Evaluation of Schizochytrium meal in microdiets of Pacific white shrimp ( Litopenaeus vannamei ) larvae

Abstract

A feeding trial was conducted to assess the effects of dietary Schizochytrium meal supplementation on survival, growth performance, activities of digestive enzymes and fatty acid composition in Pacific white shrimp (Litopenaeus vannamei) larvae (initial body weight 4.21 ± 0.10 mg). Four isonitrogenous and isolipidic diets were formulated to contain graded levels of Schizochytrium meal: 0% (S0, the control diet), 2% (S2), 4% (S4) and 6% dry matter (S6). Results showed that there was no significant difference in survival of shrimps among dietary treatments (P > 0.05). Shrimps fed diets with 2% and 4% microalgae meal had significantly higher specific growth rate (SGR) than that of shrimps fed diets with 0% and 6% microalgae meal, and no significant differences were observed between shrimps fed diets with 2% and 4% microalgae meal (P > 0.05). Activity of trypsin in the pancreatic and intestinal segments, and activity of amylase in the pancreatic segments were not significantly affected by dietary microalgae meal levels (P > 0.05). Specific activities of both alkaline phosphatase and leucine‐aminopeptidase in intestine and purified brush border membrane of intestine were significantly higher in shrimps fed diet with 2% microalgae meal (P < 0.05). There were no significant differences in C18:2n‐6, n‐3 fatty acids, n‐6fatty acids, PUFA and n‐3/n‐6 in muscle samples among dietary treatments. C16:1n‐7, C18:1n‐9, MUFA, C18:3n‐3 and C20:5n‐3 decreased, however, C20:4n‐6 increased in the muscle as dietary microalgae meal level increased. In conclusion, 4% Schizochytrium meal in microdiets of shrimps can improve growth performance and may be a valuable additive in the microdiets of shrimps.

Replacement of fish oil withSchizochytriummeal and its impacts on the growth and lipid metabolism of Pacific white shrimp (Litopenaeus vannamei)

“…supplementation (58.7 g/kg) could replace FO up to 75% and enhance growth of shrimp. These results are consistent with studies on the growth performance of Pacific white shrimp larvae fed 20–40 g/kg dietary Schizochytrium meal (Wang et al., ) and channel catfish, Ictalurus punctatus , fed 20 g/kg dried Schizochytrium sp. (Li, Robinson, Tucker, Manning, & Khoo, ).…”

“…Taken together, these results suggest that inclusion of algal meal up to 58.7 g/kg with VO could modify lipid metabolism through modulating the levels of lipase, adipocytes and TC in Pacific white shrimp. Similarly, in other study, dietary Schizochytrium meal (2%) enhanced the specific activities of both alkaline phosphatase and leucine‐aminopeptidase in Pacific white shrimp (Wang et al., ). Dietary Schizochytrium meal also improved the activities of digestive enzyme in Pacific white shrimp (Wang et al., ).…”

“…Microalgae are recognized as prominent and sustainable sources of n‐3 LC‐PUFA‐rich oils that may be a viable alternative to FO and FM as food‐grade fisheries have already reached their limit of sustainability (Heal & Schlenker, ; Hilborn et al., ; Pike & Barlow, ; Wang et al., , ). Dietary Schizochytrium meal increased the level of C20:4n‐6 in the muscle of Pacific white shrimp larvae (Wang et al., , ). Dried Schizochytrium sp.…”

“…At the same time, six shrimp from each tank were randomly selected and anesthetized in an ice bath (0°C), and samples (haemolymph and hepatopancreas) were collected according to Zhou, Wang, Wang, and Tan (2013) and Wang et al (2017aWang et al ( , 2017b and stored at −20°C until analysis.…”

“…Evaluating the nutritional requirements and nutrient metabolism of this species has been the highest priority to produce nutritionally balanced, cost‐effective and environmentally friendly diets. Previous study reported that 40 g/kg Schizochytrium meal in microdiets could improve the growth performance of Pacific white shrimp larvae and enhanced specific enzyme activities, such as alkaline phosphatase and leucine‐aminopeptidase (Wang et al., ). Wang et al.…”