Evaluation of <i>HTLV‐1 HBZ</i> and proviral load, together with host <i>IFN λ3</i>, in pathogenesis of HAM/TSP

Mozhgani, Sayed‐Hamidreza; Jaberi, Najmeh; Rezaee, Seyed Abdolrahim; Bustani, Reza; Jazayeri, Seyed Mohammad; Akbarin, Mohammad Mehdi; Milani, Saeideh; Tarokhian, Hanieh; Norouzi, Mehdi

doi:10.1002/jmv.24721

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…However, no such differences were observed between the HAM/TSP and AC groups. This finding is in contrast with our previous study, in which the IL28B levels were significantly different in the HAM/TSP and AC groups [10]; this might be due to the differences in the clinical patients' status and disease stages. However, these findings revealed a higher expression of IL28B and IL29 in patients infected with a virus such as HTLV-1 than in HCs.…”

Section: Discussioncontrasting

confidence: 99%

“…Double-stranded cDNA was synthesized using the RevertAid TM first strand cDNA synthesis kit (Fermentas, Germany) to determine the expression levels of IL28B [10], IL28R (forward primer: 5ʹ-GAGATCCTGAGTGCCAATGC-3ʹ, reverse primer: 5ʹ-CTGGCTGGAGAGTGATCTGG-3ʹ and TaqMan probe: FAM-CCCCTCCTTCCAGAATGCCACCTCA -BHQ1), IL29 (forward primer: 5ʹ-GGTGACTTTGGTGCTAGGCT-3ʹ, reverse primer: 5ʹ-GGCCTTCTTGAAGCTCGCTA-3ʹ and TaqMan probe: Fam-CCACCACAACTGGGAAGGGCTGCC AC-BHQ1), Tax, and HBZ [14].…”

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

“…They activate hundreds of host genes, whose products have direct antiviral activities [5,7,8]. Type III IFNs, including interleukin (IL)28, IFN-λ 2/3, IL29, and IFN-λ 1 have been shown to be produced after viral infection or by double-stranded RNA [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Interferon Lambda Family along with HTLV-1 Proviral Load, Tax, and HBZ Implicated in the Pathogenesis of Myelopathy/Tropical Spastic Paraparesis

et al. 2018

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HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic neuroinflammatory disease related to human T lymphotropic virus type 1 (HTLV-1) infection. Interferon type III (IFN-λ), which includes IL28, IL29, and IL28R, and affects the outcome of viral infections, might be complicated in the progression of HAM/TSP. Here, we investigated the host-virus interactions in the manifestation of HAM/TSP, using IL28B, IL29, IL28R, HTLV-1 Tax, HTLV-1 basic leucine zipper factor (HBZ), and proviral load (PVL). The study groups consisted of 20 patients with HAM/TSP, 20 asymptomatic HTLV-1 carriers (ACs), and 20 healthy controls (HCs). The means of PVL, Tax, and HBZ gene expressions in the HAM/TSP group (p = 0.004, 0.006, and < 0.0001, respectively) were significantly higher than in the AC group. The comparison of IL28B, IL29, and IL28R expression in the HAM/TSP, AC, and HC groups revealed no significant difference between the first 2, but lower concentrations in the HCs (IL28B: p = 0.03, 0.01; IL29: p = 0.07, 0.01; and IL28R: p < 0.0001, respectively). In the HAM/TSP group, correlations were seen between Tax and HBZ (R = 0.61, p = 0.004) and between Tax and IL29 (R = 0.45, p = 0.04). Negative correlations were observed between Tax and IL28B (R = –0.49, p = 0.02) and between HBZ and IL28R (R = –0.43, p = 0.06). In the ACs, an inverse correlation was found between Tax and IL28B (R = –0.42, p = 0.06). These findings suggest that IL29, IL28B, and IL28R interfere in the infection of HAM/TSP, mainly via Tax activation.

