Evaluation of histamine-induced conjunctival oedema in guinea pigs by means of image analysis

Takahashi, Ayako; Sumi, Tamaki; Tada, Kazuya; Mibu, Hisakazu; Shii, Daisuke; Kayasuga, A; Fukushima, Atsuki

doi:10.1136/bjo.2010.180422

Cited by 7 publications

(10 citation statements)

References 16 publications

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“…This study was approved by the Animal Care Committee of Kochi Medical School (K-00118), and the procedures were performed according to the guidelines of the Association for Research in Vision and Ophthalmology Statement for the use of Animals in Ophthalmic and Vision Research . In vivo performance analysis of the EH-SCL was performed based on the previously reported animal model of allergic conjunctivitis [12]. In this animal model, the immediate phase of allergic conjunctivitis is induced by histamine eye drops, and the magnitude of the allergic reaction is measured by the extravasated EB, which was injected intravenously before the histamine challenge.…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo performance of epinastine hydrochloride-releasing contact lenses

et al. 2019

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A number of drug-releasing contact lenses are currently being studied to address issues inherent in eye drops as a drug delivery method. In this study, we developed epinastine hydrochloride-releasing daily soft contact lenses for treatment of allergic conjunctivitis and examined their in vitro and in vivo performance. Preformed soft contact lenses with/without ionic functional groups were soaked in a solution of epinastine hydrochloride in phosphate-buffered saline to prepare epinastine hydrochloride-releasing soft contact lenses. Among these contact lenses with different ionicities, anionic lenses demonstrated the maximum, relatively linear epinastine hydrochloride release, in vitro. The amount of epinastine hydrochloride release was directly proportional to the concentration of the epinastine hydrochloride solution used to prepare the contact lens. The epinastine hydrochloride-releasing anionic soft contact lens also demonstrated prolonged drug release and significantly greater efficacy compared with epinastine hydrochloride eye drops 12 h after treatment, in vivo. Further studies are required to determine the appropriate amount of epinastine hydrochloride to be contained in the anionic soft contact lenses.

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Section: Methodsmentioning

confidence: 99%

In vitro and in vivo performance of epinastine hydrochloride-releasing contact lenses

et al. 2019

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“…Disruption of the blood-tear barrier is also described in human patients with spontaneous ocular surface disorders (e.g., dry eye, allergic conjunctivitis) 226,305-307 and other animal species (rabbits, horses, and guinea pigs). 164,308,309 Increased vascular permeability and disruption of tight junctions between conjunctival epithelial cells likely play a role ( Figure 6), 310,311 although the exact etiopathogenesis is unknown and require further investigation. A few noteworthy limitations are listed here: (i) the model is not adequate to study ocular allergy given the lack of characteristic features noted in canine patients with allergies (e.g., follicular conjunctivitis); (ii) proinflammatory mediators other than histamine are also responsible for triggering conjunctival inflammation in clinical patients (e.g., leukotrienes, cytokines); (iii) conjunctival inflammation is relatively short-lived (115-390 min) and cannot mimic the physiological changes noted in patients with chronic conjunctivitis (e.g., reduced goblet cell density).…”

Section: Histamine-induced Conjunctivitismentioning

confidence: 99%

An eye on the dog as the scientist's best friend for translational research in ophthalmology: Focus on the ocular surface

Sebbag

Mochel

2020

Medicinal Research Reviews

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Preclinical animal studies provide valuable opportunities to better understand human diseases and contribute to major advances in medicine. This review provides a comprehensive overview of ocular parameters in humans and selected animals, with a focus on the ocular surface, detailing species differences in ocular surface anatomy, physiology, tear film dynamics and tear film composition. We describe major pitfalls that tremendously limit the translational potential of traditional laboratory animals (i.e., rabbits, mice, and rats) in ophthalmic research, and highlight the benefits of integrating companion dogs with clinical analogues to human diseases into preclinical pharmacology studies. This One Health approach can help accelerate and improve the framework in which ophthalmic research is translated to the human clinic. Studies can be conducted in canine subjects with naturally occurring or noninvasively induced ocular surface disorders (e.g., dry eye disease, conjunctivitis), reviewed herein, and tear fluid can be easily retrieved from canine eyes for various bioanalytical purposes. In this review, we discuss common tear collection methods, including capillary tubes and Schirmer tear strips, and provide guidelines for tear sampling and extraction to improve the reliability of analyte quantification (drugs, proteins, others).

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“…Earlier, Fukushima and Tomita [21] and Takahashi et al [22] reported that image analyses can be used to quantitatively evaluate the conjunctival hyperaemia and oedema in guinea pigs with histamine-induced hyperaemia or an allergic reaction. However, their methods cannot be easily used in humans because they require the injection of histamine.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Allergic Conjunctivitis by Thermography

Hara

Shiraishi²,

Yamaguchi³

et al. 2014

Ophthalmic Res

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Purpose: To evaluate ocular surface temperature in assessing the efficacy of anti-allergic eye drops. Methods: Thirteen asymptomatic patients (24.7 ± 2.8 years) with proven seasonal allergic conjunctivitis due to cedar pollen were studied. A 0.025% levocabastine ophthalmic suspension was instilled in one eye (levocabastine eye) and artificial tears in the other eye (artificial tear eye) in a masked fashion 10 min prior to a conjunctival allergen challenge (CAC). Then, a drop of cedar pollen solution was dropped into the conjunctival sac to induce the allergic reaction. The surface temperature of the inferior bulbar conjunctiva was measured before and 30 min after the CAC with a newly developed non-contact ocular surface thermographer (OST). The degree of conjunctival injection and chemosis was also determined by slit-lamp biomicroscopy. The changes in the symptoms were evaluated by a questionnaire. Results: After the CAC, the temperature increased by 0.67 ± 0.10°C in the artificial tear eyes but by only 0.21 ± 0.06°C in the levocabastine eyes (p < 0.05). The score for conjunctival injection was 1.38 ± 0.24 and the chemosis score was 0.85 ± 0.25 for the artificial tear eyes and 0.62 ± 0.27 and 0.08 ± 0.08 in the levocabastine eyes (p < 0.01). The temperature increase was significantly correlated with the conjunctival injection scores (r = 0.63; p < 0.001). Conclusion: The significant correlation of the conjunctival surface temperature with the severity of the conjunctival allergic reaction indicates that the temperature measured by the OST can be used to objectively evaluate the efficacy of topical anti-allergic agents.

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Evaluation of histamine-induced conjunctival oedema in guinea pigs by means of image analysis

Abstract: This is the first study to have quantitatively evaluated kinetic changes in histamine-induced bulbar oedema by means of image analysis.

Cited by 7 publications

References 16 publications

In vitro and in vivo performance of epinastine hydrochloride-releasing contact lenses

In vitro and in vivo performance of epinastine hydrochloride-releasing contact lenses

An eye on the dog as the scientist's best friend for translational research in ophthalmology: Focus on the ocular surface

Evaluation of Allergic Conjunctivitis by Thermography

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