Evaluation of hexose and pentose in pre-cultivation of Candida guilliermondii on the key enzymes for xylitol production in sugarcane hemicellulosic hydrolysate

Abstract

The evaluation of hexose and pentose in pre-cultivation of Candida guilliermondii FTI 20037 yeast on xylose reductase (XR) and xylitol dehydrogenase (XDH) enzymes activities was performed during fermentation in sugarcane bagasse hemicellulosic hydrolysate. The xylitol production was evaluated by using cells previously growth in 30.0 gl(-1) xylose, 30.0 gl(-1) glucose and in both sugars mixture (30.0 gl(-1) xylose and 2.0 gl(-1) glucose). The vacuum evaporated hydrolysate (80 gl(-1)) was detoxificated by ion exchange resin (A-860S; A500PS and C-150-Purolite®). The total phenolic compounds and acetic acid were 93.0 and 64.9%, respectively, removed by the resin hydrolysate treatment. All experiments were carried out in Erlenmeyer flasks at 200 rpm, 30°C. The maximum XR (0.618 Umg (Prot) (-1)) and XDH (0.783 Umg (Prot) (-1)) enzymes activities was obtained using inoculum previously growth in both sugars mixture. The highest cell concentration (10.6 gl(-1)) was obtained with inoculum pre-cultivated in the glucose. However, the xylitol yield and xylitol volumetric productivity were favored using the xylose as carbon source. In this case, it was observed maximum xylose (81%) and acetic acid (100%) consumption. It is very important to point out that maximum enzymatic activities were obtained when the mixture of sugars was used as carbon source of inoculum, while the highest fermentative parameters were obtained when xylose was used.

Xylitol production by yeasts isolated from rotting wood in the Galápagos Islands, Ecuador, and description of Cyberlindnera galapagoensis f.a., sp. nov.

“…This behaviour can be explained by differences in the composition of each culture medium. In addition to D-xylose, sugarcane bagasse hydrolysates contain other sugars such as glucose and arabinose, as well as inorganic compounds such as manganese, iron, magnesium, nickel, calcium, sodium, potassium and chromium, and vitamins and amino acids (Arruda et al 2011). All of these compounds could serve as macro and micronutrients for yeast growth by increasing the xylitol production.…”

“…For the production of xylitol via biotechnology, hemicellulose hydrolysates obtained from lignocellulose materials can be used as substrates (Arruda et al 2011;Mohamad et al 2015). Lignocellulose materials are composed of the carbohydrate polymers cellulose (35-50 %) and hemicellulose (20-35 %) and an amorphous tridimensional macromolecule, lignin (10-25 %) (Saha 2003).…”

Xylitol production from cashew apple bagasse by Kluyveromyces marxianus CCA510

“…Xylose, along with glucose, is a major sugar derived from the hydrolysis of lignocellulosic biomass, among other sugars such as mannose, galactose, arabinose and rhamnose (all of them with countless applications in both pharmaceutical and food industries) [10]. d-xylose is the second most abundant sugar in nature, and a process that can be performed to convert xylose to xylitol, a polyol with wide applicability in the food and pharmaceutical industries [11], plays a significant economic role regarding biomass employment [12].…”

Xylose recovery from dilute-acid hydrolysis of oil palm (Elaeis guineensis) empty fruit bunches for xylitol production

“…Arruda et al (2011) performed a fermentation in Erlenmeyer flasks (125 ml) containing 50 ml of the medium (pH 5.5), on a rotary shaker at 200 rpm, 30°C for 120 h and used C. guilliermondii yeast in sugarcane bagasse hemicellulosic hydrolysate with 70 g L -1 xylose. They reported that the xylitol production and Yp/s were 40 g L -1 and 0.6 g g -1 , respectively.…”