Evaluation of highly conserved <i>hsp</i>65-specific nested PCR primers for diagnosing <i>Mycobacterium tuberculosis</i>

Priyadarshini, P.; Tiwari, Karuna; Das, Arghya; Kumar, Deepak; Mishra, Mohini; Desikan, Prabha; Nath, Gopal

doi:10.5588/ijtld.16.0343

Cited by 3 publications

(3 citation statements)

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“…These difficulties mean that alternative, more rapid detection methods for mycobacteria are often used. PCR has proven to be a powerful tool for the specific detection of mycobacterial signature DNA sequences from a variety of sample matrices (Dinnes et al , ; Priyadarshini et al , ), and most recently loop‐mediated isothermal amplification (LAMP) methods have been successfully developed for the specific detection of MAP (Punati et al ., ). However, when used as a diagnostic test, efficient release of DNA is crucial for the success of any PCR or nucleic acid‐based method, especially when this is being used to quantify the number of cells present in a sample.…”

Section: Introductionmentioning

confidence: 99%

The development and use of Actiphage^® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals

Swift

Meade

Barron

et al. 2019

Microbial Biotechnology

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Summary Here, we describe the development of a method that exploits bacteriophage D29 as a lysis agent for efficient DNA extraction from low numbers of mycobacterial cells. This method (Actiphage®) used in combination with PCR achieved rapid and sensitive (LOD ≤ 10 cell ml−1) detection and identification of viable, pathogenic mycobacteria in blood samples within 6 h. We demonstrate that mycobacteriophage D29 can be used to detect a range of mycobacteria from clinical blood samples including both Mycobacterium tuberculosis complex and Mycobacterium avium subsp. paratuberculosis without the need for culture and confirms our earlier observations that a low‐level bacteraemia is associated with these infections in cattle. In a study of M. bovis‐infected cattle (n = 41), the sensitivity of the Actiphage® method was 95 % (95 % CI; 0.84–0.99) and specificity was 100 % (95% CI; 0.92–1). We further used Actiphage® to demonstrate viable Mycobacterium avium subsp. paratuberculosis is present in the blood of Johne’s infected cattle. This method provides a revolutionary new tool for the study of infections caused by these difficult to grow pathogens.

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Section: Introductionmentioning

confidence: 99%

The development and use of Actiphage^® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals

Swift

Meade

Barron

et al. 2019

Microbial Biotechnology

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“…The basic properties of mNPs have been leveraged to create colorimetric sensing arrays with a potential for fast analysis that are cost-effective and easy to implement due to their naked-eye observation capability (Sun et al, 2020). Biosensors based on predictable color changes include but are not limited to biosensors for detecting alpha-1-feto protein in human serum antihepatitis-B virus antibodies in human serum breast cancer biomarkers Mycobacterium tuberculosis complex, human immunodeficiency virus type-1, DNA toxic metal pollutants (Priyadarshini et al, 2017), and organochlorine endosulfan pesticide (ESP) (Arora, 2018). Silver nanoparticles have also been incorporated into polymers to be used in sensors; Kariuki et al (2016) incorporated silver nanoparticles into poly(amic acid) (PAA) polymer matrix nanoparticle-based sensors for nitrobenzene detection.…”

Section: Nanobiosensorsmentioning

confidence: 99%

Amelioration in nanobiosensors for the control of plant diseases: current status and future challenges

Virk,

Deepak,

Taneja

et al. 2024

Front. Nanotechnol.

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The increase in global population has had a tremendous impact on sustainable agri-food practices. With the growth in world population, various modern technologies are being utilized that more often result in the opening of tremendous opportunities in the agriculture and food sectors. Nanotechnology is used in agri-food sectors for a variety of purposes, including enhancing flavor, pest/pathogen diagnosis, production, processing, storage, packaging, and transportation of agricultural products. Plant pathogenic microorganisms including bacteria, viruses, fungi, and nematodes have a significant impact on the global economy. In particular, advances in nanotechnology, including nanobiosensors, have been used in the detection of plant diseases and pathogens, the evaluation and examination of infections caused by microorganisms, the management of diseases and, thus, the promotion of food security. Apart from the management of plant diseases, nanobiosensors offer better opportunities for sustainable agri-food production by controlling physical, chemical, and biological processes, thus improving food safety and the agricultural economy. This review outlines the application of nano-integrated nanobiosensors for better agricultural and food practices.

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“…Generally, to identify pathogen bacteria, partial species-specific nucleotide sequences (molecular markers), such as 16s rRNA ( [11,12]), rnpB ([13]), gyrB ([14]), hsp65 ( [15]), from pathogenic bacteria can be targeted. Conventional pyrosequencing has been widely used to detect pathogenic bacteria by targeting these molecular markers.…”

Section: The Principle For Identification Of Pathogenic Bacteria Using Pdbamentioning

confidence: 99%

Pyrosequencing with di-base addition for the identification of pathogenic bacteria

Shu

2021

E3S Web Conf.

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We proposed a novel method for the identification of pathogenic bacteria, in which a partial amplicon of the molecular markers was targeted by using pyrosequencing with di-base addition (PDBA). PDBA was conducted by synchronously adding di-base instead of one base into a reaction, and a set of highly sequence-specific encodings containing the type and the number of incorporated nucleotide(s) (peak height) were obtained. By comparing the encoding sizes of each isolate and the number of incorporated nucleotide(s) in each cycle, moving from first to last, various kinds of bacteria could be unambiguously identified. To verify its feasibility, we simulated PDBA results from thirteen isolates of Mycobacterium species and compared their encoding sizes and the number of incorporated nucleotide(s) in each cycle with those predicted by a homemade software. The thirteen isolates were successfully differentiated. We also targeted partial RNase P RNA gene (rnpB) of cultured M. paratubercuosis and M. celatum to differentiate the two isolates. By comparing the encoding size of each isolate and the number of incorporated nucleotide(s) in each cycle, the two Mycobacterium isolates were successfully differentiated. In conclusion, PDBA enabled to reliably identify pathogenic bacteria.

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Evaluation of highly conserved hsp65-specific nested PCR primers for diagnosing Mycobacterium tuberculosis

Cited by 3 publications

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The development and use of Actiphage^® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals

The development and use of Actiphage^® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals

Amelioration in nanobiosensors for the control of plant diseases: current status and future challenges

Pyrosequencing with di-base addition for the identification of pathogenic bacteria

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Evaluation of highly conserved hsp65-specific nested PCR primers for diagnosing Mycobacterium tuberculosis

Cited by 3 publications

References 0 publications

The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals

The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals

Amelioration in nanobiosensors for the control of plant diseases: current status and future challenges

Pyrosequencing with di-base addition for the identification of pathogenic bacteria

Contact Info

Product

Resources

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The development and use of Actiphage^® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals

The development and use of Actiphage^® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals