Evaluation of <i><scp>HOXC</scp>8</i> in crested Swiss chicken

Joller, Sara; Ammann, Philippe; Flury, Christine; Drögemüller, Cord

doi:10.1111/age.12674

Cited by 5 publications

(4 citation statements)

References 4 publications

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“…Slight differences appeared in ways of expressing the Crest phenotype (Bartels, 2003). For example, unlike a full crest that grows all over the head as seen in the Silkie chicken and Polish chicken, the Appenzeller Spitzhaubenhuhn chicken expresses a helmet‐like crest, with the feathers pointing up straight from the head (Joller et al, 2018). As mentioned above, the feather crest was reported to be closely related to skull protuberance.…”

Section: Discussionmentioning

confidence: 99%

“…However, Yoshimura et al (2012) proposed a different hypothesis that two different genetic factors separately caused feather crest and cerebral hernia. Moreover, no clear association of three reported Crest markers, HOXC8‐SSR , HOXC8‐3end , and HOXC8‐INTR , was found in Swiss crested chickens, and no polymorphisms were found in exons of HOXC8 (Joller et al, 2018). In addition, Tao et al (2020) suggested that a series of differentially expressed genes (DEGs) existed between crested and noncrested chicken brain regions and eventually identified 547 DEGs, including HOXC10 (Tao et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Chicken HOXC8 and HOXC10 genes may play a role in the altered skull morphology associated with the Crest phenotype

Tsai

Chen

et al. 2023

J Exp Zool Pt B

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One of the most intriguing traits found in domestic chickens is the Crest phenotype. This trait, characterized by a tuft of elongated feathers sprouted from the head, is found in breeds such as Polish chickens and Silkie chickens. Moreover, some crested chicken breeds also exhibit a protuberance in their anterodorsal skull region. Previous studies have strived to identify the causative factors of this trait. This study aimed to elucidate the role of chicken HOXC8 and HOXC10 in the formation of the Crest phenotype. We explored the effect of ectopic expression of HOXC8 or HOXC10 on the chicken craniofacial morphology using the RCAS retrovirus transformation system. Microcomputed tomography scanning was conducted to measure the 3D structure of the cranial bone of transgenic embryos for geometric morphometric analysis. We found that the ectopic expression of HOXC8 or HOXC10 in chicken heads caused mild morphological changes in the skull compared with the GFP‐transgenic control group. Geometric morphometric analysis showed that HOXC8 and HOXC10 transgenic groups expressed a mild upward shape change in the frontal region of the skull compared with the control group, which is similar to what is seen in the crested chicken breeds. In conclusion, this study supports findings in previous studies in which HOX genes play a role in the formation of the altered skull morphology related to the Crest phenotype. It also supports that mutations in HOX genes may contribute to intra‐ and inter‐specific variation in morphological traits in vertebrates.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chicken HOXC8 and HOXC10 genes may play a role in the altered skull morphology associated with the Crest phenotype

Tsai

Chen

et al. 2023

J Exp Zool Pt B

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“…The crest trait is a specific and widely distributed phenotype in birds, but the shape and physiological characteristics of this trait vary among different species of birds. The crest in chickens consists of feathers on the head that are elongated and upraised and differ between breeds in density, size, and shape [7]. The cerebral hemispheres of partially crested chickens are extruded into the spherical region of the skull, and the anterodorsally part of the skull is expanded into a large spherical protuberance, referred to as a "cerebral hernia" [8], but this is not present in all chickens [9].…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Sequencing for Identifying Candidate Genes Related to the Special Phenotypes of the Taihu Dianzi Pigeon

Zhang,

Mu,

Chang

et al. 2024

Animals

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The Taihu Dianzi pigeon is a breed native to China, and its special piebalding, crest, and polydactyly phenotypes are the result of artificial and natural selection. Here, we analyzed the genetic differences among three kinds of pigeons with different phenotypes at the genomic level. A selective sweep was conducted based on the fixation index (FST) and nucleotide diversity (π) ratio, and the results revealed that MC1R was related to the formation of the distinctive piebalding of the Taihu Dianzi pigeon. Combined with the results of genome-wide association studies, we identified candidate genes associated with the crest (SMYD and STOX2) and polydactyly (SLC52A3 and ANGPT4). The candidate genes identified in this study and their variants may be useful for understanding the genetic mechanism underlying the special phenotypes of the Taihu Dianzi pigeon. This study provides new insights into the genetic factors that may influence the formation of the special piebalding, crest, and polydactyly characteristics in pigeons.

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“…In addition, the HOXC8 gene was genotyped with the flanking markers HOXC8 -SSR, HOXC8 -3end, and HOXC11 related to coronal protrusions, and no correlation of the reported markers was found in Swiss crested chicken. The two exons of the HOXC8 gene of crested chicken were sequenced, and no polymorphic sites were found in the coding region ( Joller et al., 2018 ). In summary, the molecular genetic mechanism of the chicken crested trait is still unclear, and further research is urgently needed.…”

Section: Introductionmentioning

confidence: 99%

RNA-sequence reveals differentially expressed genes affecting the crested trait of Wumeng crested chicken

et al. 2021

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Wumeng crested chicken has a cluster of slender feathers on its head, and the underlying skull region exhibits an obvious tumor-like protrusion. This is the typical skull structure of crested chickens. The associated regulatory genes are located on autosomes and are incompletely dominant. This trait is related to brain herniation, but the genetic mechanisms of its formation and development are unclear. In this study, RNA sequencing ( RNA-Seq ) analysis was conducted on 6 skull tissue samples from 3 Wumeng crested chickens with prominent skull protrusions and 3 without a prominent skull protrusion phenotype. A total of 46,376,934 to 43,729,046 clean reads were obtained, the percentage of uniquely mapped reads compared with the reference genome was between 89.73%–91.00%, and 39,795,458–41,836,502 unique reads were obtained. Among different genomic regions, the highest frequency of sequencing reads occurred in exon regions (85.44–88.28%). Additionally, a total of 423 new transcripts and 26,999 alternative splicings ( AS ) events were discovered in this sequencing analysis. This study identified 1,089 differentially expressed genes ( DEGs ), among which 485 were upregulated and 604 were downregulated. Gene Ontology ( GO ) and Kyoto Encyclopedia of Genes and Genomes ( KEGG ) pathway analyses indicated that the DEGs were enriched in terms related to signal transduction, cell development, cell differentiation, the lysosome, serine, and threonine metabolism, and the interaction of cytokines with cytokine receptors. Based on the comprehensive analysis of DEGs combined with reported quantitative trait loci ( QTLs ), the expression of BMP2, EPHA3, EPHB1, HOXC6, SCN2B, BMP7 , and HOXC10 was verified by real-time quantitative polymerase chain reaction ( qRT-PCR ). The qRT-PCR results were consistent with the RNA-Seq results, indicating that these 7 genes may be candidates genes regulating the crested trait.

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Evaluation of HOXC8 in crested Swiss chicken

Cited by 5 publications

References 4 publications

Chicken HOXC8 and HOXC10 genes may play a role in the altered skull morphology associated with the Crest phenotype

Chicken HOXC8 and HOXC10 genes may play a role in the altered skull morphology associated with the Crest phenotype

Whole-Genome Sequencing for Identifying Candidate Genes Related to the Special Phenotypes of the Taihu Dianzi Pigeon

RNA-sequence reveals differentially expressed genes affecting the crested trait of Wumeng crested chicken

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