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Section: Discussioncontrasting

confidence: 99%

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

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Interferon Lambda Family along with HTLV-1 Proviral Load, Tax, and HBZ Implicated in the Pathogenesis of Myelopathy/Tropical Spastic Paraparesis

et al. 2018

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“…Interestingly, our data showed that HTLV-1 infection induced the expression of IFN-l and Ku70 knockdown resulted in decreased expression of IFN-l, which has been demonstrated to be important in restricting viral infections (34). It is suggested that IFN-l3 expression was significantly higher in HTLV-1-associated myelopathy/tropical spastic paraparesis patients than in HTLV-1 asymptomatic carriers (35). However, the role of IFN-l in HTLV-1 infections is not well understood and needs further examinations.…”

Section: Discussionmentioning

confidence: 63%

Ku70 Senses HTLV-1 DNA and Modulates HTLV-1 Replication

Wang

Kang

Song

et al. 2017

The Journal of Immunology

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Human T lymphotropic virus type 1 (HTLV-1) belongs to the deltaretrovirus family and has been linked to multiple diseases. However, the innate host defense against HTLV-1 is unclear. In this study, we report that the expression of Ku70, a known DNA sensor against DNA viruses, could be induced by HTLV-1 infection in HeLa, PMA-differentiated THP1 cells, primary human monocytes, and human monocyte-derived macrophages. In these cells, the overexpression of Ku70 inhibited the HTLV-1 protein expression, whereas the knockdown of Ku70 promoted the HTLV-1 protein expression. Furthermore, the overexpression of Ku70 enhanced the cellular response to HTLV-1 infection, whereas Ku70 knockdown yielded the opposite effect. Additionally, Ku70 was found to interact with HTLV-1 reverse transcription intermediate ssDNA90. ssDNA90 stimulation induced Ku70 expression and Ku70 promoted ssDNA90-triggered innate immune responses. Finally, HTLV-1 infection enhanced the association between Ku70 and stimulator of IFN genes, suggesting that stimulator of IFN genes was involved in Ku70-mediated host defenses against HTLV-1 infection. Taken together, our findings suggest a new sensor that detects HTLV-1 reverse transcription intermediate and controls HTLV-1 replication. These findings may provide new angles to understand host defenses against HTLV-1 infection and HTLV-1-associated diseases.

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“…The infection by HIV-1 results in the acquired immunodeficiency syndrome (AIDS) associated with CD 4 þ T cell depletion which results in many mortal diseases [7,8]. HTLV-1 causes two main diseases including Adult T-cell leukaemia/lymphoma (ATLL) and HTLV-1 Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) and also some other disorders [9,10,11]. Moreover, the retroviral coinfection has been reported frequently in recent years [12,13].…”

Section: Introductionmentioning

confidence: 99%

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

Mozhgani

Kermani

Norouzi

et al. 2020

Heliyon

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Early detection of retroviruses including human T-cell lymphotropic virus and human immunodeficiency virus in the human body is indispensable to prevent retroviral infection propagation and improve clinical treatment. Until now, diverse techniques have been employed for the early detection of viruses. Traditional methods are timeconsuming, resource-intensive, and laborious performing. Therefore, designing and constructing a selective and sensitive diagnosis system to detect serious diseases is highly demanded. Genetic detection with high sensitivity has striking significance for the early detection and remedy of disparate pathogenic diseases. The nucleic acid biosensors are based on the identification of specific DNA sequences in biological samples. Nanotechnology has an important impact on the development of sensitive biosensors. Different kinds of nanomaterials include nanoparticles, nanoclusters, quantum dots, carbon nanotubes, nanocomposites, etc., with different properties have been used to improve the performance of biosensors. Recently, DNA nanobiosensors are developed to provide simple, fast, selective, low-cost, and sensitive detection of infectious diseases. In this paper, the research progresses of nano genosensors for the detection of HIV-1 and HTLV-1 viruses, based on electrochemical, optical, and photoelectrochemical platforms are overviewed.

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Evaluation of HTLV‐1 HBZ and proviral load, together with host IFN λ3, in pathogenesis of HAM/TSP

Cited by 8 publications

References 40 publications

Interferon Lambda Family along with HTLV-1 Proviral Load, Tax, and HBZ Implicated in the Pathogenesis of Myelopathy/Tropical Spastic Paraparesis

Interferon Lambda Family along with HTLV-1 Proviral Load, Tax, and HBZ Implicated in the Pathogenesis of Myelopathy/Tropical Spastic Paraparesis

Ku70 Senses HTLV-1 DNA and Modulates HTLV-1 Replication

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

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