Evaluation of Hypoglycemic and Antioxidative Effects of Synthesized Peptide MC62

Abstract

Diabetes mellitus has been considered as a major health problem in the world today. This study aimed to investigate the hypoglycemic and antioxidative effects of peptide MC62 which was synthesized by solid-phase peptide synthesis method against diabetes induced by streptozotocin. MC62 was administered daily and injected intraperitoneally to the diabetic mice at a dose of 1 μmol/kg body weight for 20 days. The levels of fasting blood glucose and HbA1C, pancreatic islet damage, and associated changes in antioxidative activities were evaluated in streptozotocin-induced diabetic mice used the exenatide as positive control. After the administration of MC62 together with exenatide for 20 days, the elevated fasting blood glucose and HbA1C levels were reduced, and antioxidative activities were restored. It was confirmed with the histological finding that MC62 prevented the islet from damage in diabetic mice. This indicated that MC62 can prevent mice from hyperglycemia which may be associated with oxidative stress. It also suggested that MC62 could be used as a safe alternative hypoglycemic candidate for treatment of diabetes.

Design, synthesis and biological evaluation of novel peptide MC2 analogues from Momordica charantia as potential anti-diabetic agents

“…Consistent with previous reports, 26,31,32 several parameters of global and pancreatic oxidative stress were changed in STZinduced DM mice. Several parameters indicative of oxidative stress in serum (malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-PX), and superoxide dismutase (SOD)) may be altered in STZ-induced DM mice.…”

“…The general procedure of peptide synthesis under microwave irradiation has been reported. [30][31][32] The purity of the crude peptide was 85%. HPLC analyses were undertaken on a 2010-20AT system (Shimadzu, Kyoto, Japan) on a C18 reversed-phase column (5 μm, 250 mm × 4.6 mm) by gradient elution using (A) water with 0.1% TFA-(B) acetonitrile with 0.1% TFA as the mobile phase from 0 min (10% B) to 15 min (90% B).…”

“…The general procedure for cleavage and final deprotection of peptides has been reported. [30][31][32] 3.4 CD spectroscopy studies for I-4 and its analogues III-1-4…”

Novel Pentapeptide GLP‐1 (32–36) Amide Inhibits β‐Cell Apoptosis In Vitro and Improves Glucose Disposal in Streptozotocin‐Induced Diabetic Mice

“…Male KM mice , 6–8 weeks old, weighing 18–22 g, were purchased from Comparative Medicine Centre of Yangzhou University, Jiangsu, China, and housed six per cage under the conditions of ambient temperature (24 ± 1 °C) under a reverse 12‐h light/dark cycle, with free access to food and water. The mice were fasted for overnight before the injection of STZ intraperitoneally (40 mg/kg/day) for 5 consecutive days.…”

Insulin-like plant proteins as potential innovative drugs to treat diabetes—The Moringa oleifera case study

“…Expression of this gene in tandem repetitions improved the yield of the recombinant MC6.1, which had not been satisfactory in a previous study carried out by Liu et al [111]. In addition to this, the hypoglycemic peptide MC6.2, derived from MC6.1 and composed of 11 amino acid residues, was chemically produced by a microwave peptide synthesizer and used in an assay in which it was intraperitoneally administered daily for 20 days to streptozotocin-induced diabetic mice [113]. This daily administration resulted in lower blood glucose levels measured every day after the treatment in comparison with the control group and also improved some symptoms related to hyperglycemia.…”