Evaluation of hematin-catalyzed Orange II degradation as a potential alternative to horseradish peroxidase

Córdoba, Agostina; Magario, Ivana; Ferreira, Marı́a Luján

doi:10.1016/j.ibiod.2012.05.020

Cited by 17 publications

(15 citation statements)

References 80 publications

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“…This can result in major local H 2 O 2 concentration in the vicinity of the catalytic site. The relative increase in H 2 O 2 to phenolic substrate concentration can generate two main routes of reaction: the porphyrin inactivation by reactions with excess of H 2 O 2 , or a catalatic‐like pathway with O 2 formation . In previous studies of ARS decolorization, we observed the release of O 2 during hematin homogeneous catalysis .…”

Section: Resultsmentioning

confidence: 78%

“…Optimal decolorization conditions for each dye were evaluated in previous work and maintained in the present study. Reaction conditions are detailed in Table .…”

Section: Methodsmentioning

confidence: 99%

“…A preliminary study on the comparison of hematin and HRP was carried out but the biomimetic catalyst needed to be optimized . Azo and anthraquinone dyes have been successfully decolorized by enzymatic and biomimetic homogeneous systems at alkaline pH by our group . Aspects of hematin reaction mechanism have been discussed in these works.…”

Section: Introductionmentioning

confidence: 99%

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Modified chitosan as an economical support for hematin: application in the decolorization of anthraquinone and azo dyes

Córdoba

Magario

Ferreira

2014

J. Chem. Technol. Biotechnol.

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BACKGROUND: Azo and anthraquinone dyes have been successfully decolorized using enzymatic and biomimetic homogeneous systems. 1,2 Hematin, a horseradish peroxidase (HRP) biomimetic, immobilized on chitosan via glutaraldehyde coupling was able to decolorize Alizarin Red S and Orange II solutions. A Doehlert experimental design and response surface analysis was applied to determine conditions for optimal mass of catalyst and catalytic efficiency of the immobilization procedure. RESULTS:The catalysts with supported hematin showed 33% activity (relative to homogeneous hematin as 100%) in decolorization reactions. After six reuses the catalytic activity was maintained at 60% of the initial one. Hematin anchoring to chitosan without alteration of the iron-porphyrin ring was confirmed by ICP, FTIR and UV/visible spectrophotometric methods. In addition, the effect on hematin activity in the decolorization of aminopropyltriethoxysilane (APTS) as a 'spacer arm' between hematin-glutaraldehyde and chitosan was studied.CONCLUSIONS: An economical heterogeneous catalyst alternative to HRP has been obtained. The spacer arm improves the catalyst́s performance with activities as high as 57% relative to homogeneous hematin. These results support the notion that the activity loss of supported hematin is caused by the restricted access to Fe of the bulky phenolic dyes.

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Section: Resultsmentioning

confidence: 78%

“…Optimal decolorization conditions for each dye were evaluated in previous work and maintained in the present study. Reaction conditions are detailed in Table .…”

Section: Methodsmentioning

confidence: 99%

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Modified chitosan as an economical support for hematin: application in the decolorization of anthraquinone and azo dyes

Córdoba

Magario

Ferreira

2014

J. Chem. Technol. Biotechnol.

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“…Different bacterial strains were used by Bhunia et al to degrade acid, direct, and reactive textiles dyes and the highest removals were obtained in the pH range of 5.5 to 10. An evaluation of the colour removal from solutions of the textile dye Orange II by horseradish peroxidase (HRP) was performed by Córdoba et al using the response surface methodology. These authors reported that the conversions were most strongly affected by the pH followed by the catalyst and peroxide concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…Horseradish peroxidase (HRP) is a peroxidase enzyme widely used to degrade recalcitrant organic compounds, such as dyes, phenol, and aromatic compounds, primarily because it maintains stability under a wide range of temperature and pH conditions . The enzyme HRP removed more than 92 % of the colour from solutions containing the textile dye Orange II after 60 min …”

Section: Introductionmentioning

confidence: 99%

Enzymatic reuse of simulated dyeing process effluent using horseradish peroxidase

et al. 2017

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Reactive dyes are complex structures that cause environmental damage when discarded. The aim of this study was to evaluate the reuse of effluent from an enzyme‐catalyzed dyeing process. The main parameters that influence the degradation of the mixture of dyes using the enzyme horseradish peroxidase were studied using response surface methodology: enzyme, hydrogen peroxide, and dye concentrations as well as the pH. The best conditions for the synthetic effluent were then applied to the reuse of the effluent from the dyeing process in laboratory and pilot scales. In the studies in pilot scale a high colour intensity was obtained (ΔE* value of 0.6) and a good washing fastness (4.0). The results obtained indicate that peroxidases could be used for colour removal on an industrial scale to save water and energy in the post‐dyeing process, reusing part of this effluent with the same efficiency as that of a traditional process.

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Immobilization of horseradish peroxidase onto electrospun polyurethane nanofiber matrices

Urrea

Caracciolo

Haure

et al. 2021

Polymers for Advanced Techs

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In this work, horseradish peroxidase (HRP) was immobilized onto polyurethane nanofiber membranes. The following variables were optimized to maximize the surface density of grafted HRP (Q): NaClO functionalization time (tf), immobilization time (ti), HRP concentration of the immobilization solution (CHRP), and immobilization temperature (T). Catalytic activity was evaluated using the decolorization reaction of Orange II (OII). A statistical analysis of SEM images demonstrates that fiber diameters (d) of native (M), AGE‐modified (MA), and HRP immobilized (MAH) electrospun membranes obeyed a log‐normal distribution and that the effects of the NaClO activation procedure, and the use of MAH membranes in OII oxidation during 4 h on the fiber size were negligible. Although obtained membranes at 40°C (MAH9540) presented the highest Q, matrices obtained at 20°C (MAH9520) exhibited the highest catalytic activity, indicating that HRP was partially inactivated during the immobilization at 40°C. Reusability tests demonstrated that membranes retained between 11% and 33% of their initial enzyme activity after a total reaction time between 4 and 8 h.

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Evaluation of hematin-catalyzed Orange II degradation as a potential alternative to horseradish peroxidase

Cited by 17 publications

References 80 publications

Modified chitosan as an economical support for hematin: application in the decolorization of anthraquinone and azo dyes

Modified chitosan as an economical support for hematin: application in the decolorization of anthraquinone and azo dyes

Enzymatic reuse of simulated dyeing process effluent using horseradish peroxidase

Immobilization of horseradish peroxidase onto electrospun polyurethane nanofiber matrices

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