Evaluation of Geranium spp., Helleborus spp. and Hyssopus spp. polyphenolic extracts inhibitory activity against urease and α-chymotrypsin

Abstract

This study was meant to determine the inhibitory activity of tannins and flavonoid compounds from Geranium robertianum, Helleborus purpurascens and Hyssopus officinale plant polyphenol rich extracts against urease and a-chymotrypsin. The G. robertianum, H. purpurascens and H. officinale extracts were purified and concentrated by microfiltration and ultrafiltration. Phenolic compounds including flavonoids and tannins have been linked to many pharmacological activities. Thus, the polyphenolic content of the extracts was assessed by UV-Vis spectroscopy and HPLC. The concentrated extracts enriched in polyphenolic compounds (flavonoids, tannins and phenolic acids) showed a significant inhibition against urease from jack bean (over 90%), whereas in case of the a-chymotrypsin, they proved to have an inhibition below 54%. The results of this support the use of G. robertianum, H. purpurascens and H. officinale polyphenolic extracts as potential sources of urease inhibitors. Among the three plant extracts tested, H. officinale polyphenolic extracts exhibited a high inhibitory activity (92.67%) against urease and low inhibition (19.6%) against a-chymotrypsin and could be considered as possible remedy in ulcer treatment.

Chemical characterization and bioactive properties of aqueous and organic extracts of Geranium robertianum L.

“…Ellagic acid has been often detected, sometimes in considerable amounts, in G. robertianum extracts. 7,10,21,31 The phenolic profile of G. robertianum revealed important differences from that of G. molle, previously characterized by our research group. 24 Only four common compounds, i.e., peaks 8, 11, 17 and 18, corresponding to different quercetin and kaempferol glycosides, were observed between these two Geranium species.…”

“…16 Extracts of G. robertianum have shown enzymes' inhibitory activity against urease, α-chymotrypsin and acetylcholinesterase. 5,10 Even though G. robertianum has been used in some folk medicines for the treatment of cancer, 17 the evaluation of its toxicity against cancer cells has been poorly investigated. Only two recent reports on the antitumor potential of aqueous and aqueous/ethanolic extracts of plants native from Romania against human epidermoid laryngeal carcinoma cells (Hep-2p) can be found in the literature.…”

“…7,9 Phytochemical studies of this species have been mainly focused on phenolic compounds from alcoholic or hydroalcoholic extracts, more particularly flavonoids. [7][8][9][10]13,[18][19][20][21] The chemical composition of its essential oils has received much less attention. In the present work, a G. robertianum wild sample was chemically characterized regarding nutritional compositionproteins, fat, carbohydrates and ash -and free sugars, organic acids, fatty acids and tocopherols.…”

<i>In vitro</i> Studies on Anti-diabetic and Anti-ulcer Potentials of <i>Jatropha gossypifolia</i> (Euphorbiaceae)

“…Inhibition (%) = {(Control -Test)/Control} 100 …… (1) where control = total enzyme activity without inhibitor, and test = activity in the presence of test compound.…”

“…Plants have always played a major role in the treatment of human and animal diseases as a therapeutic source for traditional medicine [1]. Enzymes are usually made up of proteins that are helpful in catalyzing chemical reactions.…”