Evaluation of hepatocellular and Kupffer cell function of the isolated guinea pig liver perfused by a fluorocarbon medium

Roeser, H. P.; Cooksley, W. G. E.; Mack, S

doi:10.1016/0016-5085(78)90798-9

Cited by 6 publications

(2 citation statements)

References 11 publications

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“…The oxygen-carrying compound fluorocarbon was used as the perfusion medium because of its stability (Dixon and Holland, 1975). It was prepared by a method (Roeser. Cooksley and Mack, 1978) based on Ihal described by Krone el al.…”

Section: Methodsmentioning

confidence: 99%

Portal Vein Perfusion of the Isolated Rat Liver: Some Markers of Hyperthermic Liver Damage

Wynne

Mack

McRae

et al. 1984

Aust J Exp Biol Med

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Summary.The cytoloxlc efTects of hyperthermia on tumours are readily measured. l';iramelers of injury to normal tissues are needed to evaluate therapeulic potential.Fifty-five isolated rat livers were perfused in vitro for 180 min in order to determine parameters of hyperthermic injury. During this period they were heated for I h at lemperaiures ranging from 37° to 45° and then assessed for evidence of hyperthermic injury.The most critical indicator of hyperthcrmic injury was sustained depression of bile production. Bile sccreiion decreased by 80% after heating at temperatures above 42°. A^partate amino transferase (AST) release increased significantly at temperatures of 42° and above.Potassium and variable amounts of glucose were released into the medium during heating, reflecting temporary changes in melabolism at high temperatures.

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Section: Methodsmentioning

confidence: 99%

Portal Vein Perfusion of the Isolated Rat Liver: Some Markers of Hyperthermic Liver Damage

Wynne

Mack

McRae

et al. 1984

Aust J Exp Biol Med

View full text Add to dashboard Cite

show abstract

“…Perfluorochemical erythrocyte substitutes may also prove valuable for research. They may be used in studies of liver function where the use of products containing red blood cells might interfere with results; to distinguish the physiologic effects of hormones, enzymes, and blood buffers; as well as to investigate erythropoiesis itself (25)(26)(27).…”

mentioning

confidence: 99%