigs. Acta univ. agric. et silvic. Mendel. Brun., 2012, LX, No. 5, pp. 151-160 The eff ects of supplementing the diet of pigs with creatine monohydrate (CMH) and vitamin E on blood plasma creatine concentration, vitamin E level in muscle, muscle energy metabolism, antioxidant capacity and meat (musculus longissimus dorsi) quality of pigs (DNA tested and negative on malignant hyperthermia) were investigated. Two treatments were used: supplementation with CMH alone (2 g.kg −1 of feed, 10 days before slaughter); and supplementation with both vitamin E (500 mg -tocopherol.kg −1 of feed for minimum of 30 days) and CMH (2 g.kg −1 of feed, 10 days before slaughter). Pigs supplemented with CMH alone showed elevated plasma creatine concentration (P ≤ 0.05). Phosphorus nuclear magnetic resonance ( 31 P NMR) measurements on post mortem (15 min.) muscle samples showed the highest phosphocreatine levels and ratio PCr/Pi (P ≤ 0.05) in CMH supplemented pigs alone and in combination with vitamin E. Dietary supplementation with vitamin E signifi cantly (P ≤ 0.05) increased the concentration of -tocopherol in meat. Supplementation with CMH alone or in combination with vitamin E resulted in higher (P = 0.07) a values of loin chops at 5 days of storage. Antioxidative capacity (measured as MDA production a er incubation of longissimus muscle homogenates with Fe 2+ /ascorbate) was substantially improved by vitamin E and somewhat by CMH supplementation. meat quality, pork, creatine monohydrate, vitamin E Previous research has examined the eff ects of supplementing swine fi nishing diets with creatine.

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Section: Chemical Analysismentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Effects of creatine and vitamin E on muscle energetic metabolism, antioxidant stability and meat quality of pigs

Lahučký¹,

Bučko²,

Haščík³

et al. 2013

Acta Univ. Agric. Silvic. Mendelianae Brun.

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“…In pork, the 31 P-NMR spectroscopy revealed that the high phosphomonoesters and low phosphocreatinine concentrations associate with PSE-prone meat, while high inorganic phosphate and low phosphomonoesters indicate a DFD-prone meat (Miri et al 1992). 31 P-NMR was also extensively used to show that the pigs with malignant hyperthermia syndrome suffer from impaired muscle metabolism resulting in poor meat quality traits (Lahucky et al 1993, Moesgaard et al 1995. In the muscleto-meat conversion process in carcasses, the rate of pH decline is reflected by the rate of glycolysis among other characteristics such as animal species, preslaughter handling, and postmortem temperature (Bertram et al 2002c, Jones et al 1993, Josell et al 2000.…”

Section: Metabolomics Of Tendernessmentioning

confidence: 99%

“…In most of the earlier studies on meat tenderness, 31 P-NMR spectroscopy was mostly employed to obtain the information on the levels of phosphorylated metabolites and their postmortem changes in meat muscles from beef, pork, and lamb (Lahucky et al 1993, Lundberg et al 1987, Miri et al 1992, Moesgaard et al 1995, Vogel et al 1985. This is because of the fact that under the anaerobic condition following slaughter, replenishment of the cell energy (adenosine triphosphate [ATP] from adenosine diphosphate [ADP]) occurs primarily via degradation of glycogen (glycolysis), sugar phosphates such as glucose-6-phosphate (G-6-P), and from the use of creatine phosphate.…”

Section: Metabolomics Of Tendernessmentioning

confidence: 99%