Evaluation of HIV serial and parallel serologic testing algorithms in Abidjan, Côte d′Ivoire

Nkengasong, John N.; Maurice, Chantal; Koblavi, S.; Kalou, Mireille; Yavo, Daniel; Maran, M.; Bilé, Ebi; N'guessan, Kabran; Kouadio, J; Bony, Séka; Wiktor, Stefan Z.; Greenberg, Alan E.

doi:10.1097/00002030-199901140-00015

Cited by 52 publications

(24 citation statements)

References 18 publications

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“…Invalid results were reported as indeterminate, defined for the Determine assay as a weak or missing control band. All sera were also tested by a highly sensitive and specific algorithm that uses a combination of two ELISAs (Enzygnost Anti-HIV1/2 Plus [Behring Diagnostic, Marburg, Germany] and ICE 1.0.2 [Abbott, Murex, Dartford, United Kingdom]) for the serodiagnosis of HIV infection (11). In this testing algorithm, sera concordantly reactive or nonreactive by Enzygnost and ICE 1.0.2 were considered true positive or true negative, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…In this testing algorithm, sera concordantly reactive or nonreactive by Enzygnost and ICE 1.0.2 were considered true positive or true negative, respectively. Sera with discordant results in the two assays were tested with the Vironostika HIV Uni-Form II Plus O (Organon Teknika bv, Boxtel, The Netherlands), and the outcome was considered definitive (11). The results of this ELISA-based testing algorithm were used as a "gold standard" for evaluating the rapid assays.…”

Section: Methodsmentioning

confidence: 99%

“…These sera were from different populations: 909 pregnant women, 102 tuberculosis patients, and 205 patients seeking care in an HIV outpatient clinic. These samples had been tested by a highly sensitive and specific ELISAbased algorithm (10,11). Of the 1,216 sera, 793 (65.2%) were HIV negative and 423 (34.8%) were HIV positive.…”

Section: Methodsmentioning

confidence: 99%

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Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast

et al. 2001

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To evaluate serologic testing algorithms for human immunodeficiency virus (HIV) based on a combination of rapid assays among persons with HIV-1 (non-B subtypes) infection, HIV-2 infection, and HIV-1-HIV-2 dual infections in Abidjan, Ivory Coast, a total of 1,216 sera with known HIV serologic status were used to evaluate the sensitivity and specificity of four rapid assays: Determine HIV-1/2, Capillus HIV-1/HIV-2, HIV-SPOT, and Genie II HIV-1/HIV-2. Two serum panels obtained from patients recently infected with HIV-1 subtypes B and non-B were also included. Based on sensitivity and specificity, three of the four rapid assays were evaluated prospectively in parallel (serum samples tested by two simultaneous rapid assays) and serial (serum samples tested by two consecutive rapid assays) testing algorithms. All assays were 100% sensitive, and specificities ranged from 99.4 to 100%. In the prospective evaluation, both the parallel and serial algorithms were 100% sensitive and specific. Our results suggest that rapid assays have high sensitivity and specificity and, when used in parallel or serial testing algorithms, yield results similar to those of enzyme-linked immunosorbent assay-based testing strategies. HIV serodiagnosis based on rapid assays may be a valuable alternative in implementing HIV prevention and surveillance programs in areas where sophisticated laboratories are difficult to establish.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast

et al. 2001

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“…HIV antibody testing is critical for the diagnosis and counseling of HIV-infected persons, the monitoring of trends in HIV prevalence, and the evaluation of the effectiveness of HIV prevention programs (5,12). An unprecedented number of tests for the detection of HIV antibodies are available.…”

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confidence: 99%

Evaluation and Diagnostic Usefulness of Domestic and Imported Enzyme-Linked Immunosorbent Assays for Detection of Human Immunodeficiency Virus Type 1 Antibody in India

