Evaluation of <i>In Vitro</i> Antioxidant Properties of Methanol and Aqueous Extracts of <i>Parkinsonia aculeata</i> L. Leaves

Sharma, Sonia; Vig, Adarsh Pal

doi:10.1155/2013/604865

Cited by 50 publications

(41 citation statements)

References 17 publications

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“…While the developed antioxidant sensor was characterized with GA, the obtained antioxidant capacity of the tested herbal extracts can be validated by comparing with the antioxidant activity of GA since this approach was done in many studies [47][48][49][50]. Using the standard calibration curve of GA obtained by scanometry technique, the antioxidant capacity of each extract was calculated and expressed as gallic acid equivalent in mg/L (mg/L GAE) as described in our previous work [29].…”

Section: Application Of Antioxidant Sensor On the Plant Extractsmentioning

confidence: 99%

Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Kuswandi¹,

Sari²,

Hidayat³

2018

jrp

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How to cite this article: Hidayat MA, Sari P, Kuswandi B. Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts. Marmara Pharm J. ABSTRACT: A simple scanometric assay for antioxidant capacity of the herbal extracts has been developed based on dry reagent of 2,2-diphenyl-1-picrylhydrazyl (DPPH) immobilized on the pharmaceutical blister. The deep violet color of DPPH solution was regenerated by introducing methanol to each well as sensing zones. The antioxidant capacity of plant extract will proportionally reduce the violet color to form the corresponding yellow color adduct, where the color change was captured by a flatbed scanner. Then, the optical responses toward antioxidant are presented as an averaged value of the red green blue (RGB) color using image color analysis (ImageJ). By using this color value, analytical performance of the antioxidant sensor could be characterized, where it has a linear range between 1-28 mg/L as gallic acid equivalent (GAE), with response time at 10 min. The reproducibility of the antioxidant sensor was good (RSD < 1%) with the recovery value of 102-105%. The scanometric assay was applied to several herbal extracts, such as sappan wood, guava leaf, and turmeric rhizome. The results obtained are in good agreement with the spectrophotometric method, suggesting that the proposed assay can be used as an alternative method for antioxidant capacity determination.

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Section: Application Of Antioxidant Sensor On the Plant Extractsmentioning

confidence: 99%

Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Kuswandi¹,

Sari²,

Hidayat³

2018

jrp

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“…On the basis of chromatogram obtained for all the samples Vig 2013 and, it was concluded that extract/fractions of P. aculeata leaves contain gallic acid, catechin, chlorogenic acid, epicatechin, caffeic acid, umbelliferone, coumaric acid, rutin, ellagic acid, etc. (Table 9).…”

Section: Antimutagenic Activitymentioning

confidence: 99%

“…aculeata L. is a perennial flowering tree from the family Fabaceae. It is widely cultivated in South America, Mexico, South to North Argentina and now introduced and cultivated in South Africa, Uganda and India also (Wagner et al 1999;Pier 2000;Divya et al 2011;Sharma and Vig 2013). Leaves of this plant are prescribed for malaria, fever and as an abortifacient.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antimutagenic and protective effects of Parkinsonia aculeata L. leaves against H2O2 induced damage in pBR322 DNA

Sharma

Vig

2016

Physiol Mol Biol Plants

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The in vitro antimutagenic and DNA protecting potential of organic (methanol, hexane, n-butanol) and aqueous extract/fractions of Parkinsonia aculeata L. (Fabaceae) was investigated by employing Ames assay and DNA nicking assay. DNA damage by hydroxyl radicals was effectively inhibited by all the extract/fractions. A marked antimutagenic effect was observed against 4-Nitro-o-phenylenediamine and sodium azide (direct acting mutagens) and 2-Aminofluorene (indirect acting mutagen) in TA98 and TA100 strains of Salmonella typhimurium. In Ames assay, two different modes of experiments i.e. pre-incubation and co-incubation were performed and it was observed that all the extract/fractions showed better results in the pre-incubation as compared to co-incubation mode. Out of all the extract/fractions tested, n-butanol fraction was found to be the most effective in preventing DNA damage and inhibiting mutagenesis. UHPLC analysis of extract/fractions revealed presence of polyphenols such as gallic acid, catechin, chlorogenic acid, caffeic acid, umbelliferone, coumaric acid, rutin, and ellagic acid etc. DNA protecting and antimutagenic activity of this plant could be attributed to presence of these polyphenols. The results of this study indicate the presence of potent antioxidant factors in Parkinsonia aculeata L, which are being explored further for their mechanism of action.

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“…Reactive oxygen species (ROS) or free radicals are harmful products or intermediates that may be generated during the processes of biological combustion (Sharma and Vig, 2013;Zaman et al, 2016). The imbalance between oxidants and antioxidants are called oxidative stress, because of the damage in all kinds of biomolecules such as protein, nucleic acid, DNA and RNA (Sharma and Vig, 2013).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant potential and cytotoxicity of Randia dumetorum Lam. leaf extract

Abdullah-Al-Ragib¹,

Hossain²,

Hossain³

et al. 2017

J. Pharmacognosy Phytother.

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The aim of the current study was to explore the antioxidant potential and cytotoxicity of different 'fractions from the leaf extract of Randia dumetorum. Proximate analysis and phytochemical screening were done with standard protocol. The antioxidant activity was evaluated by using reducing power assay, total antioxidant capacity determination, determination of total phenolic content, determination of total flavonoid content and reduction of ferric ions by ortho-phenanthroline color method. In the antioxidant studies, ascorbic acid, gallic acid and butylated hydroxy toluene (BHT) were used as standard antioxidant compound. The brine shrimp lethality test was used to examine cytotoxicity. In the proximate analysis, moisture content, total ash value, acid insoluble ash and water-soluble ash value were found in the leaf of R. dumetorum. Concerning the phytochemical screening, there was the presence of glycosides, flavonoids, reducing sugars, saponins, phenolic compounds, tannins on different fractions, but the absence of gums and mucilage, alkaloid, protein, and amino acid. The results show that all the extracts of R. dumetorum leaf possess significant antioxidant activity. 'The methanol and water extracts of the leaves showed significant cytotoxic activity as compared to dimethyl sulfoxide (DMSO) (LC 50 1.07 µg/ml). These findings suggest that R. dumetorum leaves might be a good antioxidants source and possess mild cytotoxic effect.

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Evaluation of In Vitro Antioxidant Properties of Methanol and Aqueous Extracts of Parkinsonia aculeata L. Leaves

Cited by 50 publications

References 17 publications

Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Evaluation of antimutagenic and protective effects of Parkinsonia aculeata L. leaves against H2O2 induced damage in pBR322 DNA

Antioxidant potential and cytotoxicity of Randia dumetorum Lam. leaf extract

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