Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts

Caracciolo, Pablo C.; Díaz‐Rodríguez, Patricia; Ardao, Inés; Moreira, David; Montini-Ballarin, Florencia; Abraham, Gustavo Abel; Concheiro, Ángel; Álvarez-Lorenzo, Carmen

doi:10.1016/j.ijbiomac.2021.03.008

Cited by 14 publications

(7 citation statements)

References 73 publications

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“…The prepared grafts exhibited comparable mechanical behavior under dynamic loading cycles to the greater saphenous vein. Zhang et al 193 optimized the thickness and homogeneity of the wall in relation to the compliance of an SDAVG by combining wet spun TPU in the first layer, knitted Spandex middle layer, and again TPU spray coating. Although the developed graft had high strength and elasticity, its compliance did not match the native vessels.…”

Section: Strategies To Prevent Intimal Hyperplasia In Vascular Graftsmentioning

confidence: 99%

Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

2022

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Small-diameter artificial vascular grafts (SDAVG) are used to bypass blood flow in arterial occlusive diseases such as coronary heart or peripheral arterial disease. However, SDAVGs are plagued by restenosis after a short while due to thrombosis and the thickening of the neointimal wall known as intimal hyperplasia (IH). The specific causes of IH have not yet been deduced; however, thrombosis formation due to bioincompatibility as well as a mismatch between the biomechanical properties of the SDAVG and the native artery has been attributed to its initiation. The main challenges that have been faced in fabricating SDAVGs are facilitating rapid re-endothelialization of the luminal surface of the SDAVG and replicating the complex viscoelastic behavior of the arteries. Recent strategies to combat IH formation have been mostly based on imitating the natural structure and function of the native artery (biomimicry). Thus, most recently, developed grafts contain a multilayered structure with a designated function for each layer. This paper reviews the current polymeric, biomimetic SDAVGs in preventing the formation of IH. The materials used in fabrication, challenges, and strategies employed to tackle IH are summarized and discussed, and we focus on the multilayered structure of current SDAVGs. Additionally, the future aspects in this area are pointed out for researchers to consider in their endeavor.

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Section: Strategies To Prevent Intimal Hyperplasia In Vascular Graftsmentioning

confidence: 99%

Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

2022

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Section: Resultsmentioning

confidence: 99%

“…In the 3-day incubation period, the above phenomenon was still observed, indicating that introducing both AP and heparin-loaded hydrogel coating contributed to maintaining the normal physiological viability of HUVECs growing on the AP + EDC/NHS + CS + Hep-PP, owing to the synergistic effect of the anti-inflammatory of AP and the anticoagulant and anti-inflammatory of heparin. 55 The expression of pro-inflammatory cytokine TNF-α and the anti-inflammatory cytokine IL-10 factor in macrophages attached to the surface of the PP samples were assessed by ELISA (Fig. 7E and F).…”

Section: Biomaterials Science Papermentioning

confidence: 99%

Well-designed two-fold crosslinked biological valve leaflets with heparin-loaded hydrogel coating for enhancing anticoagulation, endothelialization, and anticalcification

Yue³

et al. 2022

Biomater. Sci.

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“…There were only a few endothelial cells attached to the G-PP after 24 h or 48 h with the poor morphological phenotype and the growth condition of the cells. The cells of OX-CO-PP, OX-SC-PP and OX-SH-PP were spindle-shaped after 24 h, and the number of cells significantly increased after 48 h with normal morphological phenotype and good growth condition, indicating the good cell compatibility and the enhanced growth of endothelial cells for OX-SH-PP [39,40].…”

Section: Cytotoxicity Of Ppsmentioning

confidence: 96%

Functional non-glutaraldehyde treated porcine pericardium for anti-coagulation, anti-calcification, and endothelial proliferation bioprosthetic heart valves

Chen

Kong

et al. 2022

J Leather Sci Eng

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In the last decade, the number of transcatheter heart valve replacement for severe heart valve disease has increased exponentially. Although the bioprosthetic artificial heart valve (BHV) has similar fluid dynamics performance to the original heart valve compared with mechanical heart valve so that there is no need to take long-term anticoagulant drugs to prevent thromboembolism, transcatheter BHV replacement are still at risk for thrombosis during the first few months according to the clinical data. However, the use of antithrombotic drugs can also increase the risk of bleeding. Therefore, it is particularly important to improve the anticoagulant properties for the BHV itself. In this work, a kind of non-glutaraldehyde cross-linked BHV material with excellent antithrombotic ability has been prepared from carboxylated oxazolidine treated porcine pericardium (consisting of collagen, elastin and glycoprotein) with the further graft of the anticoagulant heparin sodium via hydrophilic modified chitosan. Along with the similar mechanical properties and collagen stability comparable to the glutaraldehyde cross-linked porcine pericardium (PP), these functional non-glutaraldehyde cross-linked PPs exhibit better biocompatibility, promoted endothelial proliferation and superior anti-calcification ability. More importantly, excellent anticoagulant activity can be observed in the hematological experiments in vivo and in vitro. In summary, these excellent performances make these functional non-glutaraldehyde cross-linked PPs great potentialities in the BHV applications. Graphical abstract

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Evaluation of human umbilical vein endothelial cells growth onto heparin-modified electrospun vascular grafts

Cited by 14 publications

References 73 publications

Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

Well-designed two-fold crosslinked biological valve leaflets with heparin-loaded hydrogel coating for enhancing anticoagulation, endothelialization, and anticalcification

Functional non-glutaraldehyde treated porcine pericardium for anti-coagulation, anti-calcification, and endothelial proliferation bioprosthetic heart valves

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