“…Sample size was calculated using G*Power 3.1.9.2 software with anova : Repeated measures, within factor F ‐test assuming effect size of 15% change in PIP with every 15° change in every position, that is, neutral, flexion 15°, flexion 30°, and flexion 45° (1 group with 4 different measurements). This assumption was based on the results obtained from our previous study, in which PIP mean (95% CI) cm H 2 O in neutral position was 10.4 (9.2–11.3) and 15.2 (13.6–16.7) cm H 2 O with 45° flexion . Correlation was set to be 0.60 for all within factor measures with 80% power of the study and 5% level of significance we had to recruit 50 subjects.…”