Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism

Oladipo, Olusola O.; Ayo, Joseph Olusegun; Ambali, Suleiman Folorunsho; Mohammed, Bisalla

doi:10.1080/15376516.2016.1223242

Cited by 31 publications

(14 citation statements)

References 52 publications

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“…Reports by Alya et al indicated that MDA levels and SOD activity in the liver and kidney increased significantly in female rats when exposed to 50 mg Pb/l for 40 and 65 days. More recently, a study reported that hepatic MDA levels were significantly increased in male Wistar rats after exposure to 1.4 mg/kg BW Pb acetate for 15 weeks . Consistent with these results, we observed that chronic exposure to low doses of Pb not only increased the hepatic ROS and MDA levels but also altered gene expression of antioxidant enzymes impacting their activities, implicating oxidative stress involvement in Pb‐induced hepatotoxicity.…”

Section: Discussionsupporting

confidence: 90%

Chronic exposure to low doses of Pb induces hepatotoxicity at the physiological, biochemical, and transcriptomic levels of mice

Luo

Shen

Zhou

et al. 2019

Environmental Toxicology

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Lead (Pb), a non-essential heavy metal, is a major global environmental contaminant with serious toxicological consequences. In the present study, the effects on hepatotoxicity of mice with chronic exposure to low doses of Pb were evaluated. While oral exposure to 0.03 or 0.1 mg/L Pb for 15 weeks in male adult mice had no significant effect on body weights, Pb exposure resulted in liver histopathological effects and increase of hepatic activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). In addition, hepatic reactive oxygen species (ROS) and malondialdehyde (MDA) significantly accumulated after treatment. Conversely, glutathione (GSH) decreased significantly in both 0.03 and 0.1 mg/L Pb-treated groups. Moreover, the hepatic activities of superoxide dismutase 1 (SOD) and catalase (CAT) increased significantly following treatment with 0.1 mg/L Pb for 15 weeks, concomitant with increases in transcriptions of hepatic Sod, Cat, and Gpx. Furthermore, transcriptions of hepatic metallothionein (MT), zinc transporter 5 (Znt5) and copper transporter 1 (Ctr1), and subsequent protein levels were also increased in liver of mice when exposed to 0.1 mg/L Pb for 15 weeks. In addition, the transcriptome data showed that Pb has substantial influence on several pathways, including PPAR signaling pathways, AMPK signaling pathways, fatty acid metabolism, and drug metabolism. Our data suggested that chronic Pb exposure could induce hepatotoxicity at the physiological, biochemical, and transcriptomic levels in mice. K E Y W O R D Shepatotoxicity, oxidative stress, gene expression, Pb

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Section: Discussionsupporting

confidence: 90%

Chronic exposure to low doses of Pb induces hepatotoxicity at the physiological, biochemical, and transcriptomic levels of mice

Luo

Shen

Zhou

et al. 2019

Environmental Toxicology

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“…As one of the most easily accumulated poisonous substances in human body, lead exposure is closely related to chronic renal injury10111920. Early diagnosis of lead-induced kidney damage still lacks appropriate methods with great accuracy and precision, but is critical for long term prognosis in workers with occupational lead exposure142122.…”

Section: Discussionmentioning

confidence: 99%

Urinary KIM-1: a novel biomarker for evaluation of occupational exposure to lead

Zhou

Shen

et al. 2016

Sci Rep

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Chronic occult lead poisoning often develops ensuing occupational lead exposure. Early diagnosis of lead poisoning is critical for timely discontinuation of lead exposure and for prognosis. This study explored the value of urinary kidney injury molecule-1 (KIM-1) in diagnosing renal injury induced by lead at an early stage. We retrospectively analyzed 92 workers exposed to occupational lead and demonstrated a better correlation ship between blood lead levels and urine excretion of KIM-1 than other traditional renal injury biomarkers following creatinine adjustment. Receiver operating characteristic curve analysis of the ability of diverse biomarkers for predicting kidney injury in lead-exposed workers demonstrated that the order of predicting accuracy of the studied biomarkers is as follows: urinary KIM-1-to-creatinine ratio > urinary N-acetyl-β-(D)-glucosaminidase-to-creatinine ratio > urinary β2-microglobulin-to-creatinine ratio > urinary α1-microglobulin-to-creatinine ratio, with the Youden index being 16.59 ng/g, 14.01 U/g, 0.15 mg/g, and 4.63 mg/g, respectively. Collectively, our findings suggest that short-period occupational lead exposure may cause injury of renal tubules. Urinary excretion of KIM-1 correlates with blood lead levels better than other traditional renal injury biomarkers, including N-acetyl-β-(D)-glucosaminidase, α1-microglobulin, and β2-microglobulin. Longitudinal surveillance of urinary KIM-1 may aid for early diagnosis of renal tubular injury in workers with occupational lead exposure.

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“…As demonstrated in recent studies (Oladipo et al ., 2016 ; Dumkova et al ., 2016 ), histological lesions had a diagnostic role only in the renal samples in which Cd concentrations were higher than 0.75 mg/kg. Therefore, at concentrations of Cd ranging from 0.75 to 0.95 mg/kg, the lesions were limited to proximal contorted tubules; while the lesions involved the whole morpho-functional unit of the kidney as the metal concentration increased.…”

Section: Discussionmentioning

confidence: 99%

Presence of cadmium residues in muscle, liver and kidney of Bubalus bubalis and histological evidence

et al. 2018

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Cadmium (Cd) concentrations were evaluated in the samples of kidney, liver and muscle of sixty-six buffaloes regularly slaughtered. Forty were raised in Campania, in the territory between the province of Naples and Caserta and twenty-six were bred in Apulia, in the province of Bari. Two aliquots were prepared for the renal and hepatic samples: one intended for the chemical analysis and the other one intended for histological investigations. Muscle samples were the subject of purely chemical investigation. In the group of forty animals raised in the Campania region, the limits imposed by EC Reg. 1881/2006 and EC Reg. 488/2014 were exceeded in three renal samples, which showed values of 1.53, 1.22 and 1.1 mg/kg respectively; in three hepatic samples, which presented values of 0.72, 0.64 and 0.61 mg/kg, and in five muscle samples, with values of 0.16, 0.16, 0.09, 0.08 and 0.07, respectively. On the other hand, in the group of animals raised and slaughtered in the province of Bari, none of the twenty-six samples examined exceeded the limits imposed by the European regulations. The histological analysis showed typical, but not pathognomonic lesions in the renal samples from the animals raised in the provinces of Naples and Caserta. The levels of Cd contamination found in the samples examined suggest that it would be correct to exclude from the human consumption, as it happens for the equines, the kidney and the liver, especially from the animals raised in some geographical areas with a high rate of pollution.

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Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism

Cited by 31 publications

References 52 publications

Chronic exposure to low doses of Pb induces hepatotoxicity at the physiological, biochemical, and transcriptomic levels of mice

Chronic exposure to low doses of Pb induces hepatotoxicity at the physiological, biochemical, and transcriptomic levels of mice

Urinary KIM-1: a novel biomarker for evaluation of occupational exposure to lead

Presence of cadmium residues in muscle, liver and kidney of Bubalus bubalis and histological evidence

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