Iqbal

Solomon

Murugavel

et al. 2005

Clin Vaccine Immunol

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Diagnosis of human immunodeficiency virus (HIV) infection is importantInfection with human immunodeficiency virus (HIV) has become pandemic since its first documentation in 1981 and is a major public health concern (11). HIV antibody testing is critical for the diagnosis and counseling of HIV-infected persons, the monitoring of trends in HIV prevalence, and the evaluation of the effectiveness of HIV prevention programs (5,12). An unprecedented number of tests for the detection of HIV antibodies are available. In some kits, improved sensitivity is frequently accompanied by a decreased specificity. This has been of particular concern with the introduction of test kits that detect all isotypes of antibodies, such as those based on antibody capture by antigens on a solid phase with labeled antigens as the detecting reagents (4,8).In resource-poor developing countries, the surveillance and diagnosis of HIV infection are major challenges (15). The conventional algorithm for HIV diagnostic testing consists of screening with enzyme immunoassays followed by confirmation with a Western blot test. Moreover, a double enzymelinked immunosorbent assay (ELISA) without Western blotting has been accepted as the customary screening assay for HIV infection (18). Because of the high cost of the Western blot test, it has not been affordable in a number of laboratories in developing countries (1). Rapid screening for HIV infection performed on-site with tests that do not require expensive laboratory infrastructure or highly skilled personnel helps with the diagnoses of patients in emergencies (13). The present study has been designed to evaluate five different commercially available diagnostic ELISA kits, and also a rapid test kit, for their performance in diagnosing HIV infection. MATERIALS AND METHODSThis study was carried out at the Y. R. Gaitonde Centre for AIDS Research and Education (YRG CARE) in Chennai, India; it is a referral center for voluntary counseling and testing (VCT) in South India. A total of 264 specimens (plasma and serum) collected from VCT clients were tested using various commercial HIV ELISA kits, and the positive specimens were confirmed by Western blot analysis (Genetic Systems HIV-1 Western blot; Bio-Rad Laboratories, Redmond, WA). The following commercially available ELISA kits were employed in this study: Enzaids HIV 1ϩ2 (Span Diagnostics Ltd., Surat, India), HIV-CheX (Xcyton Diagnostics Ltd., Bangalore, India), Murex HIV-1.2.0 (Murex Biotech Limited, Dartford, United Kingdom), Genscreen HIV 1/2 version 2 (Bio-Rad Laboratories, France), and Vironostika HIV Uni-Form II Ag/Ab (BioMérieux, The Netherlands). Along with these, a rapid test kit, CombAids RS Advantage (Span Diagnostics Ltd., Surat, India), was also evaluated. A double-blind format was adopted in order to conceal patient information from the testing personnel. One staff member generated duplicate numbers for specimens at the specimen processing section; a second staff member generated plate maps and performed the tests. Finally, the results were ...

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“…HIV infection status was determined using an enzymelinked immunosorbent assay-based parallel testing algorithm (14). CD4 ϩ lymphocytes were enumerated by standard flow cytometry with a FACScan cytometer (Becton Dickinson, San Jose, Calif.), and HIV-1 RNA viral load in plasma was quantified with the Amplicor HIV-1 monitor (version 1.5; Roche Diagnostic Systems, Branchburg, N.J.).…”

Section: Samplesmentioning

confidence: 99%

Field Evaluation of the gag -Based Heteroduplex Mobility Assay for Genetic Subtyping of Circulating Recombinant Forms of Human Immunodeficiency Virus Type 1 in Abidjan, Côte d'Ivoire

et al. 2003

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The gag-based heteroduplex mobility assay (gag-HMA) was evaluated for its ease and reliability in subtyping circulating recombinant forms (CRFs) of human immunodeficiency virus type 1 (HIV-1) in Côte d'Ivoire. One hundred thirty-two plasma samples were analyzed blindly for HIV-1 subtypes by sequencing the pol gene and by gag-HMA. DNA sequencing was used as the "gold standard." Of the 132 samples sequenced, 108 (82%) were CRF02_AG, 14 (11%) were pure subtype A, 5 (4%) were subtype G, 3 (2%) were subtype D, 1 was CRF01_AE, and 1 was subtype H. The gag-HMA correctly classified 126 (95.5%) of the samples. Of the 108 samples that were classified as CRF02_AG by DNA sequencing, 107 (99%) were correctly identified by gag-HMA, resulting in a positive predictive value of 96.4%. The gag-HMA seems to be a valuable tool for understanding the molecular epidemiology of HIV-1 CRF02_AG in Côte d'Ivoire and West Africa, which could be important for developing and evaluating AIDS vaccines, although DNA sequencing remains necessary for accurate molecular epidemiology.

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Evaluation of HIV serial and parallel serologic testing algorithms in Abidjan, Côte d′Ivoire

Abstract: These results suggest that a combination of ELISA using different principles or antigens in a serial or parallel algorithm is an efficient and cost-effective alternative to the standard algorithm in areas where HIV-1 and HIV-2 are prevalent.

Cited by 52 publications

References 18 publications

Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast

Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast

Evaluation and Diagnostic Usefulness of Domestic and Imported Enzyme-Linked Immunosorbent Assays for Detection of Human Immunodeficiency Virus Type 1 Antibody in India

Field Evaluation of the gag -Based Heteroduplex Mobility Assay for Genetic Subtyping of Circulating Recombinant Forms of Human Immunodeficiency Virus Type 1 in Abidjan, Côte d'Ivoire